Forensic Application of SiFaSTR TM 23plex DNA ID System in Han Population of Eastern China

doi:10.3969/j.issn.1004-5619.2018.02.003

科研成果详情

题名	Forensic Application of SiFaSTR TM 23plex DNA ID System in Han Population of Eastern China
其他题名	SiFaSTR TM 23plex DNA 身份鉴定系统在华东汉族人群中的法医学应用
作者	Bao, Yun 1,2; Sheng, Xiang 2,3; Zhang, Jia-Shuo 2,3; Li, Min 2,4; Li, Ya-Nan 1,2; Xu, Qian-Nan 2,4; Li, Cheng-Tao 2; Chen, Li-Qin 1
发表日期	2018-04
发表期刊	Journal of Forensic Medicine 影响因子和分区
语种	中文
原始文献类型	Article
关键词	DNA mutational analysis Eastern China Forensic genetics Han nationality Polymorphism, genetic Short tandem repeat
摘要	Objective: To investigate the genetic polymorphism of 21 autosomal STR loci and DYS391 locus of SiFaSTRTM 23plex DNA ID system in Han population of eastern China and to evaluate its application value in forensic science. Methods: Typing test of 2 000 unrelated individuals was performed using SiFaSTRTM 23plex DNA ID system. The population genetic parameters of STR loci were statistically analysed. A total of 3 198 parentage confirmed cases were detected with that system and the mutation conditions were observed in 21 autosomal STR loci. Results: All the 21 autosomal STR loci showed no significant departure from Hardy-Weinberg equilibrium (P>0.05). The Ho ranged from 0.617 5 to 0.927 0. The DP ranged from 0.796 4 to 0.986 9, as well as the PIC distributed from 0.561 1 to 0.912 3. The CDP was 0.999 999 999 999 999. The CPEduo was 0.999 997 431 701 961, while CPEtrio was 0.999 999 999 654 865. Five alleles were detected in DYS391 locus, with the allele frequency from 0.004 0 to 0.729 0, and GD was 0.418 9. Except D13S317 and D10S1248, seventy-six mutation events were observed at the rest nineteen autosomal STR loci. Among them, seventy-five (98.68%) were one step mutation, and only one (1.32%) was three steps mutation. The mutation rate ranged from 0.246 5×10-3 to 2.711 4×10-3, and the averaged mutation rate was 0.892 1×10-3 (95% CI: 0.70×10-3-1.10×10-3). In 33 trio mutation cases, the proportion of the paternal mutation and the maternal mutation was 2.09 :1. Conclusion: The involved STRs are highly polymorphic in Eastern Han population with acceptable mutation rates by the SiFaSTRTM 23plex DNA ID system, which is suitable for paternity testing and individual identification.
出版者	Journal of Forensic Medicinefyxzz@126.com
ISSN	1004-5619
卷号	34 期号:2 页码:120-125
DOI	10.3969/j.issn.1004-5619.2018.02.003
收录类别	SCOPUS
URL	查看原文
PubMed ID	29923374
SCOPUSEID	2-s2.0-85053772841
通讯作者地址	[Chen, Li-Qin]School of Basic Medicine,Inner Mongolia Medical University,Hohhot,010030,China
Scopus学科分类	Pathology and Forensic Medicine
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/214176
专题	基础医学院（机能实验教学中心）_法医学系
通讯作者	Chen, Li-Qin
作者单位	1.School of Basic Medicine,Inner Mongolia Medical University,Hohhot,010030,China; 2.Shanghai Key Laboratory of Forensic Medicine,Shanghai Forensic Service Platform,Academy of Forensic Science,Shanghai,200063,China; 3.Department of Forensic Medicine,Medical College of Soochow University,Suzhou,215123,China; 4.Department of Forensic Medicine,Wenzhou Medical University,Wenzhou,325035,China
推荐引用方式 GB/T 7714	Bao, Yun,Sheng, Xiang,Zhang, Jia-Shuo,et al. Forensic Application of SiFaSTR TM 23plex DNA ID System in Han Population of Eastern China[J]. Journal of Forensic Medicine,2018,34(2):120-125.
APA	Bao, Yun., Sheng, Xiang., Zhang, Jia-Shuo., Li, Min., Li, Ya-Nan., ... & Chen, Li-Qin. (2018). Forensic Application of SiFaSTR TM 23plex DNA ID System in Han Population of Eastern China. Journal of Forensic Medicine, 34(2), 120-125.
MLA	Bao, Yun,et al."Forensic Application of SiFaSTR TM 23plex DNA ID System in Han Population of Eastern China".Journal of Forensic Medicine 34.2(2018):120-125.