His-87 ligand in mitoNEET is crucial for the transfer of iron sulfur clusters from mitochondria to cytosolic aconitase

doi:10.1016/j.bbrc.2016.01.040

科研成果详情

题名	His-87 ligand in mitoNEET is crucial for the transfer of iron sulfur clusters from mitochondria to cytosolic aconitase
作者	Tan, Guoqiang1; Liu, Danhui1; Pan, Feiyan 1; Zhao, Jin 1; Li, Tang 1; Ma, Yin 1; Shen, Bo2; Lyu, Jianxin 1
发表日期	2016-01-29
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	MitoNEET His-87 ligand mutations Aconitase Iron-sulfur cluster
其他关键词	DRUG TARGET MITONEET ; MEMBRANE PROTEIN ; 2FE-2S CLUSTER ; BINDING ; MUTATION ; REVEALS
摘要	MitoNEET is the first identified iron sulfur protein that located in the mitochondrial outer membrane. We showed that knockdown of mitoNEET did not affect the iron sulfur protein expression in mitochondria and cytoplasm, but significantly reduced the cytosolic aconitase activity. The reduction of aconitase activity was rescued by transfection of wild type mitoNEET, but not by mitoNEET mutants H87C and H87S. Our results confirm the observation that mitoNEET is important in transferring the iron sulfur clusters to the cytosolic aconitase in living cells and the His-87 ligand in mitoNEET plays important role in this process. (C) 2016 Elsevier Inc. All rights reserved.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31200587, 81500440, 81301744]; Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LY12C05003]; Key Science and Technology Innovation Team of Zhejiang Province [2010R50048-14]
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
出版地	SAN DIEGO
ISSN	0006-291X
EISSN	1090-2104
卷号	470 期号:1 页码:226-232
DOI	10.1016/j.bbrc.2016.01.040
页数	7
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000369678100035
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	26778000
SCOPUSEID	2-s2.0-84956574430
通讯作者地址	[Tan, Guoqiang]Key Laboratory of Laboratory Medicine,Ministry of Education,Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms,Institute of Functional Nucleic Acids and Personalized Cancer Theranostics,College of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Biophysics;Biochemistry;Molecular Biology;Cell Biology
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/19378
专题	检验医学院（生命科学学院、生物学实验教学中心）检验医学院（生命科学学院、生物学实验教学中心）_模式生物技术与应用重点实验室其他_附属台州医院（浙江省台州医院）
通讯作者	Tan, Guoqiang
作者单位	1.Key Laboratory of Laboratory Medicine,Ministry of Education,Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms,Institute of Functional Nucleic Acids and Personalized Cancer Theranostics,College of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,325035,China; 2.Taizhou Hospital,Wenzhou Medical University,Taizhou,317000,China
第一作者单位	模式生物技术与应用重点实验室; 检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	模式生物技术与应用重点实验室; 检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	模式生物技术与应用重点实验室
推荐引用方式 GB/T 7714	Tan, Guoqiang,Liu, Danhui,Pan, Feiyan,et al. His-87 ligand in mitoNEET is crucial for the transfer of iron sulfur clusters from mitochondria to cytosolic aconitase[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,470(1):226-232.
APA	Tan, Guoqiang., Liu, Danhui., Pan, Feiyan., Zhao, Jin., Li, Tang., ... & Lyu, Jianxin. (2016). His-87 ligand in mitoNEET is crucial for the transfer of iron sulfur clusters from mitochondria to cytosolic aconitase. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 470(1), 226-232.
MLA	Tan, Guoqiang,et al."His-87 ligand in mitoNEET is crucial for the transfer of iron sulfur clusters from mitochondria to cytosolic aconitase".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 470.1(2016):226-232.