Ferulic acid suppresses the inflammation and apoptosis in Kawasaki disease through activating the AMPK/mTOR/NF-κB pathway

doi:10.3389/fphar.2024.1420602

科研成果详情

题名	Ferulic acid suppresses the inflammation and apoptosis in Kawasaki disease through activating the AMPK/mTOR/NF-κB pathway
作者	Wu, Huilan1,2; Wang, Yijia 1,2; Tan, Pingping1,2; Ran, Yuqing1,2; Guan, Yuting1,2; Qian, Songwei3; Feng, Xing1,2; Jiang, Yalan1,2; Peng, Yongmiao1,2; Sheng, Ke1,2; Xi, Haitao4; Ji, Weiping2,3,5; Guo, Xiaoling1,2,6,7
发表日期	2024-08-29
发表期刊	FRONTIERS IN PHARMACOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	kawasaki disease (KD) ferulic acid (FA) coronary artery vasculitis inflammation apoptosis
其他关键词	PROTEIN-KINASE ; CELLS ; MICE ; MTOR ; EXPRESSION ; MANAGEMENT ; REGULATOR ; ARTERITIS ; GROWTH ; BCL-2
摘要	Background Kawasaki disease (KD) is a self-limiting and acute systemic vasculitis of unknown etiology, mainly affecting children. Ferulic acid (FA), a natural phenolic substance, has multiple pharmacological properties, including anti-inflammatory, anti-apoptosis, and anti-fibrosis, and so on. So far, the protective effects of FA on KD have not been explored.Methods In this study, we established Candida albicans water soluble fraction (CAWS)-induced mouse coronary artery vasculitis of KD model and the tumor necrosis factor alpha (TNF-alpha)-induced human umbilical vein endothelial cells (HUVECs) injury model to investigate the anti-inflammatory and anti-apoptosis effects of FA on KD, and try to elucidate the underlying mechanism.Results Our in vivo results demonstrated that FA exerted anti-inflammatory effects on KD by inhibiting the infiltration of CD45-positive leukocytes and fibrosis around the coronary artery. Additionally, FA downregulated the levels of inflammatory and chemotactic cytokines, alleviated splenomegaly, and exhibited anti-apoptotic effects on KD by reducing TUNEL-positive cells, downregulating BAX expression, and upregulating BCL-2 expression. In addition, Our in vitro findings showed that FA could effectively inhibit TNF-alpha-induced HUVEC inflammation like NF-kappa B inhibitor QNZ by downregulating the expression of pro-inflammatory cytokines as well as attenuated TNF-alpha-induced HUVEC apoptosis by reducing apoptotic cell numbers and the BAX/BCL-2 ratio, which could be reversed by the AMPK inhibitor compound c (CC). The further mechanistic study demonstrated that FA could restrain vascular endothelial cell inflammation and apoptosis in KD through activating the AMPK/mTOR/NF-kappa B pathway. However, FA alone is hard to completely restore KD into normal condition.Conclusion In conclusion, FA has potential protective effects on KD, suggesting its promising role as an adjuvant for KD therapy in the future.
资助项目	Key Research and Development Program of Zhejiang Province [2023C03018]; Natural Science Foundation of Zhejiang Province [LY24H020008, LTGY23H030003]; Fourth Batch of Wenzhou Medical University Outstanding and Excellent Youth Training Project [604090352/640]
出版者	FRONTIERS MEDIA SA
EISSN	1663-9812
卷号	15
DOI	10.3389/fphar.2024.1420602
页数	18
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:001309854900001
收录类别	SCIE
PubMed ID	39268468
通讯作者地址	[Guo, Xiaoling]Wenzhou Med Univ, Affiliated Hosp 2, Basic Med Res Ctr, Wenzhou, Zhejiang, Peoples R China. ; [Ji, Weiping;Guo, Xiaoling]Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou, Zhejiang, Peoples R China. ; [Ji, Weiping]Wenzhou Med Univ, Quzhou Peoples Hosp, Dept Gen Surg, Quzhou Affiliated Hosp, Quzhou, Zhejiang, Peoples R China. ; [Xi, Haitao]Wenzhou Med Univ, Reprod Med Ctr, Dept Obstet & Gynecol, Affiliated Hosp 2, Wenzhou, Zhejiang, Peoples R China. ; [Ji, Weiping]Wenzhou Med Univ, Affiliated Hosp 2, Dept Gen Surg, Wenzhou, Zhejiang, Peoples R China. ; [Guo, Xiaoling]Wenzhou Med Univ, Affiliated Hosp 2, Sci Res Dept, Yuying Childrens Hosp, Wenzhou, Zhejiang, Peoples R China. ; [Guo, Xiaoling]Wenzhou Med Univ, Affiliated Hosp 2, Key Lab Struct Malformat Children Zhejiang Prov, Yuying Childrens Hosp, Wenzhou, Zhejiang, Peoples R China.
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/218761
专题	附属第二医院第二临床医学院、附属第二医院、育英儿童医院其他_温州医科大学附属衢州医院
通讯作者	Xi, Haitao; Ji, Weiping; Guo, Xiaoling
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 2, Basic Med Res Ctr, Wenzhou, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou, Zhejiang, Peoples R China; 3.Wenzhou Med Univ, Quzhou Peoples Hosp, Dept Gen Surg, Quzhou Affiliated Hosp, Quzhou, Zhejiang, Peoples R China; 4.Wenzhou Med Univ, Reprod Med Ctr, Dept Obstet & Gynecol, Affiliated Hosp 2, Wenzhou, Zhejiang, Peoples R China; 5.Wenzhou Med Univ, Affiliated Hosp 2, Dept Gen Surg, Wenzhou, Zhejiang, Peoples R China; 6.Wenzhou Med Univ, Affiliated Hosp 2, Sci Res Dept, Yuying Childrens Hosp, Wenzhou, Zhejiang, Peoples R China; 7.Wenzhou Med Univ, Affiliated Hosp 2, Key Lab Struct Malformat Children Zhejiang Prov, Yuying Childrens Hosp, Wenzhou, Zhejiang, Peoples R China
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院
通讯作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院; 温州医科大学附属衢州医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Wu, Huilan,Wang, Yijia,Tan, Pingping,et al. Ferulic acid suppresses the inflammation and apoptosis in Kawasaki disease through activating the AMPK/mTOR/NF-κB pathway[J]. FRONTIERS IN PHARMACOLOGY,2024,15.
APA	Wu, Huilan., Wang, Yijia., Tan, Pingping., Ran, Yuqing., Guan, Yuting., ... & Guo, Xiaoling. (2024). Ferulic acid suppresses the inflammation and apoptosis in Kawasaki disease through activating the AMPK/mTOR/NF-κB pathway. FRONTIERS IN PHARMACOLOGY, 15.
MLA	Wu, Huilan,et al."Ferulic acid suppresses the inflammation and apoptosis in Kawasaki disease through activating the AMPK/mTOR/NF-κB pathway".FRONTIERS IN PHARMACOLOGY 15(2024).