Palmatine ameliorated lipopolysaccharide-induced sepsis-associated encephalopathy mice by regulating the microbiota-gut-brain axis

doi:10.1016/j.phymed.2023.155307

科研成果详情

题名	Palmatine ameliorated lipopolysaccharide-induced sepsis-associated encephalopathy mice by regulating the microbiota-gut-brain axis
作者	Xu, Yubin 1; Shen, Bixin2; Pan, Xusheng 1; Liu, Chang 3; Wang, Yingyue 4; Chen, Xiaowei 4; Wang, Ting 1,5; Chen, Guirong 3,6; Chen, Jing7
发表日期	2024-02
发表期刊	PHYTOMEDICINE 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access ; Journal Article
关键词	Palmatine Sepsis-associated encephalopathy Gut microbiota Notch1/NF-kappa B pathway Inflammatory storm Notch1/NF-κB pathway
其他关键词	AFFINITY
摘要	Background: Sepsis-associated encephalopathy (SAE), a common neurological complication from sepsis, is widespread among patients in intensive care unit and is linked to substantial morbidity and mortality rates, thus posing a substantial menace to human health. Due to the intricate nature of SAE's pathogenesis, there remains a dearth of efficacious therapeutic protocols, encompassing pharmaceutical agents and treatment modalities, up until the present time. Palmatine exhibits distinctive benefits in the regulation of inflammation for the improvement of sepsis. Nevertheless, the precise functions of palmatine in treating SAE and its underlying mechanism have yet to be elucidated. Purpose: This study aimed to evaluate efficiency of palmatine in SAE mice and its underlying mechanisms. Study design and methods: Behavioral experiments, percent survival rate analysis, histological analysis, immunofluorescence staining, ELISA analysis, were performed to evaluate the efficiency of palmatine in SAE mice. Quantibody (R) mouse inflammation array glass chip was performed to observe the effects of palmatine on inflammation storm in SAE mice. Real-time quantitative and western blotting analyzes were employed to examine the expression of relevant targets in the Notch1/nuclear factor-kappa B (NF-kappa B) pathway. Finally, brain tissues metabolomics-based analyzes were performed to detect the differentially expressed metabolites and metabolic pathways. The fecal samples were subjected to microbial 16S rRNA analysis and untargeted metabolomics analysis in order to identify the specific flora and metabolites associated with SAE, thereby further investigating the mechanism of palmatine in SAE mice. Results: Our results showed that palmatine significantly improved nerve function, reduced cell apoptosis in brain tissue, and decreased inflammatory cytokine levels in SAE induced-LPS mice. Meanwhile, our results demonstrate the potential of palmatine in modulating key components of the Notch1/NF-kappa B pathway, enhancing the expression of tight junction proteins, improving intestinal permeability, promoting the growth of beneficial bacteria (such as Lachnospiraceae_NK4A136_group), inhibiting the proliferation of harmful bacteria (such as Escherichia-Shigella), and mitigating metabolic disorders. Ultimately, these observed effects contribute to the therapeutic efficacy of palmatine in treating SAE. Conclusion: The findings of our study have provided confirmation regarding the efficacy of palmatine in the treatment of SAE, thereby establishing a solid foundation for further exploration into SAE therapy and the advancement and investigation of palmatine.
资助项目	Open fund of Zhejiang Province Health Innovation Talents; Natural Science Foundation of China [82374008]; Zhejiang Provincial Medical and Health Science and Technology Plan Project [2024KY1823]; Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications [zyzx2206]; Taizhou Science and Technology plan project [21ywa33, 21ywa01]; Clinical Research Fund project of Clinical Rational Drug Use Committee of Zhejiang Medical Doctor Association [YS2022-2-008]
出版者	ELSEVIER GMBH
ISSN	0944-7113
EISSN	1618-095X
卷号	124
DOI	10.1016/j.phymed.2023.155307
页数	12
WOS类目	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS记录号	WOS:001155975900001
收录类别	SCIE ; PUBMED ; SCOPUS
在线发表日期	2024-01
URL	查看原文
PubMed ID	38181529
SCOPUSEID	2-s2.0-85181816324
通讯作者地址	[Wang, Ting]Department of Pharmacy,Taizhou Central Hospital (Taizhou University Hospital),Zhejiang,Taizhou,318000,China
Scopus学科分类	Molecular Medicine;Pharmacology;Pharmaceutical Science;Drug Discovery;Complementary and Alternative Medicine
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/206925
专题	药学院（分析测试中心）其他_附属台州医院（浙江省台州医院）
通讯作者	Wang, Ting
作者单位	1.Department of Pharmacy,Taizhou Central Hospital (Taizhou University Hospital),Zhejiang,Taizhou,318000,China; 2.Department of Pharmaceutics,School of Pharmaceutical Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,325000,China; 3.College of pharmacy,Liaoning University of Traditional Chinese Medicine,Liaoning,Shenyang,110847,China; 4.College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin,300000,China; 5.School of Medicine,Taizhou University,Zhejiang,Taizhou,318000,China; 6.Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications,Liaoning University of Traditional Chinese Medicine,Liaoning,Shenyang,110847,China; 7.Department of Pharmacy,Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University,Zhejiang,Lin Hai,317000,China
推荐引用方式 GB/T 7714	Xu, Yubin,Shen, Bixin,Pan, Xusheng,et al. Palmatine ameliorated lipopolysaccharide-induced sepsis-associated encephalopathy mice by regulating the microbiota-gut-brain axis[J]. PHYTOMEDICINE,2024,124.
APA	Xu, Yubin., Shen, Bixin., Pan, Xusheng., Liu, Chang., Wang, Yingyue., ... & Chen, Jing. (2024). Palmatine ameliorated lipopolysaccharide-induced sepsis-associated encephalopathy mice by regulating the microbiota-gut-brain axis. PHYTOMEDICINE, 124.
MLA	Xu, Yubin,et al."Palmatine ameliorated lipopolysaccharide-induced sepsis-associated encephalopathy mice by regulating the microbiota-gut-brain axis".PHYTOMEDICINE 124(2024).