Quorum sensing gene lasR promotes phage vB_Pae_PLY infection in Pseudomonas aeruginosa

doi:10.1186/s12866-024-03349-7

科研成果详情

题名	Quorum sensing gene lasR promotes phage vB_Pae_PLY infection in Pseudomonas aeruginosa
作者	Liu, Yan; Yao, Zhuocheng; Mao, Zhenzhi; Tang, Miran; Chen, Huanchang; Qian, Changrui; Zeng, Weiliang; Zhou, Tieli; Wu, Qing
发表日期	2024-12
发表期刊	BMC Microbiology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	lasR Phage infection Phage receptor Pseudomonas aeruginosa Quorum sensing
其他关键词	LIPOPOLYSACCHARIDE CORE ; RESISTANCE ; IDENTIFICATION ; RECEPTOR ; VIRUSES ; GENOME
摘要	Background: Quorum sensing (QS) is a cell density-based intercellular communication system that controls virulence gene expression and biofilm formation. In Pseudomonas aeruginosa (P. aeruginosa), the LasR system sits at the top of the QS hierarchy and coordinates the expression of a series of important traits. However, the role of lasR in phage infection remains unclear. This study aims to investigate the role of lasR QS in phage infection. Methods: The P. aeruginosa phage was isolated from sewage, and its biological characteristics and whole genome were analyzed. The adsorption receptor was identified via a phage adsorption assay. Following lasR gene knockout, the adsorption rate and bactericidal activity of phage were analyzed. Finally, real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to explore how lasR promoting phage infection. Results: The lytic phage vB_Pae_PLY was isolated and lipopolysaccharide (LPS) was identified as its adsorption receptor. The adsorption rate and bactericidal activity of vB_Pae_PLY were reduced after lasR knockout. RT-qPCR results showed that the expression of galU, a key gene involved in LPS synthesis, was down-regulated, and several genes related to type IV pili (T4P) were also down-regulated in the lasR mutant PaΔlasR. Conclusions: The study showed that QS lasR may promote phage vB_Pae_PLY infection by involving in the synthesis of LPS and T4P. This study provides an example of QS in promoting phage infection and deepens the understanding of phage-bacteria interactions.
资助项目	the research grants from the National Natural Science Foundation of China[no. 82172328];the Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province[2022E10022];the Health Department of Zhejiang Province of the People's Republic of China[2022KY895]
出版者	BioMed Central Ltd
ISSN	1471-2180
卷号	24 期号:1
DOI	10.1186/s12866-024-03349-7
页数	12
WOS类目	Microbiology
WOS研究方向	Microbiology
WOS记录号	WOS:001243759800001
收录类别	SCOPUS ; PUBMED ; SCIE
URL	查看原文
PubMed ID	38858621
SCOPUSEID	2-s2.0-85195625530
通讯作者地址	[Zhou, Tieli]Department of Clinical Laboratory,Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China ; [Wu, Qing]Department of Clinical Laboratory,Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Microbiology;Microbiology (medical)
SCOPUS_ID	SCOPUS_ID:85195625530
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/215021
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_临床检验诊断学
通讯作者	Zhou, Tieli; Wu, Qing
作者单位	Department of Clinical Laboratory,Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	附属第一医院; 临床检验诊断学; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 临床检验诊断学; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Liu, Yan,Yao, Zhuocheng,Mao, Zhenzhi,et al. Quorum sensing gene lasR promotes phage vB_Pae_PLY infection in Pseudomonas aeruginosa[J]. BMC Microbiology,2024,24(1).
APA	Liu, Yan., Yao, Zhuocheng., Mao, Zhenzhi., Tang, Miran., Chen, Huanchang., ... & Wu, Qing. (2024). Quorum sensing gene lasR promotes phage vB_Pae_PLY infection in Pseudomonas aeruginosa. BMC Microbiology, 24(1).
MLA	Liu, Yan,et al."Quorum sensing gene lasR promotes phage vB_Pae_PLY infection in Pseudomonas aeruginosa".BMC Microbiology 24.1(2024).