Evaluation of Tumor-Derived Exosomal miRNA as Potential Diagnostic Biomarkers for Early-Stage Non-Small Cell Lung Cancer Using Next-Generation Sequencing

doi:10.1158/1078-0432.CCR-17-0577

科研成果详情

题名	Evaluation of Tumor-Derived Exosomal miRNA as Potential Diagnostic Biomarkers for Early-Stage Non-Small Cell Lung Cancer Using Next-Generation Sequencing
作者	Jin, Xiance1; Chen, Yanfan2; Chen, Hanbin 1; Fei, Shaoran 1; Chen, Didi1; Cai, Xiaona1; Liu, Linger 1; Lin, Baochai1; Su, Huafang1; Zhao, Lihao1; Su, Meng 1; Pan, Huanle 1; Shen, Lanxiao1; Xie, Deyao3; Xie, Congyingd 1
发表日期	2017-09-01
发表期刊	CLINICAL CANCER RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	MICRORNA SIGNATURES ; CIRCULATING MIRNA ; PREDICTS SURVIVAL ; EXPRESSION ; CARCINOMA ; MARKERS ; IDENTIFICATION ; CHEMOTHERAPY ; STATISTICS ; PROGNOSIS
摘要	Purpose: To identify tumor-derived exosomal biomarkers that are able to discriminate between adenocarcinoma and squamous cell carcinoma (SCC) as a noninvasive method in the early diagnosis of non-small cell lung cancer (NSCLC). Experimental Design: Tumor-derived exosomes from the plasma of early-stage NSCLC patients were isolated. Exosomal miRNA profiling of 46 stage I NSCLC patients and 42 healthy individuals was performed using miRNA-seq to identify and validate adenocarcinoma- and SCC-specific miRNAs. The diagnostic accuracy of select miRNAs was tested further with an additional 60 individuals. Results: There were 11 and 6 miRNAs expressed at remarkably higher levels, 13 and 8 miRNAs expressed at lower levels in adenocarcinoma and SCC patients, respectively, compared with healthy volunteers. Distinct adenocarcinoma- and SCC-specific exosomal miRNAs were validated. The reliability of miRNA-seq data was verified with several demonstrated diagnostic potential miRNAs for NSCLC and other carcinomas, as reported in previous studies, such as let-7, miR-21, miR-24, and miR-486. The results indicated that miR-181-5p, miR-30a-3p, miR-30e-3p, and miR-361-5p were adenocarcinoma-specific, and miR-10b-5p, miR-15b-5p, and miR-320b were SCC-specific. The diagnostic accuracy of three combination miRNA panels was evaluated using an AUC value of 0.899, 0.936, and 0.911 for detecting NSCLC, adenocarcinoma, and SCC, respectively. Conclusions: Tumor-derived exosomal miRNAs, adenocarcinoma-specific miR-181-5p, miR-30a-3p, miR-30e-3p and miR-361-5p, and SCC-specific miR-10b-5p, miR-15b-5p, and miR-320b were observed by next-generation sequencing, and their diagnostic accuracy were verified. These miRNAs may be promising and effective candidates in the development of highly sensitive, noninvasive biomarkers for early NSCLC diagnosis. (C) 2017 AACR.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [11675122]; Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LY16H160046, LY17H160051]
出版者	AMER ASSOC CANCER RESEARCH
出版地	PHILADELPHIA
ISSN	1078-0432
EISSN	1557-3265
卷号	23 期号:17 页码:5311-5319
DOI	10.1158/1078-0432.CCR-17-0577
页数	9
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000409037300036
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28606918
SCOPUSEID	2-s2.0-85029455426
ESI高被引论文	2020-05 ; 2020-09 ; 2020-11 ; 2021-01 ; 2021-03 ; 2021-05 ; 2021-07 ; 2021-11 ; 2022-01 ; 2022-03 ; 2022-07 ; 2022-09 ; 2022-11 ; 2023-01 ; 2023-03 ; 2023-05 ; 2023-07 ; 2023-09 ; 2023-11 ; 2024-01 ; 2024-03 ; 2024-05 ; 2024-07 ; 2024-09 ; 2024-11
自科自定义期刊分类	T3(A)类
通讯作者地址	[Xie, Congyingd]Department of Radiotherapy and Chemotherapy,First Affiliated Hospital of Wenzhou Medical University,No. 2 Fuxue Lane,Lucheng District, Wenzhou,325000,China
Scopus学科分类	Oncology;Cancer Research
TOP期刊	TOP期刊
引用统计	被引频次：302[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/19349
专题	附属第一医院
通讯作者	Xie, Congyingd
作者单位	1.Department of Radiotherapy and Chemotherapy,First Affiliated Hospital of Wenzhou Medical University,No. 2 Fuxue Lane,Lucheng District, Wenzhou,325000,China; 2.Department of Respiratory,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 3.Department of Thoracic Surgery,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Jin, Xiance,Chen, Yanfan,Chen, Hanbin,et al. Evaluation of Tumor-Derived Exosomal miRNA as Potential Diagnostic Biomarkers for Early-Stage Non-Small Cell Lung Cancer Using Next-Generation Sequencing[J]. CLINICAL CANCER RESEARCH,2017,23(17):5311-5319.
APA	Jin, Xiance., Chen, Yanfan., Chen, Hanbin., Fei, Shaoran., Chen, Didi., ... & Xie, Congyingd. (2017). Evaluation of Tumor-Derived Exosomal miRNA as Potential Diagnostic Biomarkers for Early-Stage Non-Small Cell Lung Cancer Using Next-Generation Sequencing. CLINICAL CANCER RESEARCH, 23(17), 5311-5319.
MLA	Jin, Xiance,et al."Evaluation of Tumor-Derived Exosomal miRNA as Potential Diagnostic Biomarkers for Early-Stage Non-Small Cell Lung Cancer Using Next-Generation Sequencing".CLINICAL CANCER RESEARCH 23.17(2017):5311-5319.