Identification of novel Tet(X6)-Tet(X2) recombinant variant in Elizabethkingia meningoseptica from a bullfrog farm and downstream river in China

doi:10.3389/fmicb.2024.1453801

科研成果详情

题名	Identification of novel Tet(X6)-Tet(X2) recombinant variant in Elizabethkingia meningoseptica from a bullfrog farm and downstream river in China
作者	Jin, Haobo1; Jia, Qing1; Jin, Xi 2; Zhu, Xinlong1; Wang, Min-Ge 3; Sun, Ruan-Yang 4; Cui, Chaoyue1
发表日期	2024-07-31
发表期刊	FRONTIERS IN MICROBIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	tet(X) recombinant variant Elizabethkingia meningoseptica ICE tigecycline
其他关键词	FEATURES
摘要	Introduction: The dissemination of strains producing tetracyclines monooxygenase Tet(X) from breeding farms to the natural environment poses a potential threat to public health. Methods: Antimicrobial susceptibility testing and WGS were performed to identify resistance phenotypes and genotypes. Cloning experiments, sequence alignment, and homology modeling were used to characterize the function and formation mechanisms of the recombinant variant. The mobilization potential of Tet(X) was assessed by collinearity analysis, conjugation experiments, and phylogenetic analysis. Results: Three tet(X)-producing Elizabethkingia meningoseptica strains were isolated from bullfrog breeding ponds, the sewage outlet, and downstream river in Zhejiang Province, China. These strains carry a novel Tet(X) variant, differing from Tet(X6) by seven residues, and possess the ability to degrade tetracyclines. Interestingly, the novel Tet(X) is a recombinant variant formed by homologous recombination of Tet(X6) and the C-terminal of Tet(X2). Further analysis revealed that Tet(X6) formed several Tet(X) variants, including Tet(X5), through homologous recombination. The novel tet(X) gene is located on a circularizable integrative and conjugative element (ICEEmeChn3), with ISwz1 participating in the recombination of its multi-drug resistance region, potentially facilitating the mobilization and recombination of tet(X) in early hosts. These three strains were clonally transmitted and shared a close genetic relationship (SNP < 62) with a clinically-sourced strain isolated from the same province. Discussion: To our knowledge, this is the first report of homologous recombination between Tet(X) variants with differing activities. These clonal strains provide evidence of the transmission of tet(X)-positive strains from aquaculture sewage to the natural environment, highlighting the need to strengthen the monitoring and management of this emerging farming model.
资助项目	Young Scientists Fund of the National Natural Science Foundation of China [32302927]; Basic Public Welfare Research Project of Wenzhou Science and Technology Bureau [2023Y1770]
出版者	FRONTIERS MEDIA SA
EISSN	1664-302X
卷号	15
DOI	10.3389/fmicb.2024.1453801
页数	10
WOS类目	Microbiology
WOS研究方向	Microbiology
WOS记录号	WOS:001291080700001
收录类别	SCIE
PubMed ID	39144213
通讯作者地址	[Cui, Chaoyue]Wenzhou Med Univ, Lab Anim Ctr, Wenzhou, Zhejiang, Peoples R China.
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/217401
专题	仁济学院_实验动物中心附属第二医院
通讯作者	Cui, Chaoyue
作者单位	1.Wenzhou Med Univ, Lab Anim Ctr, Wenzhou, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp 2, Dept Clin Lab, Wenzhou, Zhejiang, Peoples R China; 3.Liaocheng Univ, Phage Res Ctr, Liaocheng, Shandong, Peoples R China; 4.South China Agr Univ, Natl Risk Assessment Lab Antimicrobial Resistance, Guangzhou, Guangdong, Peoples R China
第一作者单位	仁济学院_实验动物中心
通讯作者单位	仁济学院_实验动物中心
第一作者的第一单位	仁济学院_实验动物中心
推荐引用方式 GB/T 7714	Jin, Haobo,Jia, Qing,Jin, Xi,et al. Identification of novel Tet(X6)-Tet(X2) recombinant variant in Elizabethkingia meningoseptica from a bullfrog farm and downstream river in China[J]. FRONTIERS IN MICROBIOLOGY,2024,15.
APA	Jin, Haobo., Jia, Qing., Jin, Xi., Zhu, Xinlong., Wang, Min-Ge., ... & Cui, Chaoyue. (2024). Identification of novel Tet(X6)-Tet(X2) recombinant variant in Elizabethkingia meningoseptica from a bullfrog farm and downstream river in China. FRONTIERS IN MICROBIOLOGY, 15.
MLA	Jin, Haobo,et al."Identification of novel Tet(X6)-Tet(X2) recombinant variant in Elizabethkingia meningoseptica from a bullfrog farm and downstream river in China".FRONTIERS IN MICROBIOLOGY 15(2024).