3D visualization of cellular and molecular distributions in human crystalline lenses at different ages

doi:10.62347/JAMO6905

科研成果详情

题名	3D visualization of cellular and molecular distributions in human crystalline lenses at different ages
作者	Wei Hu 1,2; Xiaoyu Tong 1,2; Lu Zhang3,4; Jiajia Li 1,2; Kaijing Zhou3,4; Wenhan Lu 5; Jiemei Shi 5; Wenjing Wang 3,4; Mengdi Zhang3,4; Yicong Wang 1,2; Wendong Han 6; Liyipeng Chen 7; Jin Li3,4; Yi Feng 1,2
发表日期	2024
发表期刊	American journal of translational research 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	3D visualization Lens cataract optical transparency technology
摘要	The human lens is a highly organized tissue, and it is constructed of delicate inner architectures that ensure its transparency. However, the pattern of cell distribution in the intact lens has rarely been observed or traced in a three-dimensional (3D) perspective., Here, we modified and compared three different kinds of tissue transparency methods to investigate the cellular and molecular changes in the human lens at different ages from a 3D perspective., First, we analyzed the general 3D parameters of cleared human lenses from 6 months to 72 years of age and found that the equator proportion remained constant with age (23.05% ± 0.36). Next, we visualized the cellular distribution patterns in the anterior capsule and equator, as well as the distribution of cortical fiber cells. Interestingly, we observed the accumulation of equatorial epithelium in adolescents and the asymmetrical denucleation of cortical fiber cells in the elderly. Zonula occludens-1 and tropomyosin receptor kinase A were also identified in the pre-equatorial germinative zone, and its presence decreased when comparing lenses of a 17-year-old to those of a 49-year-old., We present a 3D cellular and molecular reconstruction of the human lens, illustrating the observed alterations in human lens epithelial cells across different ages.
ISSN	1943-8141
卷号	16 期号:10
DOI	10.62347/JAMO6905
收录类别	PUBMED
URL	查看原文
PubMed ID	39544736
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/221624
专题	眼视光学院（生物医学工程学院）、附属眼视光医院
作者单位	1.Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences, Institutes of Brain Science, Brain Science Collaborative Innovation Center, State Key Laboratory of Medical Neurobiology, Institute of Acupuncture and Moxibustion, Fudan Institutes of Integrative Medicine, Fudan University Shanghai 200032, China.; 2.Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function Shanghai 200032, China.; 3.National Clinical Research Center for Ocular Diseases, Eye Hospital, Wenzhou Medical University Wenzhou 325027, Zhejiang, China.; 4.State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University Wenzhou 325027, Zhejiang, China.; 5.Department of Ophthalmology and Vision Science, Eye and ENT Hospital, Fudan University Shanghai 200031, China.; 6.Biosafety Level 3 Laboratory and Shanghai Medical College Fudan University Shanghai 200032, China.; 7.Binjiang College of Zhejiang Chinese Medical University Hangzhou 310053, Zhejiang, China.
推荐引用方式 GB/T 7714	Wei Hu,Xiaoyu Tong,Lu Zhang,et al. 3D visualization of cellular and molecular distributions in human crystalline lenses at different ages[J]. American journal of translational research,2024,16(10).
APA	Wei Hu., Xiaoyu Tong., Lu Zhang., Jiajia Li., Kaijing Zhou., ... & Yi Feng. (2024). 3D visualization of cellular and molecular distributions in human crystalline lenses at different ages. American journal of translational research, 16(10).
MLA	Wei Hu,et al."3D visualization of cellular and molecular distributions in human crystalline lenses at different ages".American journal of translational research 16.10(2024).