Inhibitory effects of 2-methoxyestradiol on cell growth and invasion in human bladder cancer T-24 cells

doi:10.1691/ph.2017.6839

科研成果详情

题名	Inhibitory effects of 2-methoxyestradiol on cell growth and invasion in human bladder cancer T-24 cells
作者	Huang X.X.1; Wang R.X.2; Lin Q.1; Chen W.1; Pan Y.1; Wang S.Q.1; Weng Z.L.1; Huang W.P.1
发表日期	2017-02
发表期刊	PHARMAZIE 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	HYPOXIA-INDUCIBLE FACTOR-1-ALPHA ; TUMOR-GROWTH ; HEPATOCELLULAR-CARCINOMA ; FACTORS HIF-1-ALPHA ; FACTOR 1-ALPHA ; NECK-CANCER ; MOUSE MODEL ; FACTOR-I ; ANGIOGENESIS ; EXPRESSION
摘要	Recently, 2-methoxyestradiol (2-ME) has been considered to be a potential anticancer agent but has not been investigated in bladder cancer. This study was conducted to clarify the role of 2-ME in bladder cancer cells. The bladder cancer cell line T-24 was treated with 2 pm 2-ME for 2 d. The T-24 cell viability, colony formation, invasion and apoptosis were observed in 2-ME-treated and control cells. The expression of hypoxia-inducible factor 1 alpha (HIF-1 alpha) was detected using reverse transcription-polymerase chain reaction (RT-PCR). Then western blotting assay was applied to assess expressions of HIF-1a and apoptosis factors caspase-3 and Bcl-x proteins. The mRNA and protein expressions of HIF-1a in 2-ME-treated T-24 cells were remarkably lower than that of the control cells (P < 0.05). Treatment of 2-ME could significantly inhibit T-24 the cell viability, colony formation, invasion, and promote apoptosis (all P < 0.05). In addition, the protein expression of Caspase-3 was higher and that of Bcl-x protein was lower after administration of 2-ME compared to control (both P < 0.05). Collectively, we characterized the efficacy of 2-ME on bladder cancer T-24 cells as being mediated by inhibition of cell viability, colony fomation, invasion and promoting cell apoptosis, which may be achieved by suppressing HIF-l alpha levels. This study suggests 2-ME as a potential drug for bladder cancer therapy.
出版者	GOVI-VERLAG PHARMAZEUTISCHER VERLAG GMBH
出版地	ESCHBORN
ISSN	0031-7144
卷号	72 期号:2 页码:87-90
DOI	10.1691/ph.2017.6839
页数	4
WOS类目	Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy ; Chemistry
WOS记录号	WOS:000397353600004
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	29441858
SCOPUSEID	2-s2.0-85013231687
通讯作者地址	[Huang W.P.]Department of Urology,Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Pharmaceutical Science
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/19024
专题	附属第一医院附属第二医院第二临床医学院、附属第二医院、育英儿童医院第一临床医学院（信息与工程学院）、附属第一医院_麻醉科
通讯作者	Huang W.P.
作者单位	1.Department of Urology,Wenzhou Medical University,Wenzhou,China; 2.Department of Anesthesiology,Wenzhou Medical University,Wenzhou,China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Huang X.X.,Wang R.X.,Lin Q.,et al. Inhibitory effects of 2-methoxyestradiol on cell growth and invasion in human bladder cancer T-24 cells[J]. PHARMAZIE,2017,72(2):87-90.
APA	Huang X.X.., Wang R.X.., Lin Q.., Chen W.., Pan Y.., ... & Huang W.P.. (2017). Inhibitory effects of 2-methoxyestradiol on cell growth and invasion in human bladder cancer T-24 cells. PHARMAZIE, 72(2), 87-90.
MLA	Huang X.X.,et al."Inhibitory effects of 2-methoxyestradiol on cell growth and invasion in human bladder cancer T-24 cells".PHARMAZIE 72.2(2017):87-90.