Vagus nerve stimulation (VNS) inhibits cardiac mast cells activation and improves myocardial atrophy after ischemic stroke

doi:10.1016/j.intimp.2024.112714

科研成果详情

题名	Vagus nerve stimulation (VNS) inhibits cardiac mast cells activation and improves myocardial atrophy after ischemic stroke
作者	Tan, Qianqian1,2,4; Ruan, Yu1; Wu, Shaoqi1,2,4; Jiang, Yong1,2,4; Fu, Rongrong1,2,4; Gu, Xiaoxue1,2,4; Yu, Jiaying1,2,4; Wu, Qiaoyun1,2,4; Li, Ming3; Jiang, Songhe1,2,4
发表日期	2024-09-30
发表期刊	International Immunopharmacology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Ang II Chymase Inflammatory response Ischemic stroke Myocardial atrophy VNS
其他关键词	CEREBRAL-ISCHEMIA
摘要	Background: Ischemic stroke is one of the leading causes of chronic disability worldwide, and stroke-induced heart damage can lead to death. According to research, patients with a variety of brain disease have good clinical results after vagus nerve stimulation (VNS). After ischemic stroke, mast cells (MCs) degranulate and release a large number of mediators, which may cause systemic inflammation. Chymase secreted by MCs can increase the levels of pathological angiotensin II (AngⅡ), which plays a crucial role in the deterioration of heart disease. Our goal was to develop a minimally invasive, targeted, and convenient VNS approach to assess the impact of VNS and to clarify the relationship between VNS and MCs in the prognosis of patients with myocardial atrophy after acute ischemic stroke. Methods: In this study, we verified the role of VNS in the treatment of myocardial atrophy after stroke and its molecular mechanism using a rat model of middle cerebral artery occlusion (MCAO/r). Behavioral studies were assessed using neurobehavioral deficit scores. Enzyme-linked immunosorbent assays, immunofluorescence staining, Western blotting and qRT-PCR were used to analyze the expression levels of myocardial atrophy, MC and inflammatory markers in rat hearts. Results: VNS improved myocardial atrophy in MCAO/r rats, inhibited MC activation, reduced the expression of chymase and AngⅡ, and inhibited the expression of proinflammatory factors. The chymase activator C48/80 reversed these effects of VNS. Chymase activation inhibited the effect of VNS on myocardial atrophy in MCAO/r rats, increased AngⅡ expression and aggravated inflammation and autophagy. The myocardial atrophy of MCAO/r rats was improved after chymase inhibition, and AngⅡ expression, inflammation and autophagy were reduced. Our results suggest that VNS may reduce the expression of chymase and AngⅡ by inhibiting MC activation, thereby improving myocardial atrophy and reducing inflammation and autophagy in MCAO/r rats. Inhibition of MC activation may be an effective strategy for treating myocardial atrophy after stroke. Conclusions: VNS inhibits MC activation and reduces the expression of chymase and AngII, thereby alleviating myocardial atrophy, inflammation and autophagy after stroke.
出版者	ELSEVIER
ISSN	1567-5769
EISSN	1878-1705
卷号	139
DOI	10.1016/j.intimp.2024.112714
页数	12
WOS类目	Immunology ; Pharmacology & Pharmacy
WOS研究方向	Immunology ; Pharmacology & Pharmacy
WOS记录号	WOS:001284278300001
收录类别	SCOPUS ; PUBMED ; SCIE
URL	查看原文
PubMed ID	39068751
SCOPUSEID	2-s2.0-85199677262
通讯作者地址	[Li, Ming]School of Basic Medical Science,Wenzhou Medical University,Zhejiang,Wenzhou,325027,China ; [Jiang, Songhe]Rehabilitation Medicine Center,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China
Scopus学科分类	Immunology and Allergy;Immunology;Pharmacology
SCOPUS_ID	SCOPUS_ID:85199677262
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/216942
专题	第二临床医学院、附属第二医院、育英儿童医院基础医学院（机能实验教学中心）附属第二医院第二临床医学院、附属第二医院、育英儿童医院_康复医学
通讯作者	Li, Ming; Jiang, Songhe
作者单位	1.Rehabilitation Medicine Center,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China; 2.Intelligent Rehabilitation Research Center,International Institute for Acupuncture and Rehabilitation,Wenzhou Medical University,Zhejiang,Wenzhou,325000,China; 3.School of Basic Medical Science,Wenzhou Medical University,Zhejiang,Wenzhou,325027,China; 4.The Provincial Key Laboratory for Acupuncture and Rehabilitation in Zhejiang Province,Key Laboratory for Rehabilitation Research,The Wenzhou,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 康复医学; 温州医科大学
通讯作者单位	基础医学院（机能实验教学中心）; 第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 康复医学
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Tan, Qianqian,Ruan, Yu,Wu, Shaoqi,et al. Vagus nerve stimulation (VNS) inhibits cardiac mast cells activation and improves myocardial atrophy after ischemic stroke[J]. International Immunopharmacology,2024,139.
APA	Tan, Qianqian., Ruan, Yu., Wu, Shaoqi., Jiang, Yong., Fu, Rongrong., ... & Jiang, Songhe. (2024). Vagus nerve stimulation (VNS) inhibits cardiac mast cells activation and improves myocardial atrophy after ischemic stroke. International Immunopharmacology, 139.
MLA	Tan, Qianqian,et al."Vagus nerve stimulation (VNS) inhibits cardiac mast cells activation and improves myocardial atrophy after ischemic stroke".International Immunopharmacology 139(2024).