Mitochondrial biogenesis disorder and oxidative damage promote refractory apical periodontitis in rat and human

doi:10.1111/iej.14106

科研成果详情

题名	Mitochondrial biogenesis disorder and oxidative damage promote refractory apical periodontitis in rat and human
作者	Wang, Jun 1,2; Chen, Yuge1,3; Yuan, Huina1,4; Zhang, Xuejia1,3; Febbraio, Maria 3; Pan, Yihuai1,5; Huang, Shengbin1,2; Liu, Zhongfang 1,5
发表日期	2024-06-16
发表期刊	International Endodontic Journal 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	mitochondrial biogenesis oxidative stress refractory apical periodontitis
其他关键词	DYSFUNCTION ; ACTIVATION ; STRESS
摘要	Aim: To elucidate whether mitochondrial biogenesis disorder and damage from oxidative stress promote refractory apical periodontitis (RAP) in rat and human. Methodology: Twenty Enterococcus faecalis-induced RAPs were established in the maxillary first molars of male Wistar rats. Concurrently, 12 periapical lesion specimens from patients presenting with RAP were obtained by apicoectomy. Radiographic examination and histologic analysis were conducted to evaluate periapical bone tissue destruction and morphological changes. The expression of key regulators of mitochondrial biogenesis, PGC-1 alpha and Nrf2, were detected by immunohistochemistry and double immunofluorescence staining, Western blot and real-time PCR were also assayed. Mitochondrial ROS (mtROS) was identified by MitoSOX staining. Mitochondrial function was detected by the quantification of ATP production, mitochondrial DNA (mtDNA) copy number and activities of mitochondrial respiratory chain complexes. Furthermore, mitochondrial oxidative stress was evaluated by the determination of 3-nitrotyrosine (3-NT), 4-hydroxy-2-nonenal (4-HNE) and 8-hydroxy-deoxyguanosine (8-OHdG) expression levels, as well as malondialdehyde (MDA) expression and antioxidant capacity. Student's t-test was performed to determine significance between the groups; p < .05 was considered significant. Results: In the maxilla, significantly more bone resorption, greater number of periapical apoptotic cells and Tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells were observed in the RAP group compared with the control group (p < .01). PGC-1 alpha and Nrf2 were significantly reduced in rat and human RAP lesions compared to the control group (p < .01) at both the mRNA and protein levels. Double immunofluorescence analysis of PGC-1 alpha or Nrf2 with TOMM20 also indicated that mitochondrial biogenesis was impaired in RAP group (p < .01). Additionally, mitochondrial dysfunction was observed in RAP group, as reflected by increased mtROS, decreased ATP production, reduced mtDNA copy number and complexes of the mitochondrial respiratory chain. Finally, the expression levels of mitochondrial oxidative stress markers, 3-NT, 4-HNE and 8-OHdG, were significantly increased in the RAP group (p < .01). Consistent with this, systemic oxidative damage was also present in the progression of RAP, including increased MDA expression and decreased antioxidant activity (p < .01). Conclusions: Mitochondrial biogenesis disorder and damage from oxidative stress contribute to the development of RAP.
资助项目	Zhejiang Provincial Natural Science Foundation of China
出版者	WILEY
ISSN	0143-2885
EISSN	1365-2591
卷号	57 期号:9 页码:1326-1342
DOI	10.1111/iej.14106
页数	17
WOS类目	Dentistry, Oral Surgery & Medicine
WOS研究方向	Dentistry, Oral Surgery & Medicine
WOS记录号	WOS:001250191400001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	38881187
SCOPUSEID	2-s2.0-85196201218
通讯作者地址	[Pan, Yihuai;Huang, Shengbin;Liu, Zhongfang]Wenzhou Med Univ, Sch & Hosp Stomatol, 1288 Longyao Rd, Wenzhou 325000, Zhejiang, Peoples R China.
Scopus学科分类	Dentistry (all)
SCOPUS_ID	SCOPUS_ID:85196201218
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/215250
专题	口腔医学院、附属口腔医院口腔医学院、附属口腔医院_正畸科口腔医学院、附属口腔医院_口腔修复
通讯作者	Pan, Yihuai; Huang, Shengbin; Liu, Zhongfang
作者单位	1.Wenzhou Med Univ, Sch & Hosp Stomatol, 1288 Longyao Rd, Wenzhou 325000, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Sch & Hosp Stomatol, Dept Prosthodont, Wenzhou, Peoples R China; 3.Univ Alberta, Fac Med & Dent, Dept Dent, Edmonton, AB, Canada; 4.Wenzhou Med Univ, Sch & Hosp Stomatol, Dept Orthodont, Wenzhou, Peoples R China; 5.Wenzhou Med Univ, Sch & Hosp Stomatol, Dept Endodont, Wenzhou, Peoples R China
第一作者单位	口腔医学院、附属口腔医院; 口腔修复
通讯作者单位	口腔医学院、附属口腔医院
第一作者的第一单位	口腔医学院、附属口腔医院
推荐引用方式 GB/T 7714	Wang, Jun,Chen, Yuge,Yuan, Huina,et al. Mitochondrial biogenesis disorder and oxidative damage promote refractory apical periodontitis in rat and human[J]. International Endodontic Journal,2024,57(9):1326-1342.
APA	Wang, Jun., Chen, Yuge., Yuan, Huina., Zhang, Xuejia., Febbraio, Maria., ... & Liu, Zhongfang. (2024). Mitochondrial biogenesis disorder and oxidative damage promote refractory apical periodontitis in rat and human. International Endodontic Journal, 57(9), 1326-1342.
MLA	Wang, Jun,et al."Mitochondrial biogenesis disorder and oxidative damage promote refractory apical periodontitis in rat and human".International Endodontic Journal 57.9(2024):1326-1342.