Electro-acupuncture treatment improves neurological function associated with downregulation of PDGF and inhibition of astrogliosis in rats with spinal cord transection

doi:10.1007/s12031-013-0035-3

科研成果详情

题名	Electro-acupuncture treatment improves neurological function associated with downregulation of PDGF and inhibition of astrogliosis in rats with spinal cord transection
作者	Liu, Fen 1,2; Zou, Yu 3; Liu, Sujuan 4; Liu, Jia 3; Wang, Tinghua 2,3
发表日期	2013-10
发表期刊	Journal of molecular neuroscience : MN 影响因子和分区
语种	英语
原始文献类型	Journal Article ; Research Support, Non-U.S. Gov't
关键词	Electro-acupuncture Spinal cord injury PDGF Astrogliosis
其他关键词	GROWTH-FACTOR ; SCAR FORMATION ; ACUPUNCTURE ; ASTROCYTES ; EXPRESSION ; INJURY ; REPAIR ; CELLS
摘要	The aim of the current study was to determine the effects of electro-acupuncture (EA) on the improvement of locomotor function in injured spinal cord and underlying mechanism. Forty-five female Sprague-Dawley rats (180~200 g) were randomly divided into three groups, sham operation control group (sham), spinal cord transection group (SCT) and EA group. The Basso, Beattie, and Bresnahan (BBB) Locomotor Rating Scale was used to evaluate functional recovery of rats in hindlimbs at 1, 3, 5 weeks after injury and EA therapy. The gene and protein expression of glial fibrillary acidic protein (GFAP) and platelet derived growth factor (PDGF) were measured by reverse transcription-polymerase chain reaction and Western blot analysis, respectively, and the number of GFAP-positive cells was counted, also. Compared with SCT group, the locomotor function in hindlimbs of rats was improved after 1, 3, 5 weeks following EA therapy. EA treatment not only decreased effectively the number of GFAP immunostaining and GFAP expression, but also downregulated the PDGF expression both gene and protein, in addition decreased the number of PDGF immunostaining in injured spinal cord of rats with transection. It therefore concluded that EA therapy can significantly promote the recovery of locomotor function, and this may be linked to the inhibition of astrogliosis, together with the downregulation of PDGF.
资助项目	Zhejiang Provincial Natural Science Foundation of China [LQ12H09003]; Wenzhou City Science and Technology Plan Projects of China [Y20110040]
出版者	HUMANA PRESS INC
ISSN	0895-8696
EISSN	1559-1166
卷号	51 期号:2 页码:629-635
DOI	10.1007/s12031-013-0035-3
页数	7
WOS类目	Biochemistry & Molecular Biology ; Neurosciences
WOS研究方向	Biochemistry & Molecular Biology ; Neurosciences & Neurology
WOS记录号	WOS:000324637200048
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	23749676
SCOPUSEID	2-s2.0-84885094003
通讯作者地址	[Wang, Tinghua]Translational Neuroscience Center,West China Hospital,Sichuan University,China
Scopus学科分类	Cellular and Molecular Neuroscience
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/186012
专题	基础医学院（机能实验教学中心）_形态学系_组织胚胎学
通讯作者	Wang, Tinghua
作者单位	1.Department of Histology and Embryology,Institute of Neuroscience,Wenzhou Medical College,China; 2.Translational Neuroscience Center,West China Hospital,Sichuan University,China; 3.Institute of Neuroscience,Kunming Medical University,China; 4.Department of Histology and Neurobiology,West China School of Preclinical and Forensic Medicine,China
第一作者单位	基础医学院（机能实验教学中心）_形态学系_组织胚胎学
第一作者的第一单位	基础医学院（机能实验教学中心）_形态学系_组织胚胎学
推荐引用方式 GB/T 7714	Liu, Fen,Zou, Yu,Liu, Sujuan,et al. Electro-acupuncture treatment improves neurological function associated with downregulation of PDGF and inhibition of astrogliosis in rats with spinal cord transection[J]. Journal of molecular neuroscience : MN,2013,51(2):629-635.
APA	Liu, Fen, Zou, Yu, Liu, Sujuan, Liu, Jia, & Wang, Tinghua. (2013). Electro-acupuncture treatment improves neurological function associated with downregulation of PDGF and inhibition of astrogliosis in rats with spinal cord transection. Journal of molecular neuroscience : MN, 51(2), 629-635.
MLA	Liu, Fen,et al."Electro-acupuncture treatment improves neurological function associated with downregulation of PDGF and inhibition of astrogliosis in rats with spinal cord transection".Journal of molecular neuroscience : MN 51.2(2013):629-635.