Verapamil increases susceptibility of colistin-resistant Acinetobacter baumannii to colistin

doi:10.1016/j.ijantimicag.2024.107233

科研成果详情

题名	Verapamil increases susceptibility of colistin-resistant Acinetobacter baumannii to colistin
作者	Sichen Liu1; Huijing Zhou 1; Jingchun Kong2; Huanchang Chen1; Lei Chen 1; Miran Tang1; Beibei Zhou1; Mengxin Xu1; Lijiang Chen3; Tieli Zhou4
发表日期	2024-05-31
发表期刊	International journal of antimicrobial agents 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Acinetobacter baumannii antimicrobial synergy verapamil
其他关键词	MECHANISMS ; EFFICACY ; RATES
摘要	Acinetobacter baumannii, which is predominantly responsible for hospital-acquired infections, presents a tremendous clinical challenge due to its increasing antibiotic resistance to colistin (COL), a last-line antibiotic. As a result, the combination of antimicrobial and non-antimicrobial agents is emerging as a more popular treatment approach against infections caused by colistin-resistant (COL-R) A. baumannii. This study administered COL and verapamil (VER), i.e., an antihypertensive and antiarrhythmic agent. We found that the susceptibility of A. baumannii to COL was restored both in vitro and in vivo. Scanning electron microscope (SEM) and Crystal violet staining showed inhibition of the VER/COL combination on bacterial biofilm formation. Cytotoxicity assay and hemolysis test were used to confirm in vitro safety evaluation. Further experiments using propidium iodide (PI) staining revealed that the VER/COL combination improved the therapeutic efficacy of COL by modifying the permeability of bacterial membranes. As demonstrated by Reactive Oxygen Species (ROS) experiments, the drug combination caused the accumulation of bacterial ROS and their eventual death. Additionally, VER/COL treatment significantly reduced the efflux of Rhodamine 123 (Rh123). For the first time, this study identifies the anti-hypertensive drug VER as a COL potentiator against A. baumannii, providing a potential treatment approach against A. baumannii infections and improving patient outcomes.
资助项目	Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province[2022E10022];Wenzhou Medical University[SYXK 2021–0017]
出版者	Elsevier B.V.
ISSN	0924-8579
EISSN	1872-7913
卷号	64 期号:2
DOI	10.1016/j.ijantimicag.2024.107233
页数	9
WOS类目	Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
WOS研究方向	Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
WOS记录号	WOS:001287304200001
收录类别	PUBMED ; SCOPUS ; SCIE
URL	查看原文
PubMed ID	38824971
SCOPUSEID	2-s2.0-85197343969
通讯作者地址	[Chen, Lijiang]Department of Clinical Laboratory,The First Affiliated Hospital of Wenzhou Medical University,China,Wenzhou,325000,China
Scopus学科分类	Microbiology (medical);Infectious Diseases;Pharmacology (medical)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/215092
专题	附属第一医院检验医学院（生命科学学院、生物学实验教学中心）第一临床医学院（信息与工程学院）、附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_临床检验诊断学
作者单位	1.Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University; Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province.; 2.School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China.; 3.Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University; Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province. Electronic address: wyychenlijiang@163.com.; 4.Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University; Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province. Electronic address: wyztli@163.com.
第一作者单位	附属第一医院; 临床检验诊断学; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 临床检验诊断学; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Sichen Liu,Huijing Zhou,Jingchun Kong,et al. Verapamil increases susceptibility of colistin-resistant Acinetobacter baumannii to colistin[J]. International journal of antimicrobial agents,2024,64(2).
APA	Sichen Liu., Huijing Zhou., Jingchun Kong., Huanchang Chen., Lei Chen., ... & Tieli Zhou. (2024). Verapamil increases susceptibility of colistin-resistant Acinetobacter baumannii to colistin. International journal of antimicrobial agents, 64(2).
MLA	Sichen Liu,et al."Verapamil increases susceptibility of colistin-resistant Acinetobacter baumannii to colistin".International journal of antimicrobial agents 64.2(2024).