Robust analysis of novel mRNA-IncRNA cross talk based on ceRNA hypothesis uncovers carcinogenic mechanism and promotes diagnostic accuracy in esophageal cancer

doi:10.2147/CMAR.S183310

科研成果详情

题名	Robust analysis of novel mRNA-IncRNA cross talk based on ceRNA hypothesis uncovers carcinogenic mechanism and promotes diagnostic accuracy in esophageal cancer
作者	Chen, Li-Ping1,2; Wang, Hong1; Zhang, Yi 2; Chen, Qiu-Xiang3; Lin, Tie-Su4; Liu, Zong-Qin 5; Zhou, Yang-Yang1
发表日期	2019
发表期刊	CANCER MANAGEMENT AND RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
关键词	competitive endogenous RNA network esophagus cancer LASSO regression model diagnosis
其他关键词	MICRORNA TARGET PREDICTION ; COMPETING ENDOGENOUS RNAS ; LONG NONCODING RNA ; TUMOR-SUPPRESSOR ; GENE-EXPRESSION ; THYROID-CANCER ; LNCRNA ; MIRNAS ; BIOMARKERS ; MIGRATION
摘要	Background: ceRNAs have emerged as pivotal players in the regulation of gene expression and play a crucial role in the physiology and development of various cancers. Nevertheless, the function and underlying mechanisms of ceRNAs in esophageal cancer (EC) are still largely unknown. Methods: In this study, profiles of DEmRNAs, DEIncRNAs, and DEmiRNAs between normal and EC tumor tissue samples were obtained from the Cancer Genome Atlas database using the DESeq package in R by setting the adjusted P<0.05 and \|log(2) (fold change)\|>2 as the cutoff The ceRNA network (ceRNet) was initially constructed to reveal the interaction of these ceRNAs during carcinogenesis based on the bioinformatics of miRcode, miRDB, miRTarBase, and TargetScan. Then, independent microarray data of GSE6188, GSE89102, and GSE92396 and correlation analysis were used to validate molecular biomarkers in the initial ceRNet. Finally, a least absolute shrinkage and selection operator logistic regression model was built using an oncogenic ceRNet to diagnose EC more accurately. Results: We successfully constructed an oncogenic ceRNet of EC, crosstalk of hsa-miR372-centered CADM2-ADAMTS9-AS2 and hsa-miR145-centered SERPINE1-PVT1. In addition, the risk-score model -0.0053log(2)(CADM2)+0.0168log(2)(SERPINE/)-0.0073log(2)(ADAMTS9-AS2)+0.0905log(2)(PVT1)+0.0047log(2)(hsa-miR372)-0.0193log(2)(hsa-miR145), (log(2)[gene count]) could improve diagnosis of EC with an AUC of 0.988. Conclusion: We identified two novel pairs of ceRNAs in EC and its role of diagnosis. The pairs of hsa-miR372-centered CADM2-ADAMTS9-AS2 and hsa-miR145-centered SERPINE1-PVT1 were likely potential carcinogenic mechanisms of EC, and their joint detection could improve diagnostic accuracy.
出版者	DOVE MEDICAL PRESS LTD
出版地	ALBANY
ISSN	1179-1322
卷号	11 页码:347-358
DOI	10.2147/CMAR.S183310
页数	12
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000454528200004
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	30643460
PMC记录号	PMC6312067
SCOPUSEID	2-s2.0-85059448133
通讯作者地址	[Zhou, Yang-Yang]Department of Rheumatology and Immunology,Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,109 Xueyuan West Road, Lucheng,Wenzhou,325000,China
Scopus学科分类	Oncology
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/18855
专题	第二临床医学院，附属第二医院、育英儿童医院温州医科大学附属第一医院药学院（分析测试中心）_生物有机与药物化学研究中心
通讯作者	Zhou, Yang-Yang
作者单位	1.Department of Rheumatology and Immunology,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Chemical Biology Research Center,Department of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,325000,China; 3.Department of Ultrasonography,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 4.Department of Gastroenterology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 5.Department of Laboratory Medicine,Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 药学院（分析测试中心）_生物有机与药物化学研究中心
通讯作者单位	附属第二医院
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Chen, Li-Ping,Wang, Hong,Zhang, Yi,et al. Robust analysis of novel mRNA-IncRNA cross talk based on ceRNA hypothesis uncovers carcinogenic mechanism and promotes diagnostic accuracy in esophageal cancer[J]. CANCER MANAGEMENT AND RESEARCH,2019,11:347-358.
APA	Chen, Li-Ping., Wang, Hong., Zhang, Yi., Chen, Qiu-Xiang., Lin, Tie-Su., ... & Zhou, Yang-Yang. (2019). Robust analysis of novel mRNA-IncRNA cross talk based on ceRNA hypothesis uncovers carcinogenic mechanism and promotes diagnostic accuracy in esophageal cancer. CANCER MANAGEMENT AND RESEARCH, 11, 347-358.
MLA	Chen, Li-Ping,et al."Robust analysis of novel mRNA-IncRNA cross talk based on ceRNA hypothesis uncovers carcinogenic mechanism and promotes diagnostic accuracy in esophageal cancer".CANCER MANAGEMENT AND RESEARCH 11(2019):347-358.