VGLL4 promotes vascular endothelium specification via TEAD1 in the vascular organoids and human pluripotent stem cells-derived endothelium model

doi:10.1007/s00018-023-04858-w

科研成果详情

题名	VGLL4 promotes vascular endothelium specification via TEAD1 in the vascular organoids and human pluripotent stem cells-derived endothelium model
作者	Quan, Yingyi 1,2; Hu, Minjie1; Jiang, Jiang 2; Jin, Peifeng3; Fan, Junming1; Li, Ming4; Fan, Xiaofang1; Gong, Yongsheng1; Yang, Yang 2; Wang, Yongyu1,4
发表日期	2023-08
发表期刊	CELLULAR AND MOLECULAR LIFE SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	VGLL4 TEAD1 Vascular Organoids Human pluripotent stem cells-derived endothelial cells FLI1
其他关键词	TUMOR-SUPPRESSOR ; YAP ; GENERATION
摘要	BackgroundWe have shown that Hippo-YAP signaling pathway plays an important role in endothelial cell differentiation. Vestigial-like family member 4 (VGLL4) has been identified as a YAP inhibitor. However, the exact function of VGLL4 in vascular endothelial cell development remains unclear. In this study, we investigated the role of VGLL4, in human endothelial lineage specification both in 3D vascular organoid and 2D endothelial cell differentiation.Methods and resultsIn this study, we found that VGLL4 was increased during 3D vascular organoids generation and directed differentiation of human embryonic stem cells H1 towards the endothelial lineage. Using inducible ectopic expression of VGLL4 based on the piggyBac system, we proved that overexpression of VGLL4 in H1 promoted vascular organoids generation and endothelial cells differentiation. In contrast, VGLL4 knockdown (heterozygous knockout) of H1 exhibited inhibitory effects. Using bioinformatics analysis and protein immunoprecipitation, we further found that VGLL4 binds to TEAD1 and facilitates the expression of endothelial master transcription factors, including FLI1, to promote endothelial lineage specification. Moreover, TEAD1 overexpression rescued VGLL4 knockdown-mediated negative effects.ConclusionsIn summary, VGLL4 promotes EC lineage specification both in 3D vascular organoid and 2D EC differentiation from pluripotent stem cell, VGLL4 interacts with TEAD1 and facilitates EC key transcription factor, including FLI1, to enhance EC lineage specification.
资助项目	national key research and development program of china[2019YFA0802700];National Natural Science Foundation of China[82070487];National Natural Science Foundation of China[81670454];national natural science foundation of china[32070804];Natural Science Foundation of Zhejiang Province[LY21C120003];scientific research start-up fund of wenzhou medical university[QTJ15029]
出版者	SPRINGER BASEL AG
ISSN	1420-682X
EISSN	1420-9071
卷号	80 期号:8
DOI	10.1007/s00018-023-04858-w
页数	16
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:001033518400003
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	37468661
SCOPUSEID	2-s2.0-85165324453
通讯作者地址	[Wang, Yongyu]Institute of Hypoxia Medicine,School of Basic Medical Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China ; [Yang, Yang]State Key Laboratory of Reproductive Medicine and Offspring Health,Nanjing Medical University,Jiangsu,Nanjing,211116,China
Scopus学科分类	Molecular Medicine;Molecular Biology;Pharmacology;Cellular and Molecular Neuroscience;Cell Biology
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/181622
专题	基础医学院（机能实验教学中心）第一临床医学院（信息与工程学院）、附属第一医院附属第一医院
通讯作者	Yang, Yang; Wang, Yongyu
作者单位	1.Institute of Hypoxia Medicine,School of Basic Medical Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China; 2.State Key Laboratory of Reproductive Medicine and Offspring Health,Nanjing Medical University,Jiangsu,Nanjing,211116,China; 3.Department of Cardiothoracic Surgery,The First Affiliated Hospital,Wenzhou Medical University,Zhejiang,Wenzhou,325015,China; 4.Cardiac Regeneration Research Institute,School of Basic Medical Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China
第一作者单位	基础医学院（机能实验教学中心）
通讯作者单位	基础医学院（机能实验教学中心）
第一作者的第一单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Quan, Yingyi,Hu, Minjie,Jiang, Jiang,et al. VGLL4 promotes vascular endothelium specification via TEAD1 in the vascular organoids and human pluripotent stem cells-derived endothelium model[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2023,80(8).
APA	Quan, Yingyi., Hu, Minjie., Jiang, Jiang., Jin, Peifeng., Fan, Junming., ... & Wang, Yongyu. (2023). VGLL4 promotes vascular endothelium specification via TEAD1 in the vascular organoids and human pluripotent stem cells-derived endothelium model. CELLULAR AND MOLECULAR LIFE SCIENCES, 80(8).
MLA	Quan, Yingyi,et al."VGLL4 promotes vascular endothelium specification via TEAD1 in the vascular organoids and human pluripotent stem cells-derived endothelium model".CELLULAR AND MOLECULAR LIFE SCIENCES 80.8(2023).