基质血管片段通过ANG-1/Tie-2信号通路促进移植脂肪血管再生和存活

doi:10.3969/j.issn.2095-9400.2024.04.002

科研成果详情

题名	基质血管片段通过ANG-1/Tie-2信号通路促进移植脂肪血管再生和存活
其他题名	Stromal vascular fraction promotes angiogenesis and fat graft survival in mice through the ANG-1/Tie-2 signaling pathway
作者	倪彬婷1; 何玉仓1; 李磊2; 徐金钰2; 柳朝阳2; 李力群1
发表日期	2024-04-25
发表期刊	温州医科大学学报影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	自体脂肪移植基质血管碎片血管生成素-1 酪氨酸激酶受体-2
其他关键词	autologous fat transplantation ; stromal vascular fraction ; angiopoietin-1 ; tyrosine kinase receptors-2
摘要	目的：探讨基质血管片段（SVF）促进脂肪移植后的新血管形成的机制。方法：设计裸鼠的脂肪移植模型，设置对照组、SVFs组及酪氨酸激酶抑制剂（TKI）组3组脂肪组织移植动物模型组，采用大体称重、HE染色、Masson染色、Western blot法和免疫荧光染色等方法检测各实验组体质量、病理学表现、胶原沉积情况及各组ANG-1、p-Tie-2、CD31、BAX及BCL-2的蛋白表达情况并做统计学分析，研究SVFs对脂肪成活的影响。结果：SVFs组的移植物体质量明显高于对照组（P<0.001）。在给予TKI后，SVFs对移植物脂肪体质量的改善被逆转，TKI组移植物体质量明显小于SVFs组（P<0.001）;SVFs组脂肪细胞周围胶原沉积明显减少，TKI组脂肪组织变得碎片化和不完整。SVFs组较对照组ANG-1和p-Tie-2的表达水平上调，CD31蛋白表达显著增加（P<0.05）;TKI给药后，TKI组较SVFs组p-Tie-2和CD31蛋白表达水平下降（P<0.05）。SVFs组较对照组BAX表达降低，BCL-2表达升高（P<0.05）;TKI给药后，TKI组较SVFs组BAX表达升高，BCL-2表达降低（P<0.05）。结论：SVFs可以通过ANG-1/Tie-2信号通路促进血管生成，抑制细胞凋亡，从而提高移植脂肪的存活。
其他摘要	Objective:To investigate the mechanism of new blood vessel formation induced by stromal vascular fragment(SVF)after fat transplantation.Methods:We designed a fat transplantation model of nude mice,and set fat tissue transplantation animal model groups of control group,SVFs group and tyrosine kinase inhibitor(TKI)group.we used these methods of gross weighing,HE staining,Masson staining,Western blot and immunofluorescence staining to detect body mass,pathological manifestations,collagen deposition and protein expression of ANG-1,p-Tie-2,CD31,BAX and BCL-2 in each experimental group and made the statistical analysis to study the effect of SVFs on fat survival.Results:The quality of transplanted objects in SVFs group was significantly higher than that in control group(P＜0.001).After TKI administration,the effect of SVFs on the fat mass of grafts was reversed,and the weight of grafts in TKI group was significantly lower than that in SVFs group(P＜0.001).The collagen deposition around adipocytes in the SVFs group was significantly reduced,and the adipocytes in the TKI group became fragmented and incomplete.Compared with the control group,the expression levels of ANG-1 and p-Tie-2 in SVFs group were up-regulated,and the expression levels of CD31 protein were significantly increased(P＜0.05).After TKI administration,the expression levels of p-Tie-2 and CD31 protein in TKI group were decreased compared with SVFs group(P＜0.05).Compared with the control group,the expression of BAX in SVFs group was decreased and the expression of BCL-2 was increased(P＜0.05).The expression of BAX in TKI group was increased and the expression of BCL-2 was decreased that compared with the SVFs group(P＜0.05).Conclusion:SVFs can promote angiogenesis and inhibit apoptosis through ANG-l/Tie-2 signaling pathway,thereby improving the survival of transplanted fat.
资助项目	温州市基础性科研项目（Y2020953）;
ISSN	2095-9400
卷号	54 期号:04 页码:266-273
DOI	10.3969/j.issn.2095-9400.2024.04.002
页数	8
收录类别	CNKI ; 万方 ; 维普
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引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/212972
专题	附属第一医院第二临床医学院、附属第二医院、育英儿童医院
通讯作者	李力群
作者单位	1.温州医科大学附属第一医院整形外科; 2.温州医科大学第二临床医学院
第一作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	倪彬婷,何玉仓,李磊,等. 基质血管片段通过ANG-1/Tie-2信号通路促进移植脂肪血管再生和存活[J]. 温州医科大学学报,2024,54(04):266-273.
APA	倪彬婷, 何玉仓, 李磊, 徐金钰, 柳朝阳, & 李力群. (2024). 基质血管片段通过ANG-1/Tie-2信号通路促进移植脂肪血管再生和存活. 温州医科大学学报, 54(04), 266-273.
MLA	倪彬婷,et al."基质血管片段通过ANG-1/Tie-2信号通路促进移植脂肪血管再生和存活".温州医科大学学报 54.04(2024):266-273.