FGF10 protects against LPS-induced epithelial barrier injury and inflammation by inhibiting SIRT1-ferroptosis pathway in acute lung injury in mice

doi:10.1016/j.intimp.2023.111426

科研成果详情

题名	FGF10 protects against LPS-induced epithelial barrier injury and inflammation by inhibiting SIRT1-ferroptosis pathway in acute lung injury in mice
作者	Lin, Lidan1; Yang, Li2; Wang, Nan1; Chen, Siyue3; Du, Xiaotong4; Chen, Ran1; Zhang, Hongyu4,5,6; Kong, Xiaoxia1,5
发表日期	2024-01-25
发表期刊	INTERNATIONAL IMMUNOPHARMACOLOGY 影响因子和分区
语种	英语
原始文献类型	Article ; Journal Article
关键词	Acute lung injury FGF10 Epithelial barrier Ferroptosis NRF2
其他关键词	KERATINOCYTE GROWTH FACTOR-2 ; NRF2 ; FERROPTOSIS ; SIRTUINS
摘要	Pulmonary alveolar epithelial cell injury is considered the main pathological and physiological change in acute lung injury. Ferroptosis in alveolar epithelial cells is one of crucial factors contributing to acute lung injury (ALI). Therefore, reducing ferroptosis and repair epithelial barrier is very necessary. More and more evidence suggested that FGF10 plays an important role in lung development and repair after injury. However, the relationship between FGF10 and ferroptosis remains unclear. This study aims to explore the regulatory role of FGF10 on ferroptosis in ALI. Differential gene expression analysis indicated that genes associated with ferroptosis showed that FGF10 can significantly alleviate LPS induced lung injury and epithelial barrier damage by decreasing levels of malonaldehyde(MDA), and lipid ROS. SIRT1 activator (Resveratrol) and inhibitor (EX527) are used in vivo showed that FGF10 protects ferroptosis of pulmonary epithelial cells through SIRT1 signal. Furthermore, knockdown of FGFR2 gene reduced the protective effect of FGF10 on acute lung injury in mice and SIRT1 activation. After the application of NRF2 inhibitor ML385 in vitro, the results showed that SIRT1 regulated the expression of ferroptosis related proteins NRF2, GPX4 and FTH1 are related to activation of NRF2. These data indicate that SIRT-ferroptosis was one of the critical mechanisms contributing to LPS-induced ALI. FGF10 is promising as a therapeutic candidate against ALI through inhibiting ferroptosis.
资助项目	Ningbo Natural Science Foundation of China, China [2022J043, 2021J032]; Foundation of Zhejiang Educational Committee, China [Y202044729]
出版者	ELSEVIER
ISSN	1567-5769
EISSN	1878-1705
卷号	127
DOI	10.1016/j.intimp.2023.111426
页数	11
WOS类目	Immunology ; Pharmacology & Pharmacy
WOS研究方向	Immunology ; Pharmacology & Pharmacy
WOS记录号	WOS:001146652500001
收录类别	SCIE ; PUBMED ; SCOPUS
在线发表日期	2023-12
URL	查看原文
PubMed ID	38147776
SCOPUSEID	2-s2.0-85180985556
通讯作者地址	[Kong, Xiaoxia]School of Basic Medical Sciences,Institute of Hypoxia Research,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China
Scopus学科分类	Immunology and Allergy;Immunology;Pharmacology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/207316
专题	基础医学院（机能实验教学中心）药学院（分析测试中心）附属第二医院附属第一医院第二临床医学院、附属第二医院、育英儿童医院第一临床医学院（信息与工程学院）、附属第一医院_内科学_呼吸与危重症医学科其他_附属诸暨医院（诸暨市人民医院）其他_温州医科大学慈溪生物医药研究院
通讯作者	Kong, Xiaoxia
作者单位	1.School of Basic Medical Sciences,Institute of Hypoxia Research,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China; 2.Department of Respiratory and Critical Care Medicine,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 3.Department of Children's Respiration disease,the Second Affiliated Hospital and Yuying Children's Hospital,Wenzhou Medical University,Zhejiang,Wenzhou,325000,China; 4.School of Pharmaceutical Sciences,Wenzhou Medical University,Zhejiang,315302,China; 5.Cixi Biomedical Research Institute,Wenzhou Medical University,Zhejiang,315302,China; 6.Department of Pharmacy,Zhuji People's Hospital,Wenzhou Medical University,Zhuji, Zhejiang,Shaoxing,311800,China
第一作者单位	基础医学院（机能实验教学中心）
通讯作者单位	基础医学院（机能实验教学中心）
第一作者的第一单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Lin, Lidan,Yang, Li,Wang, Nan,et al. FGF10 protects against LPS-induced epithelial barrier injury and inflammation by inhibiting SIRT1-ferroptosis pathway in acute lung injury in mice[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2024,127.
APA	Lin, Lidan., Yang, Li., Wang, Nan., Chen, Siyue., Du, Xiaotong., ... & Kong, Xiaoxia. (2024). FGF10 protects against LPS-induced epithelial barrier injury and inflammation by inhibiting SIRT1-ferroptosis pathway in acute lung injury in mice. INTERNATIONAL IMMUNOPHARMACOLOGY, 127.
MLA	Lin, Lidan,et al."FGF10 protects against LPS-induced epithelial barrier injury and inflammation by inhibiting SIRT1-ferroptosis pathway in acute lung injury in mice".INTERNATIONAL IMMUNOPHARMACOLOGY 127(2024).