Electrospun polycaprolactone-chitosan nanofibers on a zinc mesh as biodegradable guided bone-regeneration membranes with enhanced mechanical, antibacterial, and osteogenic properties for alveolar bone-repair applications

doi:10.1016/j.actbio.2024.08.033

科研成果详情

题名	Electrospun polycaprolactone-chitosan nanofibers on a zinc mesh as biodegradable guided bone-regeneration membranes with enhanced mechanical, antibacterial, and osteogenic properties for alveolar bone-repair applications
作者	Wenjie Xu 1; Xue Gao 2; Menghan Zhang 1; Zhengting Jiang1; Xiaomin Xu1; Liangfu Huang1; Huiyu Yao1; Yitian Zhang1; Xian Tong3; Yuncang Li 4; Jixing Lin5; Cuie Wen 6; Xi Ding7
发表日期	2024-08-26
发表期刊	Acta Biomaterialia 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Cytocompatibility Electrospinning GBRM Osteogenic differentiation Zn alloys
其他关键词	IN-VITRO ; COLLAGEN ; AUGMENTATION ; SURFACE ; ALLOY
摘要	Guided bone-regeneration membrane (GBRM) is commonly used in bone-repair surgery because it blocks fibroblast proliferation and provides spatial support in bone-defect spaces. However, the need for removal surgery and the lack of antibacterial properties of conventional GBRM limit its therapeutic applicability for alveolar bone defects. Here we developed a GBRM for alveolar bone-repair and -regeneration applications through double-sided electrospinning of polycaprolactone and chitosan layers on a Zn mesh surface (denoted DSZM). The DSZM showed a UTS of ∼25.6 MPa, elongation of ∼16.1%, strength-elongation product of ∼0.413 GPa%, and ultrahigh spatial maintenance ability, and the UTS was over 6 times higher than that of commercial Bio-Gide membrane. The DSZM exhibited a corrosion rate of ∼17 µm/y and a Zn ion concentration of ∼0.23 μg/ml after 1 month of immersion in Hanks' solution. The DSZM showed direct and indirect cytocompatibility with exceptional osteogenic differentiation and calcium deposition toward MC3T3-E1 cells. Further, the DSZM showed strongly sustained antibacterial activity against S. aureus and osteogenesis in a rat critical-sized maxillary defect model. Overall, the DSZM fits the requirements for alveolar bone-repair and -regeneration applications as a biodegradable GBRM material due to its spatial support, suitable degradability, cytocompatibility, and antibacterial and osteogenic capabilities. STATEMENT OF SIGNIFICANCE: This work reports the mechanical properties, antibacterial ability and osteogenic properties of electrospun PCL-CS nanofiber on Zn mesh as biodegradable guided bone-regeneration membrane for alveolar bone-repair applications. Our findings demonstrate that the DSZM prepared by double-sided electrospinning of PCL-CS layers on Zn mesh showed a UTS of ∼25.6 MPa, elongation of ∼16.1%, strength-elongation product of ∼0.413 GPa%, and ultrahigh spatial maintenance ability, and the UTS was over 6 times greater than that of commercial Bio-Gide® membrane. The DSZM showed direct and indirect cytocompatibility with exceptional osteogenic differentiation and calcium deposition toward MC3T3-E1 cells. Further, the DSZM showed strongly sustained antibacterial activity against S. aureus and osteogenesis in a rat critical-sized maxillary defect model.
资助项目	Wenzhou Major Scientific and Technological Innovation Project [ZY2022022]; Australian Research Council (ARC) through the Discovery Project [DP240101131]; Zhejiang Public Welfare Technology Application Research Project of China [LTGY24H140002]
出版者	Acta Materialia Inc
ISSN	1742-7061
EISSN	1878-7568
卷号	187 页码:434-450
DOI	10.1016/j.actbio.2024.08.033
页数	17
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS研究方向	Engineering ; Materials Science
WOS记录号	WOS:001329635900001
收录类别	PUBMED ; EI ; SCIE ; SCOPUS
EI入藏号	20243717033390
EI主题词	Corrosion rate
EI分类号	101.1 ; 101.2.3 ; 102.1.2.1 ; 103 ; 1301.1.1 ; 1301.4 ; 201.1.2 ; 201.7.1 ; 201.9.1 ; 202.6.3 ; 202.7.2 ; 202.9.1 ; 202.9.3 ; 205.1.1 ; 214.1.2 ; 761 Nanotechnology ; 804.1 Organic Compounds
URL	查看原文
PubMed ID	39197567
SCOPUSEID	2-s2.0-85203540377
通讯作者地址	[Ding, Xi]Wenzhou Med Univ, Affiliated Hosp 1, Wenzhou 325027, Peoples R China. ; [Tong, Xian;Lin, Jixing]Wenzhou Med Univ, Sch & Hosp Stomatol, Inst Stomatol, Wenzhou 325027, Peoples R China. ; [Wen, Cuie]RMIT Univ, Sch Engn, Melbourne, Vic 3001, Australia.
Scopus学科分类	Biotechnology;Biomaterials;Biochemistry;Biomedical Engineering;Molecular Biology
SCOPUS_ID	SCOPUS_ID:85203540377
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/217680
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院附属第二医院第二临床医学院、附属第二医院、育英儿童医院口腔医学院、附属口腔医院口腔医学院、附属口腔医院_口腔医学研究所
作者单位	1.The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China.; 2.The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China.; 3.Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou 325027, China. Electronic address: tx847595271@163.com.; 4.School of Engineering, RMIT University, Melbourne, Victoria 3001, Australia.; 5.Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou 325027, China. Electronic address: jixing.lin@wmu.edu.cn.; 6.School of Engineering, RMIT University, Melbourne, Victoria 3001, Australia. Electronic address: cuie.wen@rmit.edu.au.; 7.The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China. Electronic address: dingxi@wzhospital.cn.
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 口腔医学院、附属口腔医院; 口腔医学研究所
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Wenjie Xu,Xue Gao,Menghan Zhang,et al. Electrospun polycaprolactone-chitosan nanofibers on a zinc mesh as biodegradable guided bone-regeneration membranes with enhanced mechanical, antibacterial, and osteogenic properties for alveolar bone-repair applications[J]. Acta Biomaterialia,2024,187:434-450.
APA	Wenjie Xu., Xue Gao., Menghan Zhang., Zhengting Jiang., Xiaomin Xu., ... & Xi Ding. (2024). Electrospun polycaprolactone-chitosan nanofibers on a zinc mesh as biodegradable guided bone-regeneration membranes with enhanced mechanical, antibacterial, and osteogenic properties for alveolar bone-repair applications. Acta Biomaterialia, 187, 434-450.
MLA	Wenjie Xu,et al."Electrospun polycaprolactone-chitosan nanofibers on a zinc mesh as biodegradable guided bone-regeneration membranes with enhanced mechanical, antibacterial, and osteogenic properties for alveolar bone-repair applications".Acta Biomaterialia 187(2024):434-450.