Gelatin Nanostructured Lipid Carriers Incorporating Nerve Growth Factor Inhibit Endoplasmic Reticulum Stress-Induced Apoptosis and Improve Recovery in Spinal Cord Injury

doi:10.1007/s12035-015-9372-2

科研成果详情

题名	Gelatin Nanostructured Lipid Carriers Incorporating Nerve Growth Factor Inhibit Endoplasmic Reticulum Stress-Induced Apoptosis and Improve Recovery in Spinal Cord Injury
作者	Zhu, Si-Pin1,2; Wang, Zhou-Guang 2; Zhao, Ying-Zheng2; Wu, Jiang2; Shi, Hong-Xue2; Ye, Li-Bing 2; Wu, Fen-Zan 3; Cheng, Yi 2; Zhang, Hong-Yu2; He, Songbin 4; Wei, Xiaojie3; Fu, Xiao-Bing 5; Li, Xiao-Kun2; Xu, Hua-Zi1,2; Xiao, Jian2
发表日期	2016-09
发表期刊	MOLECULAR NEUROBIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Gelatin nanostructured lipid carriers NGF ER stress Spinal cord injury
其他关键词	UNFOLDED PROTEIN RESPONSE ; ENHANCES FUNCTIONAL RECOVERY ; CELL-DEATH ; REDUCES APOPTOSIS ; RAT ; MODEL ; ISCHEMIA ; NEURONS ; TRAUMA ; NANOPARTICLES
摘要	Clinical translation of growth factor therapies faces multiple challenges; the most significant one is the short half-life of the naked protein. Gelatin nanostructured lipid carriers (GNLs) had previously been used to encapsulate the basic fibroblast growth factor to enhance the functional recovery in hemiparkinsonian rats. In this research, we comparatively study the enhanced therapy between nerve growth factor (NGF) loaded GNLs (NGF-GNLs) and NGF only in spinal cord injury (SCI). The effects of NGF-GNLs and NGF only were tested by the Basso-Beattie-Bresnahan (BBB) locomotion scale, inclined plane test, and footprint analysis. Western blot analysis and immunofluorescent staining were further performed to identify the expression of ER stress-related proteins, neuron-specific marker neuronal nuclei (NeuN), and growth-associated protein 43 (GAP43). Correlated downstream signals Akt/GSK-3 beta and ERK1/2 were also analyzed with or without inhibitors. Results showed that NGF-GNLs, compared to NGF only, enhanced the neuroprotection effect in SCI rats. The ER stress-induced apoptosis response proteins CHOP, GRP78 and caspase-12 inhibited by NGF-GNL treatment were more obvious. Meanwhile, NGF-GNLs in the recovery of SCI are related to the inhibition of ER stress-induced cell death via the activation of downstream signals PI3K/Akt/GSK-3 beta and ERK1/2.
资助项目	Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health talents; National Natural Science Funding of ChinaNational Natural Science Foundation of China (NSFC) [81372112, 81200958, 81302775]; Zhejiang Natural Science Funding [Y14H170008]; State Key Basic Research Development Program [2012CB518105]; Ningbo Natural Science Foundation [2012A610255]; Zhejiang undergraduate talent project [2014R413067]
出版者	HUMANA PRESS INC
出版地	TOTOWA
ISSN	0893-7648
EISSN	1559-1182
卷号	53 期号:7 页码:4375-4386
DOI	10.1007/s12035-015-9372-2
页数	12
WOS类目	Neurosciences
WOS研究方向	Neurosciences & Neurology
WOS记录号	WOS:000380678600009
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	26232067
SCOPUSEID	2-s2.0-84938614215
通讯作者地址	[Xu, Hua-Zi]Department of Orthopaedics,The Second Affiliated Hospital,Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Neurology;Cellular and Molecular Neuroscience
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1840
专题	附属第二医院温州医科大学药学院（分析测试中心）_分子药理研究与教学中心其他_附属舟山医院（舟山医院）
通讯作者	Xu, Hua-Zi
作者单位	1.Department of Orthopaedics,The Second Affiliated Hospital,Wenzhou Medical University,Wenzhou,325000,China; 2.Molecular Pharmacology Research Center,School of Pharmacy,Wenzhou Medical University,Wenzhou,325035,China; 3.Department of Neurosurgery,Cixi People’s Hospital,Wenzhou Medical University,Ningbo,315300,China; 4.Department of Neurology,Zhoushan Hospital,Wenzhou Medical University,Zhoushan,316021,China; 5.Institute of Basic Medical Science,Chinese PLA General Hospital,Beijing,100853,China
第一作者单位	附属第二医院; 药学院（分析测试中心）_分子药理研究与教学中心
通讯作者单位	附属第二医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Zhu, Si-Pin,Wang, Zhou-Guang,Zhao, Ying-Zheng,et al. Gelatin Nanostructured Lipid Carriers Incorporating Nerve Growth Factor Inhibit Endoplasmic Reticulum Stress-Induced Apoptosis and Improve Recovery in Spinal Cord Injury[J]. MOLECULAR NEUROBIOLOGY,2016,53(7):4375-4386.
APA	Zhu, Si-Pin., Wang, Zhou-Guang., Zhao, Ying-Zheng., Wu, Jiang., Shi, Hong-Xue., ... & Xiao, Jian. (2016). Gelatin Nanostructured Lipid Carriers Incorporating Nerve Growth Factor Inhibit Endoplasmic Reticulum Stress-Induced Apoptosis and Improve Recovery in Spinal Cord Injury. MOLECULAR NEUROBIOLOGY, 53(7), 4375-4386.
MLA	Zhu, Si-Pin,et al."Gelatin Nanostructured Lipid Carriers Incorporating Nerve Growth Factor Inhibit Endoplasmic Reticulum Stress-Induced Apoptosis and Improve Recovery in Spinal Cord Injury".MOLECULAR NEUROBIOLOGY 53.7(2016):4375-4386.