Exploring the pharmacological mechanism of Glycyrrhiza uralensis against KOA through integrating network pharmacology and experimental assessment

doi:10.1111/jcmm.18319

科研成果详情

题名	Exploring the pharmacological mechanism of Glycyrrhiza uralensis against KOA through integrating network pharmacology and experimental assessment
作者	Xu, Jianbo 1,2,3; Sun, Qi 4; Qiu, Min 1,2; Wu, Yungang5; Cheng, Liangyan 1,2; Jiang, Nanwan 6; Zhang, Ruogu 1,2,7; Chen, Jiali 1,2,7; Yuan, Wenhua 1,2,7; Jin, Hongting 1,2,7; Wang, Weidong 8; Cai, Yunhuo 9; Zhang, Chunchun 1,2; Wang, Pinger 1,2,7
发表日期	2024-05
发表期刊	Journal of cellular and molecular medicine 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Glycyrrhiza uralensis Fisch. inflammatory response knee osteoarthritis molecular docking network pharmacology
其他关键词	OSTEOARTHRITIS
摘要	Knee osteoarthritis (KOA), a major health and economic problem facing older adults worldwide, is a degenerative joint disease. Glycyrrhiza uralensis Fisch. (GC) plays an integral role in many classic Chinese medicine prescriptions for treating knee osteoarthritis. Still, the role of GC in treating KOA is unclear. To explore the pharmacological mechanism of GC against KOA, UPLC-Q-TOF/MS was conducted to detect the main compounds in GC. The therapeutic effect of GC on DMM-induced osteoarthritic mice was assessed by histomorphology, μCT, behavioural tests, and immunohistochemical staining. Network pharmacology and molecular docking were used to predict the potential targets of GC against KOA. The predicted results were verified by immunohistochemical staining Animal experiments showed that GC had a protective effect on DMM-induced KOA, mainly in the improvement of movement disorders, subchondral bone sclerosis and cartilage damage. A variety of flavonoids and triterpenoids were detected in GC via UPLC-Q-TOF/MS, such as Naringenin. Seven core targets (JUN, MAPK3, MAPK1, AKT1, TP53, RELA and STAT3) and three main pathways (IL-17, NF-κB and TNF signalling pathways) were discovered through network pharmacology analysis that closely related to inflammatory response. Interestingly, molecular docking results showed that the active ingredient Naringenin had a good binding effect on anti-inflammatory-related proteins. In the verification experiment, after the intervention of GC, the expression levels of pp65 and F4/80 inflammatory indicators in the knee joint of KOA model mice were significantly downregulated. GC could improve the inflammatory environment in DMM-induced osteoarthritic mice thus alleviating the physiological structure and dysfunction of the knee joint. GC might play an important role in the treatment of knee osteoarthritis.
资助项目	Zhejiang Provincial Natural Science Foundation of China[LY22H270005];the Research Project of Zhejiang Chinese Medical University[2022JKZKTS32];National Natural Science Foundation of China[82074457];National Natural Science Foundation of China[82104891];National Natural Science Foundation of China[82274550];the State Administration of Traditional Chinese Medicine of Zhejiang Province[2021ZZ014];the State Administration of Traditional Chinese Medicine of Zhejiang Province[2024ZL052];Zhejiang Provincial Natural Science Fundfor Distinguished Young Scholar of China[LR23H270001]
出版者	WILEY
ISSN	1582-1838
EISSN	1582-4934
卷号	28 期号:9
DOI	10.1111/jcmm.18319
页数	11
WOS类目	Cell Biology ; Medicine, Research & Experimental
WOS研究方向	Cell Biology ; Research & Experimental Medicine
WOS记录号	WOS:001226345700002
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	38742846
SCOPUSEID	2-s2.0-85193071455
通讯作者地址	[Zhang, Chunchun]College of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Zhejiang,Hangzhou,310053,China ; [Wang, Pinger]Institute of Orthopadics and Traumatology,The First Affiliated Hospital of Zhejiang Chinese Medical University,Zhejiang,China ; [Cai, Yunhuo]Department of the Orthopedic Surgery,The Third Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
Scopus学科分类	Molecular Medicine;Cell Biology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/212873
专题	附属第一医院其他_附属萧山医院（杭州市萧山区第一人民医院）
通讯作者	Cai, Yunhuo; Zhang, Chunchun; Wang, Pinger
作者单位	1.Institute of Orthopedics and Traumatology,The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine),Hangzhou,China; 2.College of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou,China; 3.The First People's Hospital of Xiaoshan District,Xiaoshan Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 4.Department of Orthopedic Joint Surgery,Hangzhou Fuyang Hospital of TCM Orthopaedics and Traumatology,Hangzhou,China; 5.Department of the Orthopedics of TCM,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 6.Hangzhou Yiyuan Pharmaceutical Technology Co.,Ltd.,Hangzhou,China; 7.The First College of Clinical Medicine,Zhejiang Chinese Medical University,Hangzhou,China; 8.Department of the Orthopedic Surgery,The Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China; 9.Department of the Orthopedic Surgery,The Third Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
第一作者单位	温州医科大学
推荐引用方式 GB/T 7714	Xu, Jianbo,Sun, Qi,Qiu, Min,et al. Exploring the pharmacological mechanism of Glycyrrhiza uralensis against KOA through integrating network pharmacology and experimental assessment[J]. Journal of cellular and molecular medicine,2024,28(9).
APA	Xu, Jianbo., Sun, Qi., Qiu, Min., Wu, Yungang., Cheng, Liangyan., ... & Wang, Pinger. (2024). Exploring the pharmacological mechanism of Glycyrrhiza uralensis against KOA through integrating network pharmacology and experimental assessment. Journal of cellular and molecular medicine, 28(9).
MLA	Xu, Jianbo,et al."Exploring the pharmacological mechanism of Glycyrrhiza uralensis against KOA through integrating network pharmacology and experimental assessment".Journal of cellular and molecular medicine 28.9(2024).