Corynoxine exerts the anti-tumor effect on esophageal squamous cell carcinoma principally via the EZH2-DUSP5-ERK1/2-mediated cell growth inhibition

doi:10.1016/j.phymed.2024.156103

科研成果详情

题名	Corynoxine exerts the anti-tumor effect on esophageal squamous cell carcinoma principally via the EZH2-DUSP5-ERK1/2-mediated cell growth inhibition
作者	Huang, Gang1,2,3; Xu, Jiale1; Li, Yingchao1,4; Song, Liangtao2; Wen, Chunmei1; Ruan, Qingqing 1; Wen, Zhikai1,5; Qi, Jinxia6; Deng, Jie 1; Liu, Yu2
发表日期	2024-12
发表期刊	Phytomedicine 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Corynoxine Squamous cell carcinoma DUSP5 EZH2 p-ERK1/2
其他关键词	CANCER ; EZH2 ; RAF
摘要	Background: Esophageal cancer is one of the most prevalent malignant tumors and the sixth largest cause of tumor-associated death worldwide. Squamous cell carcinoma (ESCC) accounts for 85 % of all esophageal cancer cases. ESCC treatment remains to be significantly difficult. Corynoxine (Cory) is a tetracyclic hydroxyindole alkaloid isolated from Uncaria macrophylla. It is unclear whether Cory has an anti-tumor effect on ESCC. Purpose: To determine the anti-tumor activity of Cory and the associated mechanisms in ESCC. Study design: Cory's effects on proliferation, apoptosis, migration, and invasion, as well as the underlying molecular causes were assessed using two ESCC cell lines, KYSE150 and TE-1. A xenograft mouse model was then applied to evaluate the anti-tumor activity of Cory in vivo. Methods: Western blot, assays including CCK-8, colony formation, EdU staining, TUNEL staining, cell scratch and Transwell, and a xenograft mouse model were used in this study. Results: Cory suppressed cell growth, provoked cell apoptosis, and hindered cell migration and invasion of ESCC cells. DUSP5 knockdown reduced the Cory-induced cell death and restored cell migration and invasion through ERK1/2 activation. Further analyses showed that Cory promoted DUSP5 expression via inhibiting EZH2 expression, leading to inactivation of ERK1/2 signaling and the subsequent cell growth inhibition of ESCC. In vivo experiments disclosed that Cory suppressed tumor growth of ESCC through upregulating DUSP5 expression. Conclusions: Cory plays an anti-tumor role in ESCC by regulating EZH2-DUSP5-ERK1/2 signaling pathway. Cory may be promising to be a novel therapy for treating ESCC.
资助项目	Zhejiang Provincial Natural Science Foundation of China [LY21H160059]; National Natural Science Foundation of China [82102983]
出版者	ELSEVIER GMBH
ISSN	0944-7113
EISSN	1618-095X
卷号	135
DOI	10.1016/j.phymed.2024.156103
页数	10
WOS类目	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS记录号	WOS:001333243600001
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	39383633
SCOPUSEID	2-s2.0-85205676603
通讯作者地址	[Deng, Jie]Wenzhou Med Univ, Zhejiang Key Lab Intelligent Canc Biomarker Discov, Affiliated Hosp 1, Wenzhou 325035, Peoples R China. ; [Liu, Yu]Wenzhou Med Univ, Dept Thorac Surg, Affiliated Hosp 1, Wenzhou 325035, Peoples R China.
Scopus学科分类	Molecular Medicine;Pharmacology;Pharmaceutical Science;Drug Discovery;Complementary and Alternative Medicine
SCOPUS_ID	SCOPUS_ID:85205676603
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/220768
专题	附属第一医院附属第一医院_消化内科附属第一医院_心胸外科
通讯作者	Deng, Jie; Liu, Yu
作者单位	1.Wenzhou Med Univ, Zhejiang Key Lab Intelligent Canc Biomarker Discov, Affiliated Hosp 1, Wenzhou 325035, Peoples R China; 2.Wenzhou Med Univ, Dept Thorac Surg, Affiliated Hosp 1, Wenzhou 325035, Peoples R China; 3.Peoples Hosp Beilun Dist, Ningbo 315000, Peoples R China; 4.Wenzhou Med Univ, Dept Gastroenterol, Affiliated Hosp 1, Wenzhou 325035, Peoples R China; 5.Wenzhou Med Univ, Dept Hepatopancreatobiliary Surg, Affiliated Hosp 1, Wenzhou 325035, Peoples R China; 6.Wenzhou Med Univ, Biobank, Affiliated Hosp 1, Wenzhou 325035, Zhejiang, Peoples R China
第一作者单位	附属第一医院; 心胸外科
通讯作者单位	附属第一医院; 心胸外科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Huang, Gang,Xu, Jiale,Li, Yingchao,et al. Corynoxine exerts the anti-tumor effect on esophageal squamous cell carcinoma principally via the EZH2-DUSP5-ERK1/2-mediated cell growth inhibition[J]. Phytomedicine,2024,135.
APA	Huang, Gang., Xu, Jiale., Li, Yingchao., Song, Liangtao., Wen, Chunmei., ... & Liu, Yu. (2024). Corynoxine exerts the anti-tumor effect on esophageal squamous cell carcinoma principally via the EZH2-DUSP5-ERK1/2-mediated cell growth inhibition. Phytomedicine, 135.
MLA	Huang, Gang,et al."Corynoxine exerts the anti-tumor effect on esophageal squamous cell carcinoma principally via the EZH2-DUSP5-ERK1/2-mediated cell growth inhibition".Phytomedicine 135(2024).