Nrf2 inhibits M1 macrophage polarization to ameliorate renal ischemia-reperfusion injury through antagonizing NF-κB signaling

doi:10.1016/j.intimp.2024.113310

科研成果详情

题名	Nrf2 inhibits M1 macrophage polarization to ameliorate renal ischemia-reperfusion injury through antagonizing NF-κB signaling
作者	Han, Hui1; Gao, Yuanyuan1; Chen, Boxuan1; Xu, Hongjie1; Shi, Chenghao1; Wang, Xiaowu2; Liang, Yihan3; Wu, Zhixuan1; Wang, Ziqiong1; Bai, Yongheng1; Wu, Cunzao4
发表日期	2024-12-25
发表期刊	International Immunopharmacology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Ischemia-reperfusion injury (IRI) Macrophage polarization Inflammation Nrf2 NF-kappa B
其他关键词	OXIDATIVE STRESS ; KIDNEY INJURY ; PATHOPHYSIOLOGY ; ACTIVATION
摘要	Renal ischemia-reperfusion injury (IRI) is a condition that arises from a sudden interruption of the blood flow to the kidney for a period of time followed by restoration of the blood supply. This process contributes to acute kidney injury (AKI), increases morbidity and mortality, and is a major risk factor for chronic kidney disease (CKD). Nuclear factor erythroid-derived 2-like 2 (Nrf2) has been shown to exhibit strong anti-oxidative and antiinflammatory effects, which are reciprocally regulated by the pro-inflammatory actions of nuclear factor-kappa B (NF-kappa B) kappa B) signaling. In this study, we established a model of AKI caused by renal IRI in mice lacking the Nrf2 gene (KO-Nrf2) and mice pre-injected with ML385 (Nrf2 inhibitor). In addition, LPS- or IL-4-induced M1- or M2-type polarized macrophages (RAW264.7), respectively, were also treated with Nrf2 activation and inhibition. The results demonstrated a more pronounced activation of the NF-kappa B kappa B signaling pathway in the Nrf2 inhibition model, accompanied by a more severe inflammatory effect. In cultured macrophages and renal IRI mice, Nrf2 inhibition activated M1 macrophage polarization, thereby increasing the release of proinflammatory cell factors (iNOS and TNF-alpha) and aggravating renal IRI. Notably, the inhibitory effect of Nrf2 on M1 macrophage polarization was related to the downregulation of the NF-kappa B kappa B signaling pathway activity, resulting in partial relief of renal IRI. Consequently, our findings indicated that Nrf2 inhibits M1 macrophage polarization to ameliorate renal IRI through antagonizing NF-kappa B kappa B signaling. Targeted activation of Nrf2 may be one of the important strategies for renal IRI treatment.
资助项目	Wenzhou Science and Technology Plan Project, China [Y2020207]; Zhejiang Medical and Health Science and Technology project [2024KY147]
出版者	ELSEVIER
ISSN	1567-5769
EISSN	1878-1705
卷号	143 期号:Pt 1
DOI	10.1016/j.intimp.2024.113310
页数	13
WOS类目	Immunology ; Pharmacology & Pharmacy
WOS研究方向	Immunology ; Pharmacology & Pharmacy
WOS记录号	WOS:001332139800001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	39383788
SCOPUSEID	2-s2.0-85205913651
通讯作者地址	[Bai, Yongheng]Wenzhou Med Univ, Affiliated Hosp 1, Zhejiang Key Lab Intelligent Canc Biomarker Discov, Wenzhou 325035, Peoples R China. ; [Wu, Cunzao]Wenzhou Med Univ, Affiliated Hosp 1, Dept Urol, Wenzhou 325035, Peoples R China.
Scopus学科分类	Immunology and Allergy;Immunology;Pharmacology
SCOPUS_ID	SCOPUS_ID:85205913651
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/220784
专题	附属第一医院附属第一医院_泌尿外科附属台州医院其他_温州市第三临床学院（温州市人民医院）其他_附属第三医院（瑞安市人民医院）其他_附属台州医院（浙江省台州医院）
通讯作者	Bai, Yongheng; Wu, Cunzao
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 1, Zhejiang Key Lab Intelligent Canc Biomarker Discov, Wenzhou 325035, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp 3, Dept Burns & Skin Repair Surg, Ruian 325200, Zhejiang, Peoples R China; 3.Wenzhou Med Univ, Taizhou Hosp Zhejiang Prov, Dept Urol, Linhai 317000, Peoples R China; 4.Wenzhou Med Univ, Affiliated Hosp 1, Dept Urol, Wenzhou 325035, Peoples R China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院; 泌尿外科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Han, Hui,Gao, Yuanyuan,Chen, Boxuan,et al. Nrf2 inhibits M1 macrophage polarization to ameliorate renal ischemia-reperfusion injury through antagonizing NF-κB signaling[J]. International Immunopharmacology,2024,143(Pt 1).
APA	Han, Hui., Gao, Yuanyuan., Chen, Boxuan., Xu, Hongjie., Shi, Chenghao., ... & Wu, Cunzao. (2024). Nrf2 inhibits M1 macrophage polarization to ameliorate renal ischemia-reperfusion injury through antagonizing NF-κB signaling. International Immunopharmacology, 143(Pt 1).
MLA	Han, Hui,et al."Nrf2 inhibits M1 macrophage polarization to ameliorate renal ischemia-reperfusion injury through antagonizing NF-κB signaling".International Immunopharmacology 143.Pt 1(2024).