Engineering Bioactive Self-Healing Antibacterial Exosomes Hydrogel for Promoting Chronic Diabetic Wound Healing and Complete Skin Regeneration

doi:10.7150/thno.29766

科研成果详情

题名	Engineering Bioactive Self-Healing Antibacterial Exosomes Hydrogel for Promoting Chronic Diabetic Wound Healing and Complete Skin Regeneration
作者	Wang, Chenggui1,2; Wang, Min 3; Xu, Tianzhen 1,2; Zhang, Xingxing 4; Lin, Cai4; Gao, Weiyang1,2; Xu, Huazi1,2; Lei, Bo 3,5,6; Mao, Cong1,2
发表期刊	THERANOSTICS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	multifunctional hydrogel bioactive exosomes responsive sustained release diabetic wound healing
其他关键词	MESENCHYMAL STEM-CELLS ; MYOGENIC DIFFERENTIATION ; DELIVERY ; ANGIOGENESIS ; BACTERIA ; NANOPARTICLES ; PROLIFERATION ; MICROSPHERES ; ENHANCE ; SURFACE
摘要	Rationale: Chronic nonhealing diabetic wound therapy and complete skin regeneration remains a critical clinical challenge. The controlled release of bioactive factors from a multifunctional hydrogel was a promising strategy to repair chronic wounds. Methods: Herein, for the first time, we developed an injectable, self-healing and antibacterial polypeptide-based FHE hydrogel (F127/OHA-EPL) with stimuli-responsive adipose-derived mesenchymal stem cells exosomes (AMSCs-exo) release for synergistically enhancing chronic wound healing and complete skin regeneration. The materials characterization, antibacterial activity, stimulated cellular behavior and in vivo full-thickness diabetic wound healing ability of the hydrogels were performed and analyzed. Results: The FHE hydrogel possessed multifunctional properties including fast self-healing process, shear-thinning injectable ability, efficient antibacterial activity, and long term pH-responsive bioactive exosomes release behavior. In vitro, the FHE@exosomes (FHE@exo) hydrogel significantly promoted the proliferation, migration and tube formation ability of human umbilical vein endothelial cells (HUVECs). In vivo, the FHE@exo hydrogel significantly enhanced the healing efficiency of diabetic full-thickness cutaneous wounds, characterized with enhanced wound closure rates, fast angiogenesis, re-epithelization and collagen deposition within the wound site. Moreover, the FHE@exo hydrogel displayed better healing outcomes than those of exosomes or FHE hydrogel alone, suggesting that the sustained release of exosomes and FHE hydrogel can synergistically facilitate diabetic wound healing. Skin appendages and less scar tissue also appeared in FHE@exo hydrogel treated wounds, indicating its potent ability to achieve complete skin regeneration. Conclusion: This work offers a new approach for repairing chronic wounds completely through a multifunctional hydrogel with controlled exosomes release.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [51502237, 51802227, 51872224]; Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LGF18H150008]; Medical Health Science and Technology project of Zhejiang Province [2019315934]; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University [2018LHM-KFKT004]
出版者	IVYSPRING INT PUBL
出版地	LAKE HAVEN
ISSN	1838-7640
卷号	9 期号:1 页码:65-76
DOI	10.7150/thno.29766
页数	12
WOS类目	Medicine, Research & Experimental
WOS研究方向	Research & Experimental Medicine
WOS记录号	WOS:000452508600006
收录类别	SCIE ; PUBMED ; SCOPUS
发表日期	2019
URL	查看原文
Pubmed记录号	30662554
PMC记录号	PMC6332800
Scopus记录号	2-s2.0-85059524493
ESI高被引论文	2020-09 ; 2020-11 ; 2021-01 ; 2021-03 ; 2021-05 ; 2021-07 ; 2021-11 ; 2022-01 ; 2022-03 ; 2022-07 ; 2022-09 ; 2022-11 ; 2023-01 ; 2023-03 ; 2023-05 ; 2023-07 ; 2023-09 ; 2023-11 ; 2024-01 ; 2024-03 ; 2024-05
引用统计	被引频次：323[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1808
专题	第二临床医学院、附属第二医院、育英儿童医院附属第一医院
通讯作者	Lei, Bo; Mao, Cong
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 2, Key Lab Orthoped Zhejiang Prov, Dept Orthoped, Wenzhou 325027, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Peoples R China; 3.Xi An Jiao Tong Univ, Coll Stomatol, Key Lab Shaanxi Prov Craniofacial Precis Med Res, Xian 710000, Shaanxi, Peoples R China; 4.Wenzhou Med Univ, Affiliated Hosp 1, Ctr Diabet Foot, Wenzhou 325000, Peoples R China; 5.Xi An Jiao Tong Univ, Frontier Inst Sci & Technol, Xian 710054, Shaanxi, Peoples R China; 6.Xi An Jiao Tong Univ, Instrument Anal Ctr, Xian 710054, Shaanxi, Peoples R China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 骨科重点学科实验室
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 骨科重点学科实验室
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Wang, Chenggui,Wang, Min,Xu, Tianzhen,et al. Engineering Bioactive Self-Healing Antibacterial Exosomes Hydrogel for Promoting Chronic Diabetic Wound Healing and Complete Skin Regeneration[J]. THERANOSTICS,2019,9(1):65-76.
APA	Wang, Chenggui., Wang, Min., Xu, Tianzhen., Zhang, Xingxing., Lin, Cai., ... & Mao, Cong. (2019). Engineering Bioactive Self-Healing Antibacterial Exosomes Hydrogel for Promoting Chronic Diabetic Wound Healing and Complete Skin Regeneration. THERANOSTICS, 9(1), 65-76.
MLA	Wang, Chenggui,et al."Engineering Bioactive Self-Healing Antibacterial Exosomes Hydrogel for Promoting Chronic Diabetic Wound Healing and Complete Skin Regeneration".THERANOSTICS 9.1(2019):65-76.