Characterization of microbial community and antibiotic resistome in intra urban water, Wenzhou China

doi:10.3389/fmicb.2023.1169476

科研成果详情

题名	Characterization of microbial community and antibiotic resistome in intra urban water, Wenzhou China
作者	Ye, Sheng1; Li, Shengkai1; Su, Chenjun 2; Shi, Zhuqing1; Li, Heng 2; Hong, Jiawen1,3; Wang, Shengke1; Zhao, Jingyan 1; Zheng, Weiji 1; Dong, Shixuan 1; Ye, Shuhan 1; Lou, Yongliang1; Zhou, Zhemin 2; Du, Jimei1
发表日期	2023-06-15
发表期刊	FRONTIERS IN MICROBIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	waterborne pathogen antibiotic resistance metagenomic analysis public health environment
其他关键词	BETA-LACTAMASE ESBL ; RESISTANCE GENES ; WASTE-WATER ; BACTERIA ; PREVALENCE ; REMOVAL ; SLUDGE ; ARGS
摘要	The present study investigated the water quality index, microbial composition and antimicrobial resistance genes in urban water habitats. Combined chemicals testing, metagenomic analyses and qualitative PCR (qPCR) were conducted on 20 locations, including rivers from hospital surrounds (n = 7), community surrounds (n = 7), and natural wetlands (n = 6). Results showed that the indexes of total nitrogen, phosphorus, and ammonia nitrogen of hospital waters were 2-3 folds high than that of water from wetlands. Bioinformatics analysis revealed a total of 1,594 bacterial species from 479 genera from the three groups of water samples. The hospital-related samples had the greatest number of unique genera, followed by those from wetlands and communities. The hospital-related samples contained a large number of bacteria associated with the gut microbiome, including Alistipes, Prevotella, Klebsiella, Escherichia, Bacteroides, and Faecalibacterium, which were all significantly enriched compared to samples from the wetlands. Nevertheless, the wetland waters enriched bacteria from Nanopelagicus, Mycolicibacterium and Gemmatimonas, which are typically associated with aquatic environments. The presence of antimicrobial resistance genes (ARGs) that were associated with different species origins in each water sample was observed. The majority of ARGs from hospital-related samples were carried by bacteria from Acinetobacter, Aeromonas and various genera from Enterobacteriaceae, which each was associated with multiple ARGs. In contrast, the ARGs that were exclusively in samples from communities and wetlands were carried by species that encoded only 1 to 2 ARGs each and were not normally associated with human infections. The qPCR showed that water samples of hospital surrounds had higher concentrations of intI1 and antimicrobial resistance genes such as tetA, ermA, ermB, qnrB, sul1, sul2 and other beta-lactam genes. Further genes of functional metabolism reported that the enrichment of genes associated with the degradation/utilization of nitrate and organic phosphodiester were detected in water samples around hospitals and communities compared to those from wetlands. Finally, correlations between the water quality indicators and the number of ARGs were evaluated. The presence of total nitrogen, phosphorus, and ammonia nitrogen were significantly correlated with the presence of ermA and sul1. Furthermore, intI1 exhibited a significant correlation with ermB, sul1, and bla(SHV), indicating a prevalence of ARGs in urban water environments might be due to the integron intI1's diffusion-promoting effect. However, the high abundance of ARGs was limited to the waters around the hospital, and we did not observe the geographical transfer of ARGs along with the river flow. This may be related to water purifying capacity of natural riverine wetlands. Taken together, continued surveillance is required to assess the risk of bacterial horizontal transmission and its potential impact on public health in the current region.
资助项目	Zhejiang Provincial Natural Science Foundation of China[LGF18C050002];Zhejiang Medical and Health Science and Technology Project[2018PY030];
出版者	FRONTIERS MEDIA SA
ISSN	1664-302X
EISSN	1664-302X
卷号	14
DOI	10.3389/fmicb.2023.1169476
页数	13
WOS类目	Microbiology
WOS研究方向	Microbiology
WOS记录号	WOS:001016399400001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	37396356
SCOPUSEID	2-s2.0-85163789503
通讯作者地址	[Lou, Yongliang]Wenzhou Key Laboratory of Sanitary Microbiology,Department of Microbiology and Immunology,School of Laboratory Medicine,Wenzhou Medical University,Wenzhou,China ; [Du, Jimei]Wenzhou Key Laboratory of Sanitary Microbiology,Department of Microbiology and Immunology,School of Laboratory Medicine,Wenzhou Medical University,Wenzhou,China ; [Zhou, Zhemin]Pasteurien College,Suzhou Medical College,Soochow University,Jiangsu,Suzhou,China
Scopus学科分类	Microbiology;Microbiology (medical)
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/181060
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Lou, Yongliang; Zhou, Zhemin; Du, Jimei
作者单位	1.Wenzhou Key Laboratory of Sanitary Microbiology,Department of Microbiology and Immunology,School of Laboratory Medicine,Wenzhou Medical University,Wenzhou,China; 2.Pasteurien College,Suzhou Medical College,Soochow University,Jiangsu,Suzhou,China; 3.Taizhou Hospital of Zhejiang Province,Taizhou,China
第一作者单位	基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者单位	基础医学院（机能实验教学中心）_病原生物学与免疫学系
第一作者的第一单位	基础医学院（机能实验教学中心）_病原生物学与免疫学系
推荐引用方式 GB/T 7714	Ye, Sheng,Li, Shengkai,Su, Chenjun,et al. Characterization of microbial community and antibiotic resistome in intra urban water, Wenzhou China[J]. FRONTIERS IN MICROBIOLOGY,2023,14.
APA	Ye, Sheng., Li, Shengkai., Su, Chenjun., Shi, Zhuqing., Li, Heng., ... & Du, Jimei. (2023). Characterization of microbial community and antibiotic resistome in intra urban water, Wenzhou China. FRONTIERS IN MICROBIOLOGY, 14.
MLA	Ye, Sheng,et al."Characterization of microbial community and antibiotic resistome in intra urban water, Wenzhou China".FRONTIERS IN MICROBIOLOGY 14(2023).