Epigenome-wide DNA methylation analysis of myasthenia gravis

doi:10.1002/2211-5463.13656

科研成果详情

题名	Epigenome-wide DNA methylation analysis of myasthenia gravis
作者	Lin, Jingjing 1; Tao, Linshuang 2; Deng, Lu1; Zhou, Ruyi1; Lou, Shuyue1; Chen, Songfang 1; Chen, Xuanyu 1; Lu, Chunxing1; Li, Peijun1; Hu, Beilei1
发表日期	2023-07
发表期刊	FEBS open bio 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	DNA methylation epigenetics high throughput myasthenia gravis neuromuscular junction
其他关键词	PATHWAY ; CREB ; REGULATORS ; INHIBIT ; BINDING ; THYMUS ; CANCER
摘要	Myasthenia gravis (MG) is a common neuromuscular junction disorder and autoimmune disease mediated by several antibodies. Several studies have shown that genetic factors play an important role in MG pathogenesis. To gain insight into the epigenetic factors affecting MG, we report here genome-scale DNA methylation profiles of MG. DNA was extracted from eight MG patients and four healthy controls for genome-wide DNA methylation analysis using the Illumina HumanMethylation 850K BeadChip. Verification of pyrosequencing was conducted based on differential methylation positions. Subsequently, C2C12 and HT22 cell lines (derived from mouse) were treated with demethylation drugs. Transcribed mRNA of the screened differential genes was detected using quantitative real-time PCR. The control and MG group were compared, and two key probe positions were selected. The corresponding genes were CAMK1D and CREB5 (P < 0.05). Similarly, the myasthenic crisis (MC) and non-MC group were compared and four key probe positions were selected. The corresponding genes were SAV1, STK3, YAP1, and WWTR1 (P < 0.05). Subsequently, pyrosequencing was performed for verification, revealing that hypomethylation of CAMK1D was significantly different between the MG and control group (P < 0.001). Moreover, transcription of CREB5, PKD, YAP1, and STK3 genes in the C2C12 cells was downregulated (P < 0.05) after drug treatment, but only YAP1 mRNA was downregulated in HT22 cells (P < 0.05). This is the first study to investigate genome-scale DNA methylation profiles of MG using 850 K BeadChip. The identified molecular markers of methylation may aid in the prevention, diagnosis, treatment, and prognosis of MG.
资助项目	Yuying Children's Hospital of Wenzhou Medical University[SAHoWMU‐CR2017‐01‐21];Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University[19331204];
出版者	WILEY
ISSN	2211-5463
卷号	13 期号:7 页码:1375-1389
DOI	10.1002/2211-5463.13656
页数	15
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:001007175300001
收录类别	PUBMED ; SCIE ; SCOPUS
在线发表日期	2023-06
URL	查看原文
PubMed ID	37254650
SCOPUSEID	2-s2.0-85161671922
通讯作者地址	[Li, Peijun]Department of Neurology,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China ; [Hu, Beilei]Department of Neurology,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,China
Scopus学科分类	Biochemistry, Genetics and Molecular Biology (all)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/180611
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院其他_附属黄岩医院（台州市第一人民医院）
通讯作者	Li, Peijun; Hu, Beilei
作者单位	1.Department of Neurology,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,China; 2.Department of Nephrology,Taizhou First People's Hospital,Affiliated Huangyan Hospital of Wenzhou Medical University,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Lin, Jingjing,Tao, Linshuang,Deng, Lu,et al. Epigenome-wide DNA methylation analysis of myasthenia gravis[J]. FEBS open bio,2023,13(7):1375-1389.
APA	Lin, Jingjing., Tao, Linshuang., Deng, Lu., Zhou, Ruyi., Lou, Shuyue., ... & Hu, Beilei. (2023). Epigenome-wide DNA methylation analysis of myasthenia gravis. FEBS open bio, 13(7), 1375-1389.
MLA	Lin, Jingjing,et al."Epigenome-wide DNA methylation analysis of myasthenia gravis".FEBS open bio 13.7(2023):1375-1389.