科研成果详情

题名Discordant results between Xpert MTB/RIF assay and Bactec MGIT 960 culture system regarding the detection of rifampin-resistant Mycobacterium tuberculosis isolates in Wenzhou, China
作者
发表日期2024-05-13
发表期刊Microbiology spectrum   影响因子和分区
语种英语
原始文献类型Journal Article
关键词Xpert MTB/RIF assay, Bactec MGIT 960 borderline RIF resistance drug-resistant tuberculosis rpoB mutations
其他关键词RPOB MUTATIONS ; ACCURACY
摘要This study aimed to assess the possible causes of discordant results between Xpert MTB/RIF (Xpert) and Bactec MGIT 960 Culture System (MGIT960) regarding rifampicin (RIF) susceptibility in Mycobacterium tuberculosis. Patients with previous RIF-resistant tuberculosis who were admitted to Wenzhou Central Hospital from January 2020 to December 2022 were enrolled. The isolates obtained from these patients were subjected to RIF susceptibility tests using Xpert and MGIT960, and the minimum inhibitory concentration (MIC) of RIF was determined by the MYCOTB MIC plate test. Additionally, molecular docking and molecular dynamics (MD) simulations were performed to evaluate the binding efficacy of rpoB and RIF based on rpoB mutations detected in the isolates with discordant RIF susceptibility results. A total of 28 isolates with discordant RIF susceptibility test results were detected, 15 of them were RIF susceptible with MICs ≤ 0.5 µg/mL. Twelve out of 15 isolates contained borderline RIF resistance-associated mutations [L430P (n = 6), H445N (n = 6)], 1 isolate had D435Y and Q429H double mutation, and the remaining 2 isolates had a silent (Q432Q) mutation. Compared with the affinity of RIF toward the wild type (WT) (-45.83 kcal/mol) by MD, its affinity toward L452P (-55.52 kcal/mol), D435Y (-47.39 kcal/mol), L430P (approximately -69.72 kcal/mol), H445N (-49.53 kcal/mol), and Q429H (-55.67 kcal/mol) increased. Borderline RIF resistance-associated mutations were the main cause for the discordant RIF susceptibility results between Xpert and MGIT960, and the mechanisms of the resistance need further investigated.IMPORTANCEThis study is aimed at assessing discordant results between Xpert MTB/RIF (Xpert) assay and Bactec MGIT 960 Culture System (MGIT960) regarding the detection of rifampicin (RIF)-resistant Mycobacterium tuberculosis isolates in Wenzhou, China. The discordant results of RIF between these two assays were mainly caused by borderline RIF resistance-associated mutations, subsequently by silent mutations of rpoB. Borderline RIF resistance- associated mutations detected in our study were demonstrated to not be affected by the affinity of rpoB and RIF by molecular dynamics, and the mechanism of resistance was needed to be clarified. For the discordant results of RIF by Xpert and MGIT960 that occurred, rpoB DNA sequencing was recommended to investigate its association with resistance to RIF
资助项目Basic Public Welfare Research Project of Zhejiang Province [LGF20H010003]; Wenzhou Science and Technology Plan Project [Y20220030]; Shangda Translational Medicine Foundation of Wenzhou Research Institute of Shanghai University [SDTMF2022EP02]
出版者AMER SOC MICROBIOLOGY
ISSN2165-0497
卷号12期号:6
DOI10.1128/spectrum.03859-23
页数11
WOS类目Microbiology
WOS研究方向Microbiology
WOS记录号WOS:001220688600001
收录类别PUBMED ; SCIE ; SCOPUS
URL查看原文
PubMed ID38738892
SCOPUSEID2-s2.0-85195253959
通讯作者地址[He, Guiqing;Jiang, Xiangao]Wenzhou Med Univ, Affiliated Hosp Shanghai Univ 2, Dingli Clin Coll, Dept Infect Dis,Wenzhou Cent Hosp, Wenzhou, Peoples R China. ; [He, Guiqing]Wenzhou Med Univ, Affiliated Hosp Shanghai Univ 2, Dingli Clin Coll, Wenzhou Cent Hosp,Lab Infect Dis, Wenzhou, Peoples R China. ; [Huang, Hairong;Yu, Xia]Capital Med Univ, Beijing Chest Hosp, Natl Clin Lab TB, Beijing Key Lab Drug Resistant TB, Beijing, Peoples R China.
Scopus学科分类Physiology;Ecology;Immunology and Microbiology (all);Genetics;Microbiology (medical);Cell Biology;Infectious Diseases
引用统计
文献类型期刊论文
条目标识符https://kms.wmu.edu.cn/handle/3ETUA0LF/212867
专题附属第二医院
其他_定理临床学院(温州市中心医院)
通讯作者He, Guiqing; Huang, Hairong; Jiang, Xiangao; Yu, Xia
作者单位
1.Wenzhou Med Univ, Affiliated Hosp Shanghai Univ 2, Dingli Clin Coll, Dept Infect Dis,Wenzhou Cent Hosp, Wenzhou, Peoples R China;
2.Wenzhou Med Univ, Affiliated Hosp Shanghai Univ 2, Dingli Clin Coll, Wenzhou Cent Hosp,Lab Infect Dis, Wenzhou, Peoples R China;
3.Capital Med Univ, Beijing Chest Hosp, Natl Clin Lab TB, Beijing Key Lab Drug Resistant TB, Beijing, Peoples R China;
4.Wenzhou Med Univ, Affiliated Hosp Shanghai Univ 2, Dingli Clin Coll, Wenzhou Cent Hosp,Dept Clin Lab Med, Wenzhou, Peoples R China;
5.Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing, Peoples R China
第一作者单位定理临床学院(温州市中心医院)
通讯作者单位定理临床学院(温州市中心医院)
第一作者的第一单位定理临床学院(温州市中心医院)
推荐引用方式
GB/T 7714
He, Guiqing,Zheng, Qingyong,Wu, Jing,et al. Discordant results between Xpert MTB/RIF assay and Bactec MGIT 960 culture system regarding the detection of rifampin-resistant Mycobacterium tuberculosis isolates in Wenzhou, China[J]. Microbiology spectrum,2024,12(6).
APA He, Guiqing., Zheng, Qingyong., Wu, Jing., Wu, Lianpeng., Geng, Zhi., ... & Yu, Xia. (2024). Discordant results between Xpert MTB/RIF assay and Bactec MGIT 960 culture system regarding the detection of rifampin-resistant Mycobacterium tuberculosis isolates in Wenzhou, China. Microbiology spectrum, 12(6).
MLA He, Guiqing,et al."Discordant results between Xpert MTB/RIF assay and Bactec MGIT 960 culture system regarding the detection of rifampin-resistant Mycobacterium tuberculosis isolates in Wenzhou, China".Microbiology spectrum 12.6(2024).

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