H2O2 promotes photodynamic efficacy of TMPyP4 against ovarian cancer in vitro by downregulating HIF-1α expression

doi:10.1016/j.biopha.2024.117110

科研成果详情

题名	H2O2 promotes photodynamic efficacy of TMPyP4 against ovarian cancer in vitro by downregulating HIF-1α expression
作者	Chen, Kejie1,2; Zhai, Yihui2; Wang, Yuanqiu1; Xu, Zichuang2; Chen, Xiaojian2; Zhang, Yixin 1,3; Zhou, Zhengyi 1,3; Zheng, Xiaohui2; Lin, Feng 1
发表日期	2024-08
发表期刊	BIOMEDICINE & PHARMACOTHERAPY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	PDT Hypoxia Ovarian cancer TMPyP4 HIF-1 alpha
其他关键词	DNA-DAMAGE ; REPAIR ; CELLS ; NANOPARTICLES ; GENERATION ; RESISTANCE ; APOPTOSIS ; THERAPY ; RNA
摘要	Photodynamic therapy (PDT), employing photosensitizers to induce formation of reactive oxygen species (ROS) for tumor elimination, is emerging as a promising treatment modality in oncology due to its unique benefits. However, the PDT application in ovarian cancer, the most prevalent and lethal type of gynecological malignancy with a severe hypoxic microenvironment, remains unknown. This study revealed that photosensitizer TMPyP4 exhibited enhanced efficacy under H2O2 stimulation, with minimal change in cytotoxicity compared to TMPyP4 alone. The results showed that H2O2 increased ROS production induced by TMPyP4, leading to exacerbated mitochondrial dysfunction and DNA damage, ultimately inhibiting proliferation and inducing apoptosis in ovarian cancer cells. Mechanistically, H2O2 primarily enhanced the therapeutic efficacy of PDT with TMPyP4 against ovarian cancer cells by degrading HIF-1 alpha, which subsequently modulated the HIF-1 signaling pathway, thereby alleviating the hypoxic environment in ovarian cancer cells. Our findings underscore the therapeutic potential of targeting HIF-1 alpha within the hypoxic microenvironment for PDT in ovarian cancer and propose a novel integrated strategy for PDT treatment of this malignancy in vitro.
资助项目	National Natural Science Foundation of China [81803781]; Wenzhou Basic Medical and Health Technology Project [Y20210024]; Key Laboratory of Novel Nuclide Technologies on Precision Diagnosis and Treatment & Clinical Transformation of Wenzhou City [2023HZSY0012]; Discipline Cluster of Oncology of Wenzhou Medical University [z1-2023007]
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
ISSN	0753-3322
EISSN	1950-6007
卷号	177
DOI	10.1016/j.biopha.2024.117110
页数	15
WOS类目	Medicine, Research & Experimental ; Pharmacology & Pharmacy
WOS研究方向	Research & Experimental Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:001271631800001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	39002439
SCOPUSEID	2-s2.0-85198063610
通讯作者地址	[Lin, Feng]Wenzhou Med Univ, Affiliated Hosp 1, Dept Gynecol, Wenzhou 325000, Zhejiang, Peoples R China. ; [Zheng, Xiaohui]Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Zhejiang, Peoples R China.
Scopus学科分类	Pharmacology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/216822
专题	附属第一医院药学院（分析测试中心）附属第一医院_妇科仁济学院
通讯作者	Zheng, Xiaohui; Lin, Feng
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 1, Dept Gynecol, Wenzhou 325000, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Zhejiang, Peoples R China; 3.Wenzhou Med Univ, Renji Coll, Wenzhou 325035, Zhejiang, Peoples R China
第一作者单位	附属第一医院; 妇科; 药学院（分析测试中心）
通讯作者单位	附属第一医院; 妇科; 药学院（分析测试中心）
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Chen, Kejie,Zhai, Yihui,Wang, Yuanqiu,et al. H2O2 promotes photodynamic efficacy of TMPyP4 against ovarian cancer in vitro by downregulating HIF-1α expression[J]. BIOMEDICINE & PHARMACOTHERAPY,2024,177.
APA	Chen, Kejie., Zhai, Yihui., Wang, Yuanqiu., Xu, Zichuang., Chen, Xiaojian., ... & Lin, Feng. (2024). H2O2 promotes photodynamic efficacy of TMPyP4 against ovarian cancer in vitro by downregulating HIF-1α expression. BIOMEDICINE & PHARMACOTHERAPY, 177.
MLA	Chen, Kejie,et al."H2O2 promotes photodynamic efficacy of TMPyP4 against ovarian cancer in vitro by downregulating HIF-1α expression".BIOMEDICINE & PHARMACOTHERAPY 177(2024).