Vaginal and endometrial microbiome dysbiosis associated with adverse embryo transfer outcomes

doi:10.1186/s12958-024-01274-y

科研成果详情

题名	Vaginal and endometrial microbiome dysbiosis associated with adverse embryo transfer outcomes
作者	Su, Weijue 1; Gong, Chaochao 1; Zhong, Haoyue1; Yang, Huaqing1; Chen, Yuyan1; Wu, Xiaoyuan1; Jin, Jing 2; Xi, Haitao1; Zhao, Junzhao1
发表日期	2024-12-01
发表期刊	Reproductive Biology and Endocrinology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Diagnostic model Implantation failure In vitro fertilization-embryo transfer Infertility Lactobacillus Microbiome Reproductive tract
摘要	Background: Assisted reproductive technology (ART) is the most effective method to treat infertility and the pathogenesis of implantation failure after in vitro fertilization-embryo transfer (IVF-ET) is a challenging filed in infertility. Microbes in the female reproductive tract are considered to be associated with gynecological and obstetric diseases. However, its effects on embryo implantation failure are unsured. Purpose: This study aimed to investigate reproductive tract dysbiosis, identify different bacteria in reproductive tract as potential biomarkers of embryo implantation failure and demonstrate the pathogenesis through metabolites analysis. Methods: We compared the data from 16S rRNA gene and metagenome in reproductive tracts through QIIME2 and HUMAnN2 by the times of embryo implantation failure on 239 infertile patients and 17 healthy women. Results: Our study revealed a strong positive correlation between Lactobacillus abundance and embryo implantation success (IS) after IVF-ET. The microbial community composition and structure in reproductive tract showed substantially difference between the embryo implantation failure (IF) and healthy control. Moreover, we established a diagnostic model through receiver operating characteristic (ROC) with 0.913 area under curve (AUC) in IS and multiple implantation failures (MIF), verified its effectiveness with an AUC = 0.784 demonstrating microbial community alterations could efficiently discriminate MIF patients. Metagenome functional analyses of vaginal samples from another independent infertile patients after IVF-ET revealed the L-lysine synthesis pathway enriched in IF patients, along with ascended vaginal pH and decreased Lactobacillus abundance. Conclusions: This study clarifies several independent relationships of bacteria in vagina and endometrial fluid on embryo implantation failure and undoubtedly broadens the understanding about female reproductive health.
资助项目	Wenzhou Medical University advantageous and distinctive Discipline Construction Project
出版者	BMC
ISSN	1477-7827
EISSN	1477-7827
卷号	22 期号:1
DOI	10.1186/s12958-024-01274-y
页数	12
WOS类目	Endocrinology & Metabolism ; Reproductive Biology
WOS研究方向	Endocrinology & Metabolism ; Reproductive Biology
WOS记录号	WOS:001300716900002
收录类别	SCOPUS ; PUBMED ; SCIE
URL	查看原文
PubMed ID	39198832
SCOPUSEID	2-s2.0-85202145532
通讯作者地址	[Xi, Haitao]Reproductive Medicine Center,Department of Obstetrics and Gynecology,The Second Affiliated Hospital,Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325000,China ; [Zhao, Junzhao]Reproductive Medicine Center,Department of Obstetrics and Gynecology,The Second Affiliated Hospital,Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Reproductive Medicine;Endocrinology;Obstetrics and Gynecology;Developmental Biology
SCOPUS_ID	SCOPUS_ID:85202145532
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/217549
专题	附属第二医院附属第二医院_妇产科
通讯作者	Xi, Haitao; Zhao, Junzhao
作者单位	1.Reproductive Medicine Center,Department of Obstetrics and Gynecology,The Second Affiliated Hospital,Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Laboratory Medicine Center,Zhejiang Center for Clinical Laboratories,Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital),Hangzhou Medical College,Zhejiang,Hangzhou,310000,China
第一作者单位	附属第二医院; 妇产科
通讯作者单位	附属第二医院; 妇产科
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Su, Weijue,Gong, Chaochao,Zhong, Haoyue,et al. Vaginal and endometrial microbiome dysbiosis associated with adverse embryo transfer outcomes[J]. Reproductive Biology and Endocrinology,2024,22(1).
APA	Su, Weijue., Gong, Chaochao., Zhong, Haoyue., Yang, Huaqing., Chen, Yuyan., ... & Zhao, Junzhao. (2024). Vaginal and endometrial microbiome dysbiosis associated with adverse embryo transfer outcomes. Reproductive Biology and Endocrinology, 22(1).
MLA	Su, Weijue,et al."Vaginal and endometrial microbiome dysbiosis associated with adverse embryo transfer outcomes".Reproductive Biology and Endocrinology 22.1(2024).