A synthetic microbial consortium protects against obesity by regulating vitamin B6 metabolism

doi:10.1080/19490976.2024.2304901

科研成果详情

题名	A synthetic microbial consortium protects against obesity by regulating vitamin B6 metabolism
作者	Chen, Xiuzhao 1; Gao, Mengxue 1; Wang, Lina 1; Qiang, Guifen 2; Wu, Yili3; Huang, He 1; Kang, Guangbo 1
发表日期	2024-12-31
发表期刊	GUT MICROBES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Synthetic microbial consortia obesity gut microbiota metabolomics microbial interaction engineered probiotics
摘要	Constructing synthetic microbial consortia is a challenging task but holds enormous potential for various applications. Our previous droplet-based microfluidic approach allowed for the isolation of bacteria that could utilize metabolites from an engineered bacterium BsS-RS06551 with anti-obesity potential, facilitating the construction of synthetic microbial consortia. Here, we identified a strain of Bifidobacterium pseudocatenulatum JJ3 that interacted with BsS-RS06551, and in vitro coculture showed that BsS-RS06551 was likely to interact with JJ3 through five dipeptides. Pathway analysis revealed that the vitamin B6 metabolism pathway was enriched in the coculture of BsS-RS06551 and JJ3 compared with the individual culture of BsS-RS06551. Additionally, we confirmed that the administration of JJ3 significantly alleviated obesity and related disorders in mice fed a high-fat diet. Notably, continuous ingestion of the synthetic microbial consortium comprising BsS-RS06551 and JJ3 not only exhibited a more pronounced impact on alleviating obesity compared to the individual administration of BsS-RS06551 or JJ3 but also enriched the population of Bifidobacterium longum and perturbed the vitamin B6 metabolism pathway in the gut. Synthetic microbial consortia represent a promising frontier for synthetic biology, and our strategy provides guidance for constructing and applying such consortia.
资助项目	National Key Research and Development Project [2019YFA0905600]; Science and Technology Program of Tianjin, China [22YFZCSN00090]; China-CEEC Joint Education Project [2022196]
出版者	TAYLOR & FRANCIS INC
ISSN	1949-0976
EISSN	1949-0984
卷号	16 期号:1
DOI	10.1080/19490976.2024.2304901
页数	21
WOS类目	Gastroenterology & Hepatology ; Microbiology
WOS研究方向	Gastroenterology & Hepatology ; Microbiology
WOS记录号	WOS:001148673500001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	38269591
SCOPUSEID	2-s2.0-85182989111
通讯作者地址	[Huang, He]Frontiers Science Center for Synthetic Biology,Key Laboratory of Systems Bioengineering (Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin,300350,China
Scopus学科分类	Microbiology;Microbiology (medical);Gastroenterology;Infectious Diseases
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/206932
专题	其他_瓯江实验室
通讯作者	Huang, He
作者单位	1.Frontiers Science Center for Synthetic Biology,Key Laboratory of Systems Bioengineering (Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin,China; 2.State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences,Peking Union Medical College and Beijing Key Laboratory of Drug Target and Screening Research,Beijing,China; 3.Oujiang Laboratory,Wenzhou Medical University,Zhejiang,Wenzhou,China
推荐引用方式 GB/T 7714	Chen, Xiuzhao,Gao, Mengxue,Wang, Lina,et al. A synthetic microbial consortium protects against obesity by regulating vitamin B6 metabolism[J]. GUT MICROBES,2024,16(1).
APA	Chen, Xiuzhao., Gao, Mengxue., Wang, Lina., Qiang, Guifen., Wu, Yili., ... & Kang, Guangbo. (2024). A synthetic microbial consortium protects against obesity by regulating vitamin B6 metabolism. GUT MICROBES, 16(1).
MLA	Chen, Xiuzhao,et al."A synthetic microbial consortium protects against obesity by regulating vitamin B6 metabolism".GUT MICROBES 16.1(2024).