Liquiritin reduces chondrocyte apoptosis through P53/PUMA signaling pathway to alleviate osteoarthritis

doi:10.1016/j.lfs.2024.122536

科研成果详情

题名	Liquiritin reduces chondrocyte apoptosis through P53/PUMA signaling pathway to alleviate osteoarthritis
作者	Qiu, Min 1,2; Cheng, Liangyan 1,2; Xu, Jianbo 1,4; Jin, Minwei 5; Yuan, Wenhua 1,3; Ge, Qinwen 1,3; Zou, Kaiao 1,3; Chen, Jiali 1,3; Huang, Yuliang 5; Li, Ju 1; Zhu, Liming4; Xu, Bing 6; Zhang, Chunchun 1,2; Jin, Hongting 1,3; Wang, Pinger 1,3
发表日期	2024-04-15
发表期刊	Life sciences 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Apoptosis Liquiritin Osteoarthritis P53/PUMA
其他关键词	KNEE OSTEOARTHRITIS ; BH3-ONLY PROTEINS ; CELL-DEATH ; ALPHA ; PUMA
摘要	The main pathological features of osteoarthritis (OA) include the degeneration of articular cartilage and a decrease in matrix synthesis. Chondrocytes, which contribute to matrix synthesis, play a crucial role in the development of OA. Liquiritin, an effective ingredient extracted from Glycyrrhiza uralensis Fisch., has been used for over 1000 years to treat OA. This study aims to investigate the impact of liquiritin on OA and its underlying mechanism., Gait and hot plate tests assessed mouse behavior, while Micro-CT and ABH/OG staining observed joint morphological changes. The TUNEL kit detected chondrocyte apoptosis. Western blot and immunofluorescence techniques determined the expression levels of cartilage metabolism markers COL2 and MMP13, as well as apoptosis markers caspase3, bcl2, P53, and PUMA. KEGG analysis and molecular docking technology were used to verify the relationship between liquiritin and P53., Liquiritin alleviated pain sensitivity and improved gait impairment in OA mice. Additionally, we found that liquiritin could increase COL2 levels and decrease MMP13 levels both in vivo and in vitro. Importantly, liquiritin reduced chondrocyte apoptosis induced by OA, through decreased expression of caspase3 expression and increased expression of bcl2 expression. Molecular docking revealed a strong binding affinity between liquiritin and P53. Both in vivo and in vitro studies demonstrated that liquiritin suppressed the expression of P53 and PUMA in cartilage., This indicated that liquiritin may alleviate OA progression by inhibiting the P53/PUMA signaling pathway, suggesting that liquiritin is a potential strategy for the treatment of OA.
资助项目	Natural Science Foundation of Zhejiang Province[LY22H270005,LR23H270001];Chinese medicine scientific research projects[2022FSYYZY31];State Administration of Traditional Chinese Medicine of Zhejiang Province[2024ZL799,2024ZL052];Natural Science Foundation of China[82274550,82074457,82274280,82274549];Natural Science Foundation of Zhejiang Province[LY22H270005,LR23H270001];Natural Science Foundation of China[82274550,82274280,82104891,82274549];
出版者	PERGAMON-ELSEVIER SCIENCE LTD
ISSN	0024-3205
EISSN	1879-0631
卷号	343
DOI	10.1016/j.lfs.2024.122536
页数	11
WOS类目	Medicine, Research & Experimental ; Pharmacology & Pharmacy
WOS研究方向	Research & Experimental Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:001207053300001
收录类别	PUBMED ; SCOPUS ; SCIE
在线发表日期	2024-03
URL	查看原文
PubMed ID	3842317
SCOPUSEID	2-s2.0-85187326863
通讯作者地址	[Zhang, Chunchun]Institute of Orthopedics and Traumatology,The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine),Hangzhou,China
Scopus学科分类	Biochemistry, Genetics and Molecular Biology (all);Pharmacology, Toxicology and Pharmaceutics (all)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/210173
专题	附属第一医院其他_附属萧山医院（杭州市萧山区第一人民医院）
通讯作者	Zhang, Chunchun
作者单位	1.Institute of Orthopedics and Traumatology,The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine),Hangzhou,China; 2.College of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou,China; 3.The First College of Clinical Medicine,Zhejiang Chinese Medical University,Hangzhou,China; 4.The First People's Hospital of Xiaoshan District,Xiaoshan Affiliated Hospital of Wenzhou Medical University,Hangzhou,China; 5.Department of the Orthopedic Surgery,the Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China; 6.Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine,Wenzhou,China
推荐引用方式 GB/T 7714	Qiu, Min,Cheng, Liangyan,Xu, Jianbo,et al. Liquiritin reduces chondrocyte apoptosis through P53/PUMA signaling pathway to alleviate osteoarthritis[J]. Life sciences,2024,343.
APA	Qiu, Min., Cheng, Liangyan., Xu, Jianbo., Jin, Minwei., Yuan, Wenhua., ... & Wang, Pinger. (2024). Liquiritin reduces chondrocyte apoptosis through P53/PUMA signaling pathway to alleviate osteoarthritis. Life sciences, 343.
MLA	Qiu, Min,et al."Liquiritin reduces chondrocyte apoptosis through P53/PUMA signaling pathway to alleviate osteoarthritis".Life sciences 343(2024).