Cell adhesion molecule 2 inhibits colorectal cancer progression through attenuating epithelial-mesenchymal transition

doi:10.2306/scienceasia1513-1874.2024.070

科研成果详情

题名	Cell adhesion molecule 2 inhibits colorectal cancer progression through attenuating epithelial-mesenchymal transition
作者	Liu, Yangyang 1; Fang, Daoquan1; Fang, Xiaojuan2; Zhong, Zuyue1; Zhang, Qiongying 5; Lian, Yichu6; Shao, Fanggui 3,4; Jiang, Lei1,6
发表日期	2024-06
发表期刊	SCIENCEASIA 影响因子和分区
语种	英语
原始文献类型	Article
关键词	colorectal cancer progression CADM2 epithelial-mesenchymal transition
其他关键词	TUMOR-INFILTRATING LYMPHOCYTES ; SUPPRESSOR GENE ; IMMUNE CELLS ; METHYLATION ; EXPRESSION ; SURVIVAL ; METASTASIS ; CADM2
摘要	: Analysis of colorectal cancer (CRC) data revealed that cell adhesion molecule 2 (CADM2) is lowly expressed in tumor tissues. This study, therefore, aimed to elucidate the anti-oncogenesis roles of CADM2. The expression level CADM2 in The Cancer Genome Atlas (TCGA) were analyzed. We validated the expression level in adjacent normal, tumor, and metastatic tissues through real-time polymerase chain reaction (PCR) and immunohistochemistry (IHC). Methylation-specific PCR was used to explore the reason for the low expression. The biological function of CADM2 was verified by analyzing CCK-8 and transwell in cell line. Bioinformatics and Western blot analyses were used to explore anti-tumor progression pathways and the differences in immune infiltration levels. We found that CADM2 is weakly expressed in the TCGA and has good diagnostic performance. The low expression of CADM2 in cell lines and tissues was confirmed, and the hypoexpression was correlated with promoter hypermethylation. The IHC showed statistically significant decreased expression in primary focal compared to adjacent normal tissue, which further reduced in metastatic foci compared with primary foci. Up-regulation of CADM2 inhibited the growth and migrative and invasive ability of cells. When CADM2 expression was up-regulated, significant changes occurred in the expression of epithelialmesenchymal transition (EMT)-related protein. Bioinformatics analysis indicated that the molecular mechanism of CADM2 inhibiting tumor progression may also be related to the EMT process. Moreover, the CADM2 high-expression subgroup had higher immunity scores and infiltration levels. The research showed the association of CADM2 with carcinogenesis and metastasis by EMT process, providing new insight for molecular therapy and diagnosis of CRC.
资助项目	Wen-zhou Municipal Science and Technology Bureau of China [Y20210742]; Zhejiang Provincial Natural Science Foun-dation of China [LQ22H200003, LY24H160024]; Medical and Health Science and Technology Program of Zhe-jiang Province [2024KY1243]; Fundamental Scientific Re-search Fees of Wenzhou Medical University [KYYW2021026]; National Natural Science Foundation of China [82272652]
出版者	SCIENCE SOCIETY THAILAND
ISSN	1513-1874
卷号	50 期号:3
DOI	10.2306/scienceasia1513-1874.2024.070
页数	11
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:001279043000001
收录类别	SCIE
通讯作者地址	[Jiang, Lei]Wenzhou Med Univ, Affiliated Hosp 1, Cent Lab, Wenzhou 325000, Zhejiang, Peoples R China. ; [Shao, Fanggui]Wenzhou Med Univ, Dept Lab Med, Affiliated Hosp 1, Wenzhou ZHEJIANG, Zhejiang, Peoples R China. ; [Shao, Fanggui]Wenzhou Med Univ, Dept Clin Lab, Key Lab Clin Lab Diag & Translat Res Zhejiang Prov, Affiliated Hosp 1, Wenzhou 325000, Zhejiang, Peoples R China. ; [Jiang, Lei]Wenzhou Med Univ, Cixi Biomed Res Inst, Wenzhou 315000, Zhejiang, Peoples R China.
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/216869
专题	第一临床医学院（信息与工程学院）、附属第一医院_中心实验室附属第一医院附属第一医院_病理科附属第一医院_检验科其他_温州医科大学慈溪生物医药研究院
通讯作者	Shao, Fanggui; Jiang, Lei
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 1, Cent Lab, Wenzhou 325000, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp 1, Key Lab Intelligent Treatment & Life Support Crit, Wenzhou 325000, Zhejiang, Peoples R China; 3.Wenzhou Med Univ, Dept Lab Med, Affiliated Hosp 1, Wenzhou ZHEJIANG, Zhejiang, Peoples R China; 4.Wenzhou Med Univ, Dept Clin Lab, Key Lab Clin Lab Diag & Translat Res Zhejiang Prov, Affiliated Hosp 1, Wenzhou 325000, Zhejiang, Peoples R China; 5.Wenzhou Med Univ, Affiliated Hosp 1, Dept Pathol, Wenzhou 325000, Zhejiang, Peoples R China; 6.Wenzhou Med Univ, Cixi Biomed Res Inst, Wenzhou 315000, Zhejiang, Peoples R China
第一作者单位	第一临床医学院（信息与工程学院）、附属第一医院_中心实验室
通讯作者单位	第一临床医学院（信息与工程学院）、附属第一医院_中心实验室; 附属第一医院_检验科; 附属第一医院; 其他_温州医科大学慈溪生物医药研究院
第一作者的第一单位	第一临床医学院（信息与工程学院）、附属第一医院_中心实验室
推荐引用方式 GB/T 7714	Liu, Yangyang,Fang, Daoquan,Fang, Xiaojuan,et al. Cell adhesion molecule 2 inhibits colorectal cancer progression through attenuating epithelial-mesenchymal transition[J]. SCIENCEASIA,2024,50(3).
APA	Liu, Yangyang., Fang, Daoquan., Fang, Xiaojuan., Zhong, Zuyue., Zhang, Qiongying., ... & Jiang, Lei. (2024). Cell adhesion molecule 2 inhibits colorectal cancer progression through attenuating epithelial-mesenchymal transition. SCIENCEASIA, 50(3).
MLA	Liu, Yangyang,et al."Cell adhesion molecule 2 inhibits colorectal cancer progression through attenuating epithelial-mesenchymal transition".SCIENCEASIA 50.3(2024).