Morphine compromises androgen biosynthesis by immature Leydig cells from pubertal rat testes in vitro

doi:10.1093/toxres/tfae001

科研成果详情

题名	Morphine compromises androgen biosynthesis by immature Leydig cells from pubertal rat testes in vitro
作者	Lv, Yao 1; Dong, Yaoyao2; Su, Ming2; Lin, Hang2; Zhu, Qiqi2; Li, Huitao2,3
发表日期	2024
发表期刊	Toxicology research 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	analgesic androgen production steroidogenic enzymes u opioid receptor
其他关键词	ACUTE REGULATORY PROTEIN ; LUTEINIZING-HORMONE ; RECEPTORS ; SERTOLI ; THERAPY ; PLASMA ; STAR ; PAIN
摘要	Morphine is an analgesic in the opiate family, isolated from many plants. It can inhibit androgen biosynthesis by Leydig cells. Whether morphine directly inhibits androgen biosynthesis and underlying mechanism remains unclear. To investigate the influence of morphine on androgen secretion by rat immature Leydig cells (ILCs) and possible mechanism. Rat ILCs were treated with 0.5-50 μM morphine for 3 h in vitro. Morphine at ≥0.5 μM significantly reduced total androgen secretion. Morphine at 50 μM also compromised luteinizing hormone (LH, 10 mg/kg), 8Br-cAMP (1 mM), and 22R-hydroxycholesterol (20 μM) stimulated total androgen, androstanediol, and testosterone secretion, without affecting pregnenolone, progesterone, androstenedione mediated androgen secretion and testosterone and dihydrotestosterone mediated androstanediol secretion. Further analysis revealed that morphine at ≥0.5 μM downregulated Star expression and at ≥5 μM downregulated Cyp11a1 expression. Morphine also significantly reduced STAR (≥0.5 μM) and reduced CYP11A1 (≥5 μM) levels. 0.5 μM naloxone significantly antagonized morphine-mediated action. In conclusion, morphine might cause side effects by suppressing androgen biosynthesis via u opioid receptor
资助项目	Wenzhou Medical University[KYYW202128];Zhejiang Provincial Natural Science Foundation[LQ22H040008];Wenzhou Municipal Science and Technology Bureau[Y20190703,Y20210253];
出版者	OXFORD UNIV PRESS
ISSN	2045-452X
EISSN	2045-4538
卷号	13 期号:1
DOI	10.1093/toxres/tfae001
页数	9
WOS类目	Toxicology
WOS研究方向	Toxicology
WOS记录号	WOS:001167119400012
收录类别	PUBMED ; SCOPUS ; SCIE
URL	查看原文
PubMed ID	38283823
SCOPUSEID	2-s2.0-85184493316
通讯作者地址	[Li, Huitao]Zhejiang Engineering Research Center for Innovation and Application of Intelligent Radiotherapy Technology,The Second Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China
Scopus学科分类	Toxicology;Health, Toxicology and Mutagenesis
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/207517
专题	附属第二医院第二临床医学院、附属第二医院、育英儿童医院附属第二医院_麻醉重点学科实验室
通讯作者	Li, Huitao
作者单位	1.Department of Pharmacy,Ningbo Medical Center Lihuili Hospital,Ningbo,Zhejiang,315100,China; 2.Department of Anesthesiology and Perioperative Medicine,The Second Affiliated Hospital,Yuying Children’s Hospital,Key Laboratory of Pediatric Anesthesiology,Ministry of Education,Key Laboratory of Anesthesiology of Zhejiang Province,Wenzhou Medical University,Wenzhou,Zhejiang,325027,China; 3.Zhejiang Engineering Research Center for Innovation,Application of Intelligent Radiotherapy Technology,The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou,Zhejiang,325000,China
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
推荐引用方式 GB/T 7714	Lv, Yao,Dong, Yaoyao,Su, Ming,et al. Morphine compromises androgen biosynthesis by immature Leydig cells from pubertal rat testes in vitro[J]. Toxicology research,2024,13(1).
APA	Lv, Yao, Dong, Yaoyao, Su, Ming, Lin, Hang, Zhu, Qiqi, & Li, Huitao. (2024). Morphine compromises androgen biosynthesis by immature Leydig cells from pubertal rat testes in vitro. Toxicology research, 13(1).
MLA	Lv, Yao,et al."Morphine compromises androgen biosynthesis by immature Leydig cells from pubertal rat testes in vitro".Toxicology research 13.1(2024).