Network analysis of miRNA targeting m6A-related genes in patients with esophageal cancer

doi:10.7717/peerj.11893

科研成果详情

题名	Network analysis of miRNA targeting m6A-related genes in patients with esophageal cancer
作者	Li, Lili 1,2; Xie, Rongrong 2; Wei, Qichun 1
发表日期	2021-07-29
发表期刊	PEERJ 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Esophageal cancer HNRNPC N6-methyladenosine Overall survival Prognosis
其他关键词	SQUAMOUS-CELL CARCINOMA ; M(6)A RNA METHYLATION ; METTL3 PROMOTES ; MESENCHYMAL TRANSITION ; TUMOR-SUPPRESSOR ; PROLIFERATION ; MICRORNA ; IDENTIFICATION ; CONTRIBUTES ; VALIDATION
摘要	Background: We investigated the miRNA-m6A related gene network and identified a miRNA-based prognostic signature in patients with esophageal cancer using integrated genomic analysis. Methods: We obtained expression data for m6A-related genes and miRNAs from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets. Survival analysis was conducted to identify potential prognostic biomarkers. LASSO Cox regression was performed to construct the overall survival (OS) associated prediction signature. We used the Kaplan-Meier (K-M) curve and receiver operating characteristic (ROC) curves to explore the signature's efficiency and accuracy. Interactions between the m6A-related genes and miRNAs were identified in starBase3.0 and used to construct the miRNA-m6A related gene network. Results: We found that HNRNPC, YTHDF, ZC3H13, YTHDC2, and METTL14 were dysregulated in esophageal cancer tissues. Multivariate Cox regression analysis revealed that HNRNPC may be an independent risk factor for OS. Five hundred twenty-two potential upstream miRNAs were obtained from starBase3.0. Four miRNAs (miR-186, miR-320c, miR-320d, and miR-320b) were used to construct a prognostic signature, which could serve as a prognostic predictor independent from routine clinicopathological features. Finally, we constructed a key miRNA-m6A related gene network and used one m6A-related gene and four miRNAs associated with the prognosis. The results of our bioinformatics analysis were successfully validated in the human esophageal carcinoma cell lines KYSE30 and TE-1. Conclusion: Our study identified a 4-miRNA prognostic signature and established a key miRNA-m6A related gene network. These tools may reliably assist with esophageal cancer patient prognosis.
资助项目	Wenzhou Basic scientific research project of China [Y2020743]
出版者	PEERJ INC
出版地	LONDON
ISSN	2167-8359
卷号	9 页码:e11893.
DOI	10.7717/peerj.11893
页数	19
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000678776100005
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	34395102
PMC记录号	PMC8325912
SCOPUSEID	2-s2.0-85111652926
通讯作者地址	[Wei, Qichun]Department of Radiation Oncology,Second Affiliated Hospital,Zhejiang University,School of Medicine,Hangzhou,China
Scopus学科分类	Neuroscience (all);Biochemistry, Genetics and Molecular Biology (all);Agricultural and Biological Sciences (all)
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1898
专题	附属第一医院_肿瘤内科
通讯作者	Wei, Qichun
作者单位	1.Department of Radiation Oncology,Second Affiliated Hospital,Zhejiang University,School of Medicine,Hangzhou,China; 2.Department of Medical Oncology,First Affiliated Hospital of Wenzhou,Medical University,Wenzhou,China
推荐引用方式 GB/T 7714	Li, Lili,Xie, Rongrong,Wei, Qichun. Network analysis of miRNA targeting m6A-related genes in patients with esophageal cancer[J]. PEERJ,2021,9:e11893..
APA	Li, Lili, Xie, Rongrong, & Wei, Qichun. (2021). Network analysis of miRNA targeting m6A-related genes in patients with esophageal cancer. PEERJ, 9, e11893..
MLA	Li, Lili,et al."Network analysis of miRNA targeting m6A-related genes in patients with esophageal cancer".PEERJ 9(2021):e11893..