Inhibitory effects of parabens on human and rat 17β-hydroxysteroid dehydrogenase 1: Mechanisms of action and impact on hormone synthesis

doi:10.1016/j.tox.2024.153873

科研成果详情

题名	Inhibitory effects of parabens on human and rat 17β-hydroxysteroid dehydrogenase 1: Mechanisms of action and impact on hormone synthesis
作者	Chen, Zouqi 1,2; Gong, Chaochao 1,2; Tang, Yunbing 1,2; Zhu, Yang2,3,4,5; Wang, Shaowei1,2; Ge, Ren-shan1,2,3,4,5,6; Ying, Yingfen 1,2
发表日期	2024-08
发表期刊	TOXICOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	17 beta-HSD1 Parabens Docking analysis Estradiol Bivariate correlation
其他关键词	DATABASE
摘要	Parabens are commonly used preservatives in cosmetics, food, and pharmaceutical products. The objective of this study was to examine the effect of nine parabens on human and rat 17 beta- hydroxysteroid dehydrogenase 1 (17 beta- HSD1) in human placental and rat ovarian cytosols, as well as on estradiol synthesis in BeWo cells. The results showed that the IC 50 values for these compounds varied from methylparaben with the weakest inhibition (106.42 mu M) to hexylparaben with the strongest inhibition (2.05 mu M) on human 17 beta- HSD1. Mode action analysis revealed that these compounds acted as mixed inhibitors. For rats, the IC 50 values ranged from the weakest inhibition for methylparaben (no inhibition at 100 mu M) to the most potent inhibition for hexylparaben (0.87 mu M), and they functioned as mixed inhibitors. Docking analysis indicated that parabens bind to the region bridging the NADPH and steroid binding sites of human 17 beta- HSD1 and the NADPH binding site of rat 17 beta- HSD1. Bivariate correlation analysis demonstrated negative correlations between LogP, molecular weight, heavy atoms, and apolar desolvation energy, and the IC 50 values of these compounds. In conclusion, this study identified the inhibitory effects of parabens and their binding mechanisms on human and rat 17 beta- HSD1, as well as their impact on hormone synthesis.
资助项目	National Natural Science Foundation of China [82371610, 82301892]; Zhejiang Provincial Natural Science Fund [LY22H040010]; Scientific Research Center of Wenzhou Medical University
出版者	ELSEVIER IRELAND LTD
ISSN	0300-483X
EISSN	1879-3185
卷号	506
DOI	10.1016/j.tox.2024.153873
页数	12
WOS类目	Pharmacology & Pharmacy ; Toxicology
WOS研究方向	Pharmacology & Pharmacy ; Toxicology
WOS记录号	WOS:001271335000001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	38986729
SCOPUSEID	2-s2.0-85198303717
通讯作者地址	[Ge, Ren-shan]Second Affiliated Hosp, Dept Anesthesiol & Perioperat Med, Wenzhou 325000, Zhejiang, Peoples R China. ; [Ge, Ren-shan]Yuying Childrens Hosp, Wenzhou 325000, Zhejiang, Peoples R China. ; [Ying, Yingfen]Key Lab Struct Malformat Children Zhejiang Prov, Wenzhou 325000, Zhejiang, Peoples R China.
Scopus学科分类	Toxicology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/216733
专题	附属第二医院第二临床医学院、附属第二医院、育英儿童医院附属第二医院_麻醉科附属第二医院_麻醉重点学科实验室
通讯作者	Ge, Ren-shan; Ying, Yingfen
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 2, Dept Obstet & Gynecol, Wenzhou 325027, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Zhejiang, Peoples R China; 3.Wenzhou Med Univ, Key Lab Anesthesiol Zhejiang Prov, Wenzhou 325027, Zhejiang, Peoples R China; 4.Minist Educ, Key Lab Pediat Anesthesiol, Wenzhou 325027, Zhejiang, Peoples R China; 5.Wenzhou Med Univ, Affiliated Hosp 2, Dept Anesthesiol & Perioperat Med, Wenzhou 325027, Zhejiang, Peoples R China; 6.Key Lab Male Hlth & Environm Wenzhou, Wenzhou 325000, Zhejiang, Peoples R China
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Chen, Zouqi,Gong, Chaochao,Tang, Yunbing,et al. Inhibitory effects of parabens on human and rat 17β-hydroxysteroid dehydrogenase 1: Mechanisms of action and impact on hormone synthesis[J]. TOXICOLOGY,2024,506.
APA	Chen, Zouqi., Gong, Chaochao., Tang, Yunbing., Zhu, Yang., Wang, Shaowei., ... & Ying, Yingfen. (2024). Inhibitory effects of parabens on human and rat 17β-hydroxysteroid dehydrogenase 1: Mechanisms of action and impact on hormone synthesis. TOXICOLOGY, 506.
MLA	Chen, Zouqi,et al."Inhibitory effects of parabens on human and rat 17β-hydroxysteroid dehydrogenase 1: Mechanisms of action and impact on hormone synthesis".TOXICOLOGY 506(2024).