Determination of morphine, codeine, thebaine, papaverine and noscapine in rat plasma by UPLC-MS/MS

doi:10.1556/1326.2023.01108

科研成果详情

题名	Determination of morphine, codeine, thebaine, papaverine and noscapine in rat plasma by UPLC-MS/MS
作者	Wang, Ying 1; He, Yifan2; Wang, Xianqin3; Wen, Congcong 2; Cao, Jianbin4
发表日期	2023
发表期刊	Acta Chromatographica 影响因子和分区
语种	英语
原始文献类型	Article
关键词	codeine morphine noscapine papaverine plasma thebaine UPLC-MS/MS
其他关键词	PERICARPIUM PAPAVERIS ; CHROMATOGRAPHY ; PURIFICATION ; EXTRACTION ; ONLINE ; SYSTEM
摘要	In this work, a UPLC-MS/MS assay was established for the determination of morphine, codeine, thebaine, papaverine and noscapine in rat plasma. ACQUITY UPLC BEH C18 column was employed for chromatographic separation with the mobile phase comprised acetonitrile-10 mmol L-1 ammonium acetate aqueous solution (0.05% aqueous ammonia) using gradient elution. Midazolam was used as internal standard (IS). Electrospray ionization (ESI) in positive-ion mode with reaction monitoring (MRM) was used for quantitative analysis. The calibration curves for morphine, codeine, thebaine, papaverine and noscapine demonstrated good linearity (r > 0.995) in the range of 5-500 ng mL-1 for morphine and codeine, and 1-100 ng mL-1 for thebaine, papaverine and noscapine. The intra-day and inter-day precisions of morphine, codeine, thebaine, papaverine and noscapine were within 15%, the intra-day and inter-day accuracies were 89-114%, the recovery was better than 65%, and the matrix effects were 96-112%. The developed UPLC-MS/MS assay was successfully applied in the pharmacokinetics of papaverine and noscapine.
资助项目	Ningbo Natural Science Foundation [202003N4265]; Basic Public Welfare Research Project of Zhejiang Province [LGD20H090005]; Medical Science and Technology Project of Zhejiang Province [2022RC293]
出版者	AKADEMIAI KIADO ZRT
ISSN	2083-5736
EISSN	2083-5736
卷号	36 期号:3 页码:179-185
DOI	10.1556/1326.2023.01108
页数	7
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
WOS记录号	WOS:001308771100001
收录类别	SCOPUS ; SCIE
URL	查看原文
SCOPUSEID	2-s2.0-85156175456
通讯作者地址	[Wen, Congcong]Laboratory Animal Center,Wenzhou Medical University,Wenzhou,China ; [Cao, Jianbin]Department of Anesthesiology,Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University,Taizhou,China
Scopus学科分类	Chemistry (all)
SCOPUS_ID	SCOPUS_ID:85156175456
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/184455
专题	实验动物中心药学院（分析测试中心）附属台州医院其他_附属台州医院（浙江省台州医院）
通讯作者	Wen, Congcong; Cao, Jianbin
作者单位	1.Pharmacy Department,Ningbo Medical Treatment Center Lihuili Hospital,Ningbo,China; 2.Laboratory Animal Center,Wenzhou Medical University,Wenzhou,China; 3.Analytical and Testing Center,School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China; 4.Department of Anesthesiology,Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University,Taizhou,China
通讯作者单位	实验动物中心; 附属台州医院（浙江省台州医院）; 附属台州医院
推荐引用方式 GB/T 7714	Wang, Ying,He, Yifan,Wang, Xianqin,et al. Determination of morphine, codeine, thebaine, papaverine and noscapine in rat plasma by UPLC-MS/MS[J]. Acta Chromatographica,2023,36(3):179-185.
APA	Wang, Ying, He, Yifan, Wang, Xianqin, Wen, Congcong, & Cao, Jianbin. (2023). Determination of morphine, codeine, thebaine, papaverine and noscapine in rat plasma by UPLC-MS/MS. Acta Chromatographica, 36(3), 179-185.
MLA	Wang, Ying,et al."Determination of morphine, codeine, thebaine, papaverine and noscapine in rat plasma by UPLC-MS/MS".Acta Chromatographica 36.3(2023):179-185.