题名 | Inhibitory effects of 2-methoxyestradiol on cell growth and invasion in human bladder cancer T-24 cells |
作者 | |
发表日期 | 2017-02 |
发表期刊 | PHARMAZIE 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
其他关键词 | HYPOXIA-INDUCIBLE FACTOR-1-ALPHA ; TUMOR-GROWTH ; HEPATOCELLULAR-CARCINOMA ; FACTORS HIF-1-ALPHA ; FACTOR 1-ALPHA ; NECK-CANCER ; MOUSE MODEL ; FACTOR-I ; ANGIOGENESIS ; EXPRESSION |
摘要 | Recently, 2-methoxyestradiol (2-ME) has been considered to be a potential anticancer agent but has not been investigated in bladder cancer. This study was conducted to clarify the role of 2-ME in bladder cancer cells. The bladder cancer cell line T-24 was treated with 2 pm 2-ME for 2 d. The T-24 cell viability, colony formation, invasion and apoptosis were observed in 2-ME-treated and control cells. The expression of hypoxia-inducible factor 1 alpha (HIF-1 alpha) was detected using reverse transcription-polymerase chain reaction (RT-PCR). Then western blotting assay was applied to assess expressions of HIF-1a and apoptosis factors caspase-3 and Bcl-x proteins. The mRNA and protein expressions of HIF-1a in 2-ME-treated T-24 cells were remarkably lower than that of the control cells (P < 0.05). Treatment of 2-ME could significantly inhibit T-24 the cell viability, colony formation, invasion, and promote apoptosis (all P < 0.05). In addition, the protein expression of Caspase-3 was higher and that of Bcl-x protein was lower after administration of 2-ME compared to control (both P < 0.05). Collectively, we characterized the efficacy of 2-ME on bladder cancer T-24 cells as being mediated by inhibition of cell viability, colony fomation, invasion and promoting cell apoptosis, which may be achieved by suppressing HIF-l alpha levels. This study suggests 2-ME as a potential drug for bladder cancer therapy. |
出版者 | GOVI-VERLAG PHARMAZEUTISCHER VERLAG GMBH |
出版地 | ESCHBORN |
ISSN | 0031-7144 |
卷号 | 72期号:2页码:87-90 |
DOI | 10.1691/ph.2017.6839 |
页数 | 4 |
WOS类目 | Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
WOS研究方向 | Pharmacology & Pharmacy ; Chemistry |
WOS记录号 | WOS:000397353600004 |
收录类别 | SCIE ; SCOPUS |
URL | 查看原文 |
PubMed ID | 29441858 |
SCOPUSEID | 2-s2.0-85013231687 |
通讯作者地址 | [Huang W.P.]Department of Urology,Wenzhou Medical University,Wenzhou,325000,China |
Scopus学科分类 | Pharmaceutical Science |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/19024 |
专题 | 附属第一医院 附属第二医院 第二临床医学院、附属第二医院、育英儿童医院 第一临床医学院(信息与工程学院)、附属第一医院_麻醉科 |
通讯作者 | Huang W.P. |
作者单位 | 1.Department of Urology,Wenzhou Medical University,Wenzhou,China; 2.Department of Anesthesiology,Wenzhou Medical University,Wenzhou,China |
第一作者单位 | 温州医科大学 |
通讯作者单位 | 温州医科大学 |
第一作者的第一单位 | 温州医科大学 |
推荐引用方式 GB/T 7714 | Huang X.X.,Wang R.X.,Lin Q.,et al. Inhibitory effects of 2-methoxyestradiol on cell growth and invasion in human bladder cancer T-24 cells[J]. PHARMAZIE,2017,72(2):87-90. |
APA | Huang X.X.., Wang R.X.., Lin Q.., Chen W.., Pan Y.., ... & Huang W.P.. (2017). Inhibitory effects of 2-methoxyestradiol on cell growth and invasion in human bladder cancer T-24 cells. PHARMAZIE, 72(2), 87-90. |
MLA | Huang X.X.,et al."Inhibitory effects of 2-methoxyestradiol on cell growth and invasion in human bladder cancer T-24 cells".PHARMAZIE 72.2(2017):87-90. |
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