题名 | Vaginal and endometrial microbiome dysbiosis associated with adverse embryo transfer outcomes |
作者 | |
发表日期 | 2024-12-01 |
发表期刊 | Reproductive Biology and Endocrinology 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
关键词 | Diagnostic model Implantation failure In vitro fertilization-embryo transfer Infertility Lactobacillus Microbiome Reproductive tract |
摘要 | Background: Assisted reproductive technology (ART) is the most effective method to treat infertility and the pathogenesis of implantation failure after in vitro fertilization-embryo transfer (IVF-ET) is a challenging filed in infertility. Microbes in the female reproductive tract are considered to be associated with gynecological and obstetric diseases. However, its effects on embryo implantation failure are unsured. Purpose: This study aimed to investigate reproductive tract dysbiosis, identify different bacteria in reproductive tract as potential biomarkers of embryo implantation failure and demonstrate the pathogenesis through metabolites analysis. Methods: We compared the data from 16S rRNA gene and metagenome in reproductive tracts through QIIME2 and HUMAnN2 by the times of embryo implantation failure on 239 infertile patients and 17 healthy women. Results: Our study revealed a strong positive correlation between Lactobacillus abundance and embryo implantation success (IS) after IVF-ET. The microbial community composition and structure in reproductive tract showed substantially difference between the embryo implantation failure (IF) and healthy control. Moreover, we established a diagnostic model through receiver operating characteristic (ROC) with 0.913 area under curve (AUC) in IS and multiple implantation failures (MIF), verified its effectiveness with an AUC = 0.784 demonstrating microbial community alterations could efficiently discriminate MIF patients. Metagenome functional analyses of vaginal samples from another independent infertile patients after IVF-ET revealed the L-lysine synthesis pathway enriched in IF patients, along with ascended vaginal pH and decreased Lactobacillus abundance. Conclusions: This study clarifies several independent relationships of bacteria in vagina and endometrial fluid on embryo implantation failure and undoubtedly broadens the understanding about female reproductive health. |
资助项目 | Wenzhou Medical University advantageous and distinctive Discipline Construction Project |
出版者 | BMC |
ISSN | 1477-7827 |
EISSN | 1477-7827 |
卷号 | 22期号:1 |
DOI | 10.1186/s12958-024-01274-y |
页数 | 12 |
WOS类目 | Endocrinology & Metabolism ; Reproductive Biology |
WOS研究方向 | Endocrinology & Metabolism ; Reproductive Biology |
WOS记录号 | WOS:001300716900002 |
收录类别 | SCOPUS ; PUBMED ; SCIE |
URL | 查看原文 |
PubMed ID | 39198832 |
SCOPUSEID | 2-s2.0-85202145532 |
通讯作者地址 | [Xi, Haitao]Reproductive Medicine Center,Department of Obstetrics and Gynecology,The Second Affiliated Hospital,Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325000,China ; [Zhao, Junzhao]Reproductive Medicine Center,Department of Obstetrics and Gynecology,The Second Affiliated Hospital,Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325000,China |
Scopus学科分类 | Reproductive Medicine;Endocrinology;Obstetrics and Gynecology;Developmental Biology |
SCOPUS_ID | SCOPUS_ID:85202145532 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/217549 |
专题 | 附属第二医院 附属第二医院_妇产科 |
通讯作者 | Xi, Haitao; Zhao, Junzhao |
作者单位 | 1.Reproductive Medicine Center,Department of Obstetrics and Gynecology,The Second Affiliated Hospital,Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Laboratory Medicine Center,Zhejiang Center for Clinical Laboratories,Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital),Hangzhou Medical College,Zhejiang,Hangzhou,310000,China |
第一作者单位 | 附属第二医院; 妇产科 |
通讯作者单位 | 附属第二医院; 妇产科 |
第一作者的第一单位 | 附属第二医院 |
推荐引用方式 GB/T 7714 | Su, Weijue,Gong, Chaochao,Zhong, Haoyue,et al. Vaginal and endometrial microbiome dysbiosis associated with adverse embryo transfer outcomes[J]. Reproductive Biology and Endocrinology,2024,22(1). |
APA | Su, Weijue., Gong, Chaochao., Zhong, Haoyue., Yang, Huaqing., Chen, Yuyan., ... & Zhao, Junzhao. (2024). Vaginal and endometrial microbiome dysbiosis associated with adverse embryo transfer outcomes. Reproductive Biology and Endocrinology, 22(1). |
MLA | Su, Weijue,et al."Vaginal and endometrial microbiome dysbiosis associated with adverse embryo transfer outcomes".Reproductive Biology and Endocrinology 22.1(2024). |
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