题名 | Staphylococcus aureus CC398: Host Adaptation and Emergence of Methicillin Resistance in Livestock |
作者 | Price, Lance B.1; Stegger, Marc2; Hasman, Henrik3; Aziz, Maliha1; Larsen, Jesper2; Andersen, Paal Skytt2; Pearson, Talima4; Waters, Andrew E.1; Foster, Jeffrey T.4; Schupp, James1; Gillece, John1; Driebe, Elizabeth1; Liu, Cindy M.1,4; Springer, Burkhard5; Zdovc, Irena6; Battisti, Antonio7; Franco, Alessia7; Zmudzki, Jacek8; Schwarz, Stefan9; Butaye, Patrick10,11; Jouy, Eric12; Pomba, Constanca13; Porrero, M. Concepción14; Ruimy, Raymond15; Smith, Tara C.16; Robinson, D. Ashley17; Weese, J. Scott18; Arriola, Carmen Sofia19; Yu, Fangyou20; Laurent, Frederic21; Keim, Paul1,4; Skov, Robert2; Aarestrup, Frank M.3
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发表日期 | 2012-01 |
发表期刊 | MBIO 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
其他关键词 | CASSETTE CHROMOSOME MEC ; COMPLEMENT INHIBITOR ; IMMUNE EVASION ; PIG FARMERS ; MRSA ; EVOLUTION ; POULTRY ; STRAINS ; HUMANS ; SPREAD |
摘要 | Since its discovery in the early 2000s, methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 398 (CC398) has become a rapidly emerging cause of human infections, most often associated with livestock exposure. We applied whole-genome sequence typing to characterize a diverse collection of CC398 isolates (n = 89), including MRSA and methicillin-susceptible S. aureus (MSSA) from animals and humans spanning 19 countries and four continents. We identified 4,238 single nucleotide polymorphisms (SNPs) among the 89 core genomes. Minimal homoplasy (consistency index = 0.9591) was detected among parsimony-informative SNPs, allowing for the generation of a highly accurate phylogenetic reconstruction of the CC398 clonal lineage. Phylogenetic analyses revealed that MSSA from humans formed the most ancestral clades. The most derived lineages were composed predominantly of livestock-associated MRSA possessing three different staphylococcal cassette chromosome mec element (SCCmec) types (IV, V, and VII-like) including nine subtypes. The human-associated isolates from the basal clades carried phages encoding human innate immune modulators that were largely missing among the livestock-associated isolates. Our results strongly suggest that livestock-associated MRSA CC398 originated in humans as MSSA. The lineage appears to have undergone a rapid radiation in conjunction with the jump from humans to livestock, where it subsequently acquired tetracycline and methicillin resistance. Further analyses are required to estimate the number of independent genetic events leading to the methicillin-resistant sublineages, but the diversity of SCCmec subtypes is suggestive of strong and diverse antimicrobial selection associated with food animal production. IMPORTANCE Modern food animal production is characterized by densely concentrated animals and routine antibiotic use, which may facilitate the emergence of novel antibiotic-resistant zoonotic pathogens. Our findings strongly support the idea that livestock-associated MRSA CC398 originated as MSSA in humans. The jump of CC398 from humans to livestock was accompanied by the loss of phage-carried human virulence genes, which likely attenuated its zoonotic potential, but it was also accompanied by the acquisition of tetracycline and methicillin resistance. Our findings exemplify a bidirectional zoonotic exchange and underscore the potential public health risks of widespread antibiotic use in food animal production. |
资助项目 | TGen Foundation; Statens Serum Institut; Center for Genomic Epidemiology [09-067103/DSF]; National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [GM080602]; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM080602] Funding Source: NIH RePORTER |
出版者 | AMER SOC MICROBIOLOGY |
出版地 | WASHINGTON |
ISSN | 2150-7511 |
卷号 | 3期号:1页码:1-6 |
DOI | 10.1128/mBio.00305-11 |
页数 | 6 |
WOS类目 | Microbiology |
WOS研究方向 | Microbiology |
WOS记录号 | WOS:000303331400022 |
收录类别 | SCIE ; SCOPUS |
URL | 查看原文 |
PubMed ID | 22354957 |
SCOPUSEID | 2-s2.0-84863242652 |
ESI高被引论文 | 2020-01 ; 2020-03 ; 2020-05 ; 2020-09 ; 2020-11 ; 2021-01 ; 2021-03 ; 2021-05 ; 2021-07 ; 2021-09 ; 2021-11 ; 2022-01 ; 2022-03 ; 2022-05 ; 2022-07 ; 2022-09 ; 2022-11 ; 2023-01 ; 2023-03 |
自科自定义期刊分类 | T3(B)类 |
通讯作者地址 | [Price L. B.]Translational Genomics Research Institute (TGen),Pathogen Genomics Division,United States |
Scopus学科分类 | Microbiology;Virology |
TOP期刊 | TOP期刊 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/20816 |
专题 | 附属第一医院 |
作者单位 | 1.Translational Genomics Research Institute (TGen),Pathogen Genomics Division,United States; 2.Department of Microbiological Surveillance and Research,Statens Serum Institut,Denmark; 3.National Food Institute,Technical University of Denmark,Denmark; 4.Center for Microbial Genetics and Genomics,Northern Arizona University,United States; 5.Österreichische AgenturfürGesundheit und Ernährungssicherheit GmbH,Bereich Humanmedizin,Nstitut für Medizinische Mikrobiologie und Hygiene,Austria; 6.Veterinary Faculty,Institute of Microbiology and Parasitology,University of Ljubljana,Slovenia; 7.IstitutoZooprofilattico Sperimentale delle Regioni Lazio eToscana,Italy; 8.Department of Swine Diseases,National Veterinary Research Institute,Poland; 9.Institute of Farm Animal Genetics,Friedrich-Loeffler-Institut,Germany; 10.Department of Pathology,Bacteriology,and Poultry Diseases,Faculty ofVeterinary Medicine,University of Ghent,Belgium; 11.Unit of General Bacteriology,Veterinary and Agrochemical Research Centre,Belgium; 12.Anses,Ploufragan Plouzané Laboratory,France; 13.Interdisciplinary Centre of Research in Animal Health,Faculty ofVeterinary Medicine,Portugal; 14.Centro de Vigilancia Sanitaria Veterinaria,Universidad Complutense de Madrid,Spain; 15.University Paris-Diderot and National Reference Center,Bacterial Resistance in the Commensal Flora,Hôpital Bichat-Claude Bernard,France; 16.Center for Emerging Infectious Diseases,University of Iowa,United States; 17.Department of Microbiology,University of Mississippi Medical Center,United States; 18.Ontario Veterinary College,University of Guelph,Canada; 19.Department of Preventive Veterinary Medicine,San Marcos Major National University,Peru; 20.Department of Laboratory Medicine,First Affiliated Hospital of Wenzhou Medical College,China; 21.Laboratory of Bacteriology,Hôpital de la Croix Rousse,National Reference Center for Staphylococci,France |
推荐引用方式 GB/T 7714 | Price, Lance B.,Stegger, Marc,Hasman, Henrik,et al. Staphylococcus aureus CC398: Host Adaptation and Emergence of Methicillin Resistance in Livestock[J]. MBIO,2012,3(1):1-6. |
APA | Price, Lance B.., Stegger, Marc., Hasman, Henrik., Aziz, Maliha., Larsen, Jesper., ... & Aarestrup, Frank M.. (2012). Staphylococcus aureus CC398: Host Adaptation and Emergence of Methicillin Resistance in Livestock. MBIO, 3(1), 1-6. |
MLA | Price, Lance B.,et al."Staphylococcus aureus CC398: Host Adaptation and Emergence of Methicillin Resistance in Livestock".MBIO 3.1(2012):1-6. |
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