题名 | Mitochondrial biogenesis disorder and oxidative damage promote refractory apical periodontitis in rat and human |
作者 | |
发表日期 | 2024-06-16 |
发表期刊 | International Endodontic Journal 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article ; Early Access |
关键词 | mitochondrial biogenesis oxidative stress refractory apical periodontitis |
其他关键词 | DYSFUNCTION ; ACTIVATION ; STRESS |
摘要 | Aim: To elucidate whether mitochondrial biogenesis disorder and damage from oxidative stress promote refractory apical periodontitis (RAP) in rat and human. Methodology: Twenty Enterococcus faecalis-induced RAPs were established in the maxillary first molars of male Wistar rats. Concurrently, 12 periapical lesion specimens from patients presenting with RAP were obtained by apicoectomy. Radiographic examination and histologic analysis were conducted to evaluate periapical bone tissue destruction and morphological changes. The expression of key regulators of mitochondrial biogenesis, PGC-1 alpha and Nrf2, were detected by immunohistochemistry and double immunofluorescence staining, Western blot and real-time PCR were also assayed. Mitochondrial ROS (mtROS) was identified by MitoSOX staining. Mitochondrial function was detected by the quantification of ATP production, mitochondrial DNA (mtDNA) copy number and activities of mitochondrial respiratory chain complexes. Furthermore, mitochondrial oxidative stress was evaluated by the determination of 3-nitrotyrosine (3-NT), 4-hydroxy-2-nonenal (4-HNE) and 8-hydroxy-deoxyguanosine (8-OHdG) expression levels, as well as malondialdehyde (MDA) expression and antioxidant capacity. Student's t-test was performed to determine significance between the groups; p < .05 was considered significant. Results: In the maxilla, significantly more bone resorption, greater number of periapical apoptotic cells and Tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells were observed in the RAP group compared with the control group (p < .01). PGC-1 alpha and Nrf2 were significantly reduced in rat and human RAP lesions compared to the control group (p < .01) at both the mRNA and protein levels. Double immunofluorescence analysis of PGC-1 alpha or Nrf2 with TOMM20 also indicated that mitochondrial biogenesis was impaired in RAP group (p < .01). Additionally, mitochondrial dysfunction was observed in RAP group, as reflected by increased mtROS, decreased ATP production, reduced mtDNA copy number and complexes of the mitochondrial respiratory chain. Finally, the expression levels of mitochondrial oxidative stress markers, 3-NT, 4-HNE and 8-OHdG, were significantly increased in the RAP group (p < .01). Consistent with this, systemic oxidative damage was also present in the progression of RAP, including increased MDA expression and decreased antioxidant activity (p < .01). Conclusions: Mitochondrial biogenesis disorder and damage from oxidative stress contribute to the development of RAP. |
资助项目 | Zhejiang Provincial Natural Science Foundation of China |
出版者 | WILEY |
ISSN | 0143-2885 |
EISSN | 1365-2591 |
卷号 | 57期号:9页码:1326-1342 |
DOI | 10.1111/iej.14106 |
页数 | 17 |
WOS类目 | Dentistry, Oral Surgery & Medicine |
WOS研究方向 | Dentistry, Oral Surgery & Medicine |
WOS记录号 | WOS:001250191400001 |
收录类别 | SCIE ; SCOPUS ; PUBMED |
URL | 查看原文 |
PubMed ID | 38881187 |
SCOPUSEID | 2-s2.0-85196201218 |
通讯作者地址 | [Pan, Yihuai;Huang, Shengbin;Liu, Zhongfang]Wenzhou Med Univ, Sch & Hosp Stomatol, 1288 Longyao Rd, Wenzhou 325000, Zhejiang, Peoples R China. |
Scopus学科分类 | Dentistry (all) |
SCOPUS_ID | SCOPUS_ID:85196201218 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/215250 |
专题 | 口腔医学院、附属口腔医院 口腔医学院、附属口腔医院_正畸科 口腔医学院、附属口腔医院_口腔修复 |
通讯作者 | Pan, Yihuai; Huang, Shengbin; Liu, Zhongfang |
作者单位 | 1.Wenzhou Med Univ, Sch & Hosp Stomatol, 1288 Longyao Rd, Wenzhou 325000, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Sch & Hosp Stomatol, Dept Prosthodont, Wenzhou, Peoples R China; 3.Univ Alberta, Fac Med & Dent, Dept Dent, Edmonton, AB, Canada; 4.Wenzhou Med Univ, Sch & Hosp Stomatol, Dept Orthodont, Wenzhou, Peoples R China; 5.Wenzhou Med Univ, Sch & Hosp Stomatol, Dept Endodont, Wenzhou, Peoples R China |
第一作者单位 | 口腔医学院、附属口腔医院; 口腔修复 |
通讯作者单位 | 口腔医学院、附属口腔医院 |
第一作者的第一单位 | 口腔医学院、附属口腔医院 |
推荐引用方式 GB/T 7714 | Wang, Jun,Chen, Yuge,Yuan, Huina,et al. Mitochondrial biogenesis disorder and oxidative damage promote refractory apical periodontitis in rat and human[J]. International Endodontic Journal,2024,57(9):1326-1342. |
APA | Wang, Jun., Chen, Yuge., Yuan, Huina., Zhang, Xuejia., Febbraio, Maria., ... & Liu, Zhongfang. (2024). Mitochondrial biogenesis disorder and oxidative damage promote refractory apical periodontitis in rat and human. International Endodontic Journal, 57(9), 1326-1342. |
MLA | Wang, Jun,et al."Mitochondrial biogenesis disorder and oxidative damage promote refractory apical periodontitis in rat and human".International Endodontic Journal 57.9(2024):1326-1342. |
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