Primary Human Leukocyte Subsets Differentially Express Vaccinia Virus Receptors Enriched in Lipid Rafts

doi:10.1128/JVI.01545-13

科研成果详情

题名	Primary Human Leukocyte Subsets Differentially Express Vaccinia Virus Receptors Enriched in Lipid Rafts
作者	Byrd, Daniel 1; Amet, Tohti 1; Hu, Ningjie 2; Lan, Jie 1; Hu, Sishun 1,3; Yu, Qigui 1,4
发表日期	2013-08
发表期刊	JOURNAL OF VIROLOGY 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	TARGETED ONCOLYTIC POXVIRUS ; HEMATOPOIETIC-CELLS ; T-CELLS ; TROPISM ; PROTEIN ; POLARIZATION ; INFECTION ; MEMBRANE ; ENTRY ; FLOTILLINS
摘要	Poxviruses, including vaccinia virus (VV) and canarypox virus (ALVAC), do not indiscriminately infect all cell types of the primary human leukocytes (PHLs) that they encounter but instead demonstrate an extremely strong bias toward infection of monocytes and monocyte lineage cells. We studied the specific molecular events that determine the VV tropism for major PHL subsets including monocytes, B cells, neutrophils, NK cells, and T cells. We found that VV exhibited an extremely strong bias of cell surface protein-dependent binding to monocytes, B cells, and activated T cells to a similar degree and to neutrophils to a much lesser extent. Resting T cells and resting NK cells exhibited only trace amounts of VV binding. Activated T cells, however, became permissive to VV binding, infection, and replication, while activated NK cells still resisted VV binding. VV binding strongly colocalized with lipid rafts on the surfaces of all VV binding-susceptible PHL subsets, even when lipid rafts were relocated to cell uropods upon cell polarization. Immunosera raised against detergent-resistant membranes (DRMs) from monocytes or activated T cells, but not resting T cells, effectively cross-blocked VV binding to and infection of PHL subsets. CD29 and CD98, two lipid raft-associated membrane proteins that had been found to be important for VV entry into HeLa cells, had no effect on VV binding to and infection of primary activated T cells. Our data indicate that PHL subsets express VV protein receptors enriched in lipid rafts and that receptors are cross-presented on all susceptible PHLs.
资助项目	Grand Challenges Explorations (GCE) Phase II grant through the Bill & Melinda Gates Foundation [OPP1035237]; NIH [1R21AI104268, T32 AI060519]; Showalter Research Trust Fund; National Natural Science Foundation of Zhejiang Province [Y2110608]; Research Facilities Improvement Program from the National Center for Research Resources, NIH [C06 RR015481-01]
出版者	AMER SOC MICROBIOLOGY
ISSN	0022-538X
EISSN	0022-538X
卷号	87 期号:16 页码:9301-9312
DOI	10.1128/JVI.01545-13
页数	12
WOS类目	Virology
WOS研究方向	Virology
WOS记录号	WOS:000322535600044
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	237852
SCOPUSEID	2-s2.0-84881260754
通讯作者地址	[Yu, Qigui]Department of Microbiology and Immunology and Center for AIDS Research,Indiana University School of Medicine,United States
Scopus学科分类	Microbiology;Immunology;Insect Science;Virology
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/213300
专题	检验医学院（生命科学学院、生物学实验教学中心）_模式生物技术与应用重点实验室
通讯作者	Yu, Qigui
作者单位	1.Department of Microbiology and Immunology and Center for AIDS Research,Indiana University School of Medicine,United States; 2.Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms,Wenzhou Medical College,China; 3.College of Veterinary Medicine,Huazhong Agricultural University,China; 4.Division of Infectious Diseases,Department of Medicine,Indiana University School of Medicine,United States
推荐引用方式 GB/T 7714	Byrd, Daniel,Amet, Tohti,Hu, Ningjie,et al. Primary Human Leukocyte Subsets Differentially Express Vaccinia Virus Receptors Enriched in Lipid Rafts[J]. JOURNAL OF VIROLOGY,2013,87(16):9301-9312.
APA	Byrd, Daniel, Amet, Tohti, Hu, Ningjie, Lan, Jie, Hu, Sishun, & Yu, Qigui. (2013). Primary Human Leukocyte Subsets Differentially Express Vaccinia Virus Receptors Enriched in Lipid Rafts. JOURNAL OF VIROLOGY, 87(16), 9301-9312.
MLA	Byrd, Daniel,et al."Primary Human Leukocyte Subsets Differentially Express Vaccinia Virus Receptors Enriched in Lipid Rafts".JOURNAL OF VIROLOGY 87.16(2013):9301-9312.