B19, a Novel Monocarbonyl Analogue of Curcumin, Induces Human Ovarian Cancer Cell Apoptosis via Activation of Endoplasmic Reticulum Stress and the Autophagy Signaling Pathway

doi:10.7150/ijbs.5711

科研成果详情

题名	B19, a Novel Monocarbonyl Analogue of Curcumin, Induces Human Ovarian Cancer Cell Apoptosis via Activation of Endoplasmic Reticulum Stress and the Autophagy Signaling Pathway
作者	Qu, Wanglei1; Xiao, Jian2; Zhang, Hongyu2; Chen, Qiong 1; Wang, Zhouguang 2; Shi, Hongxue2; Gong, Liang3; Chen, Jianqiang 3; Liu, Yanlong2; Cao, Risheng 4; Lv, Jieqiang1
发表日期	2013-12-31
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Curcumin analogues B19 ovarian cancer apoptosis ER stress autophagy
其他关键词	ER STRESS ; DEPENDENT PATHWAYS ; TUMOR-CELLS ; DEATH ; CISPLATIN ; DEGRADATION ; INHIBITION ; PACLITAXEL ; PROTEINS ; MELANOMA
摘要	Background: The unfolded protein response, autophagy and endoplasmic reticulum (ER) stress-induced apoptosis regulate tumor cell fate and have become novel signaling targets for the development of cancer therapeutic drugs. Curcumin has been used to treat several different cancers, including ovarian cancer, in clinical trials and research; however, the role of ER stress and autophagy in the therapeutic effects of curcumin and new curcumin analogues remains unclear. Methods: Cell viability was determined using the MTT assay. Apoptosis was detected using flow cytometry with PI/Annexin V-FITC staining. The expression levels of ER stress-and autophagy-related proteins were analyzed by western blotting. The activation of autophagy was detected using immunofluorescence staining. Results: We demonstrated that B19 induced HO8910 cell apoptosis in a dose-responsive manner. We also determined and that this effect was associated with corresponding increases in a series of key components in the UPR and ER stress-mediated apoptosis pathways, followed by caspase 3 cleavage and activation. We also observed that B19 treatment induced autophagy in HO8910 cells. The inhibition of autophagy using 3-methyladenine (3-MA) increased levels of intracellular misfolded proteins, which enhanced ovarian cancer apoptosis. Conclusions: Our data indicate that ER stress and autophagy may play a role in the apoptosis that is induced by the curcumin analogue B19 in an epithelial ovarian cancer cell line and that autophagy inhibition can increase curcumin analogue-induced apoptosis by inducing severe ER stress.
资助项目	National Natural Science Funding of ChinaNational Natural Science Foundation of China (NSFC) [30901819]; Natural Science Funding of Zhejiang province [LY12H13002]; Zhejiang Provincial Project of Key Group [2010R50042]; Key project of the Zhejiang traditional Chinese medicine administration [2012ZZ012]; Science Foundation of Ninbo city [2012A610206]
出版者	IVYSPRING INT PUBL
出版地	LAKE HAVEN
ISSN	1449-2288
卷号	9 期号:8 页码:766-777
DOI	10.7150/ijbs.5711
页数	12
WOS类目	Biochemistry & Molecular Biology ; Biology
WOS研究方向	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS记录号	WOS:000324419200002
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	23983610
PMC记录号	PMC3753441
SCOPUSEID	2-s2.0-84884253766
通讯作者地址	[Lv, Jieqiang]Department of Gynecology and Obstetrics,The Second Affiliated Hospital,Wenzhou Medical University,China
Scopus学科分类	Ecology, Evolution, Behavior and Systematics;Applied Microbiology and Biotechnology;Molecular Biology;Developmental Biology;Cell Biology
引用统计	被引频次：48[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/2002
专题	药学院（分析测试中心）附属第二医院第二临床医学院、附属第二医院、育英儿童医院研究生工作部（研究生院）其他_附属慈溪医院（慈溪市人民医院）
通讯作者	Lv, Jieqiang
作者单位	1.Department of Gynecology and Obstetrics,The Second Affiliated Hospital,Wenzhou Medical University,China; 2.School of Pharmacy,Key Laboratory of Biotechnology and Pharmaceutical Engineering,Wenzhou Medical University,China; 3.Department of otolaryngology,Cixi Hospital,Wenzhou Medical University,China; 4.Department of Gastroenterology,The First Affiliated Hospital,Nanjing Medical University,China
第一作者单位	附属第二医院
通讯作者单位	附属第二医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Qu, Wanglei,Xiao, Jian,Zhang, Hongyu,et al. B19, a Novel Monocarbonyl Analogue of Curcumin, Induces Human Ovarian Cancer Cell Apoptosis via Activation of Endoplasmic Reticulum Stress and the Autophagy Signaling Pathway[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2013,9(8):766-777.
APA	Qu, Wanglei., Xiao, Jian., Zhang, Hongyu., Chen, Qiong., Wang, Zhouguang., ... & Lv, Jieqiang. (2013). B19, a Novel Monocarbonyl Analogue of Curcumin, Induces Human Ovarian Cancer Cell Apoptosis via Activation of Endoplasmic Reticulum Stress and the Autophagy Signaling Pathway. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 9(8), 766-777.
MLA	Qu, Wanglei,et al."B19, a Novel Monocarbonyl Analogue of Curcumin, Induces Human Ovarian Cancer Cell Apoptosis via Activation of Endoplasmic Reticulum Stress and the Autophagy Signaling Pathway".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 9.8(2013):766-777.