Increased RNA editing sites revealed as potential novel biomarkers for diagnosis in primary Sjögren's syndrome

doi:10.1016/j.jaut.2023.103035

科研成果详情

题名	Increased RNA editing sites revealed as potential novel biomarkers for diagnosis in primary Sjögren's syndrome
作者	Wang, Xiaobing 1; Zhu, Lingxiao2; Ying, Senhong 3; Liao, Xin 4; Zheng, Junjie 2; Liu, Zhenwei5; Gao, Jianxia 5; Niu, Miaomiao 6; Xu, Xin 7; Zhou, Zihao 8; Xu, Huji 1,9,10; Wu, Jinyu 5
发表日期	2023-07
发表期刊	Journal of autoimmunity 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	ADAR1 Diagnosis Primary Sjögren's syndrome RNA editing RNA sequencing
其他关键词	CLASSIFICATION CRITERIA ; SUSCEPTIBILITY LOCI ; AMERICAN-COLLEGE ; DATA-DRIVEN ; INTERFERON ; EXPRESSION ; MUTATIONS ; AUTOPHAGY ; CONSENSUS ; PATIENT
摘要	Transcriptome-wide aberrant RNA editing has been shown to contribute to autoimmune diseases, but its extent and significance in primary Sjögren's syndrome (pSS) are currently poorly understood., We systematically characterized the global pattern and clinical relevance of RNA editing in pSS by performing large-scale RNA sequencing of minor salivary gland tissues obtained from 439 pSS patients and 130 non-pSS or healthy controls., Compared with controls, pSS patients displayed increased global RNA-editing levels, which were significantly correlated and clinically relevant to various immune features in pSS. The elevated editing levels were likely explained by significantly increased expression of adenosine deaminase acting on RNA 1 (ADAR1) p150 in pSS, which was associated with disease features. In addition, genome-wide differential RNA editing (DRE) analysis between pSS and non-pSS showed that most (249/284) DRE sites were hyper-edited in pSS, especially the top 10 DRE sites dominated by hyper-edited sites and assigned to nine unique genes involved in the inflammatory response or immune system. Interestingly, among all DRE sites, six RNA editing sites were only detected in pSS and resided in three unique genes (NLRC5, IKZF3 and JAK3). Furthermore, these six specific DRE sites with significant clinical relevance in pSS showed a strong capacity to distinguish between pSS and non-pSS, reflecting powerful diagnostic efficacy and accuracy., These findings reveal the potential role of RNA editing in contributing to the risk of pSS and further highlight the important prognostic value and diagnostic potential of RNA editing in pSS.
资助项目	Shanghai Municipal Key Clinical Specialty[shslczdzk02602];National Natural Science Foundation of China[32070594,82271816];Zhejiang Provincial Natural Science Foundation of China[LY23C060002];National Key Research and Development Project[2018AAA0100302];
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
ISSN	0896-8411
EISSN	1095-9157
卷号	138
DOI	10.1016/j.jaut.2023.103035
页数	11
WOS类目	Immunology
WOS研究方向	Immunology
WOS记录号	WOS:001009727700001
收录类别	PUBMED ; SCIE ; SCOPUS
在线发表日期	2023-05
URL	查看原文
PubMed ID	37216868
SCOPUSEID	2-s2.0-85159584216
通讯作者地址	[Wu, Jinyu]Institute of Genomic Medicine,Wenzhou Medical University,Wenzhou,325000,China ; [Xu, Huji]Department of Rheumatology and Immunology,Shanghai Changzheng Hospital,Naval Medical University,Shanghai,200003,China
Scopus学科分类	Immunology and Allergy;Immunology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/180548
专题	附属第一医院基因组医学研究院
通讯作者	Xu, Huji; Wu, Jinyu
作者单位	1.Department of Rheumatology and Immunology,Shanghai Changzheng Hospital,Naval Medical University,Shanghai,China; 2.Department of Rheumatology and Immunology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 3.Precision Medicine Center,The Second Affiliated Hospital of Chongqing Medical University,Chongqing,China; 4.The Center for Clinical Molecular Medical Detection,The First Affiliated Hospital of Chongqing Medical University,Chongqing,China; 5.Institute of Genomic Medicine,Wenzhou Medical University,Wenzhou,China; 6.Ningbo Health Gene Technologies Co,Ningbo,China; 7.Shandong Cancer Hospital and Institute,Jinan,China; 8.Department of Clinical Laboratory,The Third People's Hospital of Shenzhen,Southern University of Science and Technology,National Clinical Research Center for Infectious Diseases,Shenzhen,China; 9.Peking-Tsinghua Center for Life Sciences,Tsinghua University,Beijing,China; 10.School of Clinical Medicine,Tsinghua University,Beijing,China
通讯作者单位	基因组医学研究院
推荐引用方式 GB/T 7714	Wang, Xiaobing,Zhu, Lingxiao,Ying, Senhong,et al. Increased RNA editing sites revealed as potential novel biomarkers for diagnosis in primary Sjögren's syndrome[J]. Journal of autoimmunity,2023,138.
APA	Wang, Xiaobing., Zhu, Lingxiao., Ying, Senhong., Liao, Xin., Zheng, Junjie., ... & Wu, Jinyu. (2023). Increased RNA editing sites revealed as potential novel biomarkers for diagnosis in primary Sjögren's syndrome. Journal of autoimmunity, 138.
MLA	Wang, Xiaobing,et al."Increased RNA editing sites revealed as potential novel biomarkers for diagnosis in primary Sjögren's syndrome".Journal of autoimmunity 138(2023).