Effects of metalloprotease ADAMTS12 on cervical cancer cell phenotype and its potential mechanism

doi:10.1007/s12672-023-00776-2

科研成果详情

题名	Effects of metalloprotease ADAMTS12 on cervical cancer cell phenotype and its potential mechanism
作者	Zou, Ruanmin1; Gu, Ruihong 2; Tu, Xinyu 2; Chen, Jiani2; Liu, Songjun 3; Xue, Xiangyang2; Li, Wensu 2; Zhang, Yuyang1
发表日期	2023-08-29
发表期刊	Discover. Oncology 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	ADAMTS12 Cervical cancer Invasion Migration TGF-beta
其他关键词	TGF-BETA ; GROWTH ; IDENTIFICATION ; DEGRADATION ; DISINTEGRIN ; INHIBITION ; EXPRESSION ; GENE
摘要	ADAMTS12 is a gene widely expressed in human tissues. We studied the expression level of ADAMTS12 in cervical cancer tissue and its relationship with clinicopathological features. We also explored the function of ADAMTS12 in cervical cancer cells and its underlying mechanisms. We found the higher expression level of ADAMTS12 in cancer tissues, which was associated with the worse overall survival rate. The immunofluorescence assay showed that the cytoplasm of cervical cancer cells is the main expression site of ADAMTS12. Overexpression of ADAMTS12 in HeLa and CaSki cells prominently promoted the cell proliferation, migration and invasion. We found that 2032 genes were correlated with ADAMTS12, which was mainly related to extracellular matrix, TGF-β signaling pathway. The phosphorylation levels of mTOR and 4E-BP1 were upregulated in ADAMTS12-overexpressing cells. Co-Immunoprecipitation combined with protein mass spectrometry showed that TGF-β signaling pathway-related proteins interacting with ADAMTS12 were screened from HeLa cells with ADAMTS12 overexpression. Therefore, we concluded that ADAMTS12 may affect the mTOR signaling pathway through the interacting with TGF-β1, and then affect the biological function of cervical cancer cells.
资助项目	We thank college of Basic Medicine of Wenzhou Medical University for support on this manuscript.; college of Basic Medicine of Wenzhou Medical University
出版者	SPRINGER
ISSN	2730-6011
EISSN	2730-6011
卷号	14 期号:1
DOI	10.1007/s12672-023-00776-2
页数	17
WOS类目	Oncology ; Endocrinology & Metabolism
WOS研究方向	Oncology ; Endocrinology & Metabolism
WOS记录号	WOS:001057894300001
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	37642715
SCOPUSEID	2-s2.0-85169035142
通讯作者地址	[Zou, Ruanmin]Department of Obstetrics and Gynecology,The First Affiliated Hospital,Wenzhou Medical University,Wenzhou,China ; [Zhang, Yuyang]Department of Obstetrics and Gynecology,The First Affiliated Hospital,Wenzhou Medical University,Wenzhou,China ; [Li, Wensu]Department of Microbiology and Immunology,Institute of Molecular Virology and Immunology,Institute of Tropical Medicine,College of Basic Medicine,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Endocrinology, Diabetes and Metabolism;Oncology;Endocrinology;Endocrine and Autonomic Systems;Cancer Research
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/182526
专题	附属第一医院基础医学院（机能实验教学中心）检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系
通讯作者	Zou, Ruanmin; Li, Wensu; Zhang, Yuyang
作者单位	1.Department of Obstetrics and Gynecology,The First Affiliated Hospital,Wenzhou Medical University,Wenzhou,China; 2.Department of Microbiology and Immunology,Institute of Molecular Virology and Immunology,Institute of Tropical Medicine,College of Basic Medicine,Wenzhou Medical University,Wenzhou,China; 3.Department of Gynecology,Tongde Hospital of Zhejiang Province,Hangzhou,China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院; 基础医学院（机能实验教学中心）; 病原生物学与免疫学系
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Zou, Ruanmin,Gu, Ruihong,Tu, Xinyu,et al. Effects of metalloprotease ADAMTS12 on cervical cancer cell phenotype and its potential mechanism[J]. Discover. Oncology,2023,14(1).
APA	Zou, Ruanmin., Gu, Ruihong., Tu, Xinyu., Chen, Jiani., Liu, Songjun., ... & Zhang, Yuyang. (2023). Effects of metalloprotease ADAMTS12 on cervical cancer cell phenotype and its potential mechanism. Discover. Oncology, 14(1).
MLA	Zou, Ruanmin,et al."Effects of metalloprotease ADAMTS12 on cervical cancer cell phenotype and its potential mechanism".Discover. Oncology 14.1(2023).