题名 | A synthetic microbial consortium protects against obesity by regulating vitamin B6 metabolism |
作者 | |
发表期刊 | GUT MICROBES 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
关键词 | Synthetic microbial consortia obesity gut microbiota metabolomics microbial interaction engineered probiotics |
摘要 | Constructing synthetic microbial consortia is a challenging task but holds enormous potential for various applications. Our previous droplet-based microfluidic approach allowed for the isolation of bacteria that could utilize metabolites from an engineered bacterium BsS-RS06551 with anti-obesity potential, facilitating the construction of synthetic microbial consortia. Here, we identified a strain of Bifidobacterium pseudocatenulatum JJ3 that interacted with BsS-RS06551, and in vitro coculture showed that BsS-RS06551 was likely to interact with JJ3 through five dipeptides. Pathway analysis revealed that the vitamin B6 metabolism pathway was enriched in the coculture of BsS-RS06551 and JJ3 compared with the individual culture of BsS-RS06551. Additionally, we confirmed that the administration of JJ3 significantly alleviated obesity and related disorders in mice fed a high-fat diet. Notably, continuous ingestion of the synthetic microbial consortium comprising BsS-RS06551 and JJ3 not only exhibited a more pronounced impact on alleviating obesity compared to the individual administration of BsS-RS06551 or JJ3 but also enriched the population of Bifidobacterium longum and perturbed the vitamin B6 metabolism pathway in the gut. Synthetic microbial consortia represent a promising frontier for synthetic biology, and our strategy provides guidance for constructing and applying such consortia. |
资助项目 | National Key Research and Development Project [2019YFA0905600]; Science and Technology Program of Tianjin, China [22YFZCSN00090]; China-CEEC Joint Education Project [2022196] |
出版者 | TAYLOR & FRANCIS INC |
ISSN | 1949-0976 |
EISSN | 1949-0984 |
卷号 | 16期号:1 |
DOI | 10.1080/19490976.2024.2304901 |
页数 | 21 |
WOS类目 | Gastroenterology & Hepatology ; Microbiology |
WOS研究方向 | Gastroenterology & Hepatology ; Microbiology |
WOS记录号 | WOS:001148673500001 |
收录类别 | SCIE ; PUBMED ; SCOPUS |
发表日期 | 2024-12-31 |
URL | 查看原文 |
Pubmed记录号 | 38269591 |
Scopus记录号 | 2-s2.0-85182989111 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/206932 |
专题 | 温州医科大学 |
通讯作者 | Huang, He; Kang, Guangbo |
作者单位 | 1.Tianjin Univ, Frontiers Sci Ctr Synthet Biol, Sch Chem Engn & Technol, Key Lab Syst Bioengn, Tianjin, Peoples R China; 2.Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, State Key Lab Bioact Substance & Funct Nat Med, Beijing 100050, Peoples R China; 3.Wenzhou Med Univ, Oujiang Lab, Wenzhou, Zhejiang, Peoples R China; 4.Tianji Univ, Frontiers Sci Ctr Synthet Biol, Sch Chem Engn & Technol, Key Lab Syst Bioengn, Tianjin 300350, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Xiuzhao,Gao, Mengxue,Wang, Lina,et al. A synthetic microbial consortium protects against obesity by regulating vitamin B6 metabolism[J]. GUT MICROBES,2024,16(1). |
APA | Chen, Xiuzhao., Gao, Mengxue., Wang, Lina., Qiang, Guifen., Wu, Yili., ... & Kang, Guangbo. (2024). A synthetic microbial consortium protects against obesity by regulating vitamin B6 metabolism. GUT MICROBES, 16(1). |
MLA | Chen, Xiuzhao,et al."A synthetic microbial consortium protects against obesity by regulating vitamin B6 metabolism".GUT MICROBES 16.1(2024). |
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