Genomic and Phylogenetic Analysis of a Multidrug-Resistant blaNDM-carrying Klebsiella michiganensis in China

doi:10.2147/IDR.S409544

科研成果详情

题名	Genomic and Phylogenetic Analysis of a Multidrug-Resistant blaNDM-carrying Klebsiella michiganensis in China
作者	Jiang, Tian1; Li, Guoli 2; Huang, Linyao1; Ding, Ding 1; Ruan, Zhi 2; Yan, Jianxin 1
发表日期	2023
发表期刊	INFECTION AND DRUG RESISTANCE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	whole-genome sequencing Klebsiella michiganensis bla NDM multidrug-resistant phylogenetics
其他关键词	GENES ; ENTEROBACTERIACEAE ; BLA(KPC-2) ; PNEUMONIAE ; PLASMID
摘要	Objective: Klebsiella michiganensis is an emerging hospital-acquired bacterial pathogen. However, there is a dearth of knowledge on the antimicrobial resistance and transmission of K. michiganensis. Here, we characterized the microbiological and genomic features of a carbapenem-resistant K. michiganensis strain harboring the blaNDM-1 gene in China.Methods: K. michiganensis strain 2563 was recovered from the sputum sample of a hospitalized patient with pulmonary infection. Whole-genome sequencing of K. michiganensis strain 2563 was conducted using both the short-read Illumina and long-read MinION platforms to thoroughly characterize the genetic context of blaNDM-carrying plasmid in K. michiganensis 2563. Furthermore, BacWGSTdb server was utilized to perform in silico multilocus sequence typing (MLST), identify antimicrobial resistance genes, and conduct genomic epidemiological analyses of the closely related isolates deposited in the public database.Results: K. michiganensis 2563 was resistant to piperacillin, aztreonam, meropenem, imipenem, amoxicillin-clavulanic acid, ampicillin, cefotaxime, cefazolin, ampicillin/sulbactam, cefepime, piperacillin-tazobactam, and ceftazidime. It belonged to sequence type (ST) 43, and the blaNDM-1 gene was found to be located on the plasmid p2563_NDM (54,035 bp). This plasmid showed remarkable similarity to other blaNDM-1-encoding plasmids found in various Enterobacterium species in the public database. The occurrence of global ST43 K. michiganensis was primarily sporadic, and the closest relative of K. michiganensis 2563 was another ST43 isolate 12,084 recovered from China in 2013, which differed by 171 SNPs.Conclusion: Our study reports the genome characteristics of a carbapenem-resistant K. michiganensis strain carrying the blaNDM-1 gene in China, highlighting the need for ongoing surveillance of this pathogen in clinical settings.
资助项目	Zhejiang Provincial Medical and Health Science and Technology plan [2022RC299, 2023KY119, 2023XY088]
出版者	DOVE MEDICAL PRESS LTD
ISSN	1178-6973
卷号	16 页码:3109-3116
DOI	10.2147/IDR.S409544
页数	8
WOS类目	Infectious Diseases ; Pharmacology & Pharmacy
WOS研究方向	Infectious Diseases ; Pharmacology & Pharmacy
WOS记录号	WOS:000994615200001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	3722866
SCOPUSEID	2-s2.0-85160677510
通讯作者地址	[Ruan, Zhi]Department of Clinical Laboratory,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou,China ; [Yan, Jianxin]Department of Clinical Laboratory,The Affiliated Wenling Hospital,Wenzhou Medical University,Wenling,China
Scopus学科分类	Pharmacology;Infectious Diseases;Pharmacology (medical)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/183992
专题	温州医科大学
通讯作者	Ruan, Zhi; Yan, Jianxin
作者单位	1.Department of Clinical Laboratory,The Affiliated Wenling Hospital,Wenzhou Medical University,Wenling,China; 2.Department of Clinical Laboratory,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou,China
推荐引用方式 GB/T 7714	Jiang, Tian,Li, Guoli,Huang, Linyao,et al. Genomic and Phylogenetic Analysis of a Multidrug-Resistant blaNDM-carrying Klebsiella michiganensis in China[J]. INFECTION AND DRUG RESISTANCE,2023,16:3109-3116.
APA	Jiang, Tian, Li, Guoli, Huang, Linyao, Ding, Ding, Ruan, Zhi, & Yan, Jianxin. (2023). Genomic and Phylogenetic Analysis of a Multidrug-Resistant blaNDM-carrying Klebsiella michiganensis in China. INFECTION AND DRUG RESISTANCE, 16, 3109-3116.
MLA	Jiang, Tian,et al."Genomic and Phylogenetic Analysis of a Multidrug-Resistant blaNDM-carrying Klebsiella michiganensis in China".INFECTION AND DRUG RESISTANCE 16(2023):3109-3116.