Katanin regulatory subunit B1 (KATNB1) regulates BTB dynamics through changes in cytoskeletal organization

doi:10.1096/fj.202400966R

科研成果详情

题名	Katanin regulatory subunit B1 (KATNB1) regulates BTB dynamics through changes in cytoskeletal organization
作者	Mao, Bai-Ping1,2,3,4; Pan, Mingdong1,2,3,4; Shan, Yuanyuan 2,5; Wang, Ya-Nan2,6; Li, Huitao1,2; Wu, Jinhan 1,2; Zhu, Xuanjing 1,2; Hu, Ende 1,2,3,4; Cheng, C. Yan 7; Shangguan, Wangning1,2,3,4
发表日期	2024-09-30
发表期刊	FASEB Journal 影响因子和分区
语种	英语
原始文献类型	Article
关键词	blood-testis barrier ectoplasmic specialization F-actin microtubules Sertoli cell spermatogenesis testis
其他关键词	BLOOD-TESTIS BARRIER ; ECTOPLASMIC SPECIALIZATION ; CAMSAP2 ; PROTEIN ; MICROTUBULES ; ATPASE ; ACTIN
摘要	In this study, we have explored the role of the KATNB1 gene, a microtubule-severing protein, in the seminiferous epithelium of the rat testis. Our data have shown that KATNB1 expressed in rat brain, testes, and Sertoli cells. KATNB1 was found to co-localize with alpha-tubulin showing a unique stage-specific distribution across the seminiferous epithelium. Knockdown of KATNB1 by RNAi led to significant disruption of the tight junction (TJ) permeability barrier function in primary Sertoli cells cultured in vitro with an established functional TJ-barrier, as well as perturbations in the microtubule and actin cytoskeleton organization. The disruption in these cytoskeletal structures, in turn, led to improper distribution of TJ and basal ES proteins essential for maintaining the Sertoli TJ function. More importantly, overexpression of KATNB1 in the testis in vivo was found to block cadmium-induced blood-testis barrier (BTB) disruption and testis injury. KATNB1 exerted its promoting effects on BTB and spermatogenesis through corrective spatiotemporal expression of actin- and microtubule-based regulatory proteins by maintaining the proper organization of cytoskeletons in the testis, illustrating its plausible therapeutic implication. In summary, Katanin regulatory subunit B1 (KATNB1) plays a crucial role in BTB and spermatogenesis through its effects on the actin- and microtubule-based cytoskeletons in Sertoli cells and testis, providing important insights into male reproductive biology.
资助项目	Wenzhou Science and Technology Bureau; Natural Science Foundation of Zhejiang [LQ22H040007, LQ22H040008]; [Y20210020]; [Y20210253]
出版者	WILEY
ISSN	0892-6638
EISSN	1530-6860
卷号	38 期号:18
DOI	10.1096/fj.202400966R
页数	17
WOS类目	Biochemistry & Molecular Biology ; Biology ; Cell Biology
WOS研究方向	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology
WOS记录号	WOS:001313287000001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	39275889
SCOPUSEID	2-s2.0-85203986555
通讯作者地址	[Mao, Bai-Ping;Shangguan, Wangning]Wenzhou Med Univ, Affiliated Hosp 2, Dept Anesthesiol & Perioperat Med, Wenzhou 325027, Zhejiang, Peoples R China. ; [Mao, Bai-Ping;Shangguan, Wangning]Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Zhejiang, Peoples R China. ; [Cheng, C. Yan]Zhejiang Univ, Sir Run Run Shaw Hosp, Sch Med, Dept Urol & Androl, Hangzhou 310016, Zhejiang, Peoples R China.
Scopus学科分类	Biotechnology;Biochemistry;Molecular Biology;Genetics
SCOPUS_ID	SCOPUS_ID:85203986555
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/218687
专题	附属第二医院第二临床医学院、附属第二医院、育英儿童医院附属第二医院_病理科第二临床医学院、附属第二医院、育英儿童医院_病理学附属第二医院_麻醉科附属第二医院_麻醉重点学科实验室
通讯作者	Mao, Bai-Ping; Cheng, C. Yan; Shangguan, Wangning
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 2, Dept Anesthesiol & Perioperat Med, Wenzhou 325027, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Zhejiang, Peoples R China; 3.Wenzhou Med Univ, Minist Educ, Key Lab Pediat Anesthesiol, Wenzhou, Peoples R China; 4.Wenzhou Med Univ, Key Lab Anesthesiol Zhejiang Prov, Wenzhou, Peoples R China; 5.Wenzhou Med Univ, Affiliated Hosp 2, Dept Pathol, Wenzhou, Peoples R China; 6.Wenzhou Med Univ, Affiliated Hosp 2, Rehabil Med Ctr, Wenzhou, Peoples R China; 7.Zhejiang Univ, Sir Run Run Shaw Hosp, Sch Med, Dept Urol & Androl, Hangzhou 310016, Zhejiang, Peoples R China
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院; 麻醉科; 温州医科大学; 麻醉重点学科实验室
通讯作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院; 麻醉科
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Mao, Bai-Ping,Pan, Mingdong,Shan, Yuanyuan,et al. Katanin regulatory subunit B1 (KATNB1) regulates BTB dynamics through changes in cytoskeletal organization[J]. FASEB Journal,2024,38(18).
APA	Mao, Bai-Ping., Pan, Mingdong., Shan, Yuanyuan., Wang, Ya-Nan., Li, Huitao., ... & Shangguan, Wangning. (2024). Katanin regulatory subunit B1 (KATNB1) regulates BTB dynamics through changes in cytoskeletal organization. FASEB Journal, 38(18).
MLA	Mao, Bai-Ping,et al."Katanin regulatory subunit B1 (KATNB1) regulates BTB dynamics through changes in cytoskeletal organization".FASEB Journal 38.18(2024).