Spermine oxidase regulates liver inflammation and fibrosis through β-catenin pathway

doi:10.1016/j.clinre.2024.102421

科研成果详情

题名	Spermine oxidase regulates liver inflammation and fibrosis through β-catenin pathway
作者	Hu, Tingting1; Tang, Wenqing 2; Hong, Wandong1; Huang, Qingke1; Sun, Xuecheng1; Wu, Wenzhi1; Zhang, Jie 3
发表日期	2024-08
发表期刊	CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Spermine oxidase Lipopolysaccharide Inflammation Liver cirrhosis
其他关键词	GASTRIC-CANCER RISK ; HEPATOCELLULAR-CARCINOMA ; POLYAMINE OXIDASE ; EPITHELIAL-CELLS ; HEPATITIS-C ; INJURY ; HOMEOSTASIS ; CIRRHOSIS ; PROTEINS ; DISEASE
摘要	Background: Spermine oxidase (SMOX), an inducible enzyme involved in the catabolic pathway of polyamine, was found to be upregulated in hepatocellular carcinoma and might be an important oncogene of it in our previous studies. This study attempted to further investigate its relationship with liver inflammation and fibrosis both in vitro and in vivo. Methods: The effect of SMOX inhibition on LPS-induced inflammatory response in mouse liver cell line AML12 was validated by using small interfering RNA or SMOX inhibitor MDL72527. Western blotting and immunofluorescence were utilized to verify whether LPS could induce beta-catenin to transfer into the nucleus and whether it could be reversed by interfering with the expression of SMOX or using SMOX inhibitor. Then, the SMOX inhibitor MDL72527 and SMOX knockout mice were used to verify the hypothesis above in vivo. Results: The expression of SMOX could be induced by LPS in AML12 cells. The inhibition of SMOX could inhibit LPS-induced inflammatory response in AML12 cells. LPS could induce beta-catenin transfer from cytoplasm to nucleus, while SMOX downregulation or inhibition could partially reverse this process. In vivo intervention with SMOX inhibitor MDL72527 or SMOX knockout mice could significantly improve the damage of liver function, reduce intrahepatic inflammation, inhibit the nuclear transfer of beta-catenin in liver tissue, and alleviate carbon tetrachloride-induced liver fibrosis in mice. Conclusion: SMOX can promote the inflammatory response and fibrosis of hepatocytes. It provides a new therapeutic strategy for hepatitis and liver fibrosis, inhibiting early liver cancer.
资助项目	Fund of Medicine and Technology project of Zhejiang province of China [2023RC206]
出版者	ELSEVIER MASSON, CORP OFF
ISSN	2210-7401
EISSN	2210-741X
卷号	48 期号:7
DOI	10.1016/j.clinre.2024.102421
页数	9
WOS类目	Gastroenterology & Hepatology
WOS研究方向	Gastroenterology & Hepatology
WOS记录号	WOS:001273431200001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	39002816
SCOPUSEID	2-s2.0-85198553422
通讯作者地址	[Zhang, Jie]Wenzhou Med Univ, Affiliated Hosp 1, Dept Otolaryngol, Nanbaixiang, Wenzhou 325000, Zhejiang, Peoples R China.
Scopus学科分类	Gastroenterology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/216770
专题	附属第一医院附属第一医院_耳鼻咽喉科附属第一医院_消化内科
通讯作者	Zhang, Jie
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 1, Dept Gastroenterol & Hepatol, Wenzhou 325000, Peoples R China; 2.Fudan Univ, Zhongshan Hosp, Shanghai Inst Liver Dis, Dept Gastroenterol & Hepatol, Shanghai 200032, Peoples R China; 3.Wenzhou Med Univ, Affiliated Hosp 1, Dept Otolaryngol, Nanbaixiang, Wenzhou 325000, Zhejiang, Peoples R China
第一作者单位	附属第一医院; 消化内科
通讯作者单位	附属第一医院; 耳鼻咽喉科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Hu, Tingting,Tang, Wenqing,Hong, Wandong,et al. Spermine oxidase regulates liver inflammation and fibrosis through β-catenin pathway[J]. CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY,2024,48(7).
APA	Hu, Tingting., Tang, Wenqing., Hong, Wandong., Huang, Qingke., Sun, Xuecheng., ... & Zhang, Jie. (2024). Spermine oxidase regulates liver inflammation and fibrosis through β-catenin pathway. CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY, 48(7).
MLA	Hu, Tingting,et al."Spermine oxidase regulates liver inflammation and fibrosis through β-catenin pathway".CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY 48.7(2024).