Structural Analysis and Effects of Exopolysaccharide (LHEPS-3) from Lactobacillus helveticus MB2-1 on Human Hepatoma HepG-2 Cells: Inhibition of Proliferation, Migration and Angiogenesis

doi:10.1134/S1068162023030251

科研成果详情

题名	Structural Analysis and Effects of Exopolysaccharide (LHEPS-3) from Lactobacillus helveticus MB2-1 on Human Hepatoma HepG-2 Cells: Inhibition of Proliferation, Migration and Angiogenesis
作者	Xiaomeng Wang 1; Ji, Juan 1; Xu, Mengjia 1,2; Rui, Xin 1; Zhang, Qiuqin 1; Dong, Mingsheng 1; Li, Wei 1
发表日期	2023-06
发表期刊	RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	Lactobacillus helveticus MB2-1 exopolysaccharide (EPS) structural analysis Inhibition of proliferation
其他关键词	ANTITUMOR-ACTIVITY ; ANTIOXIDANT ACTIVITIES ; IN-VITRO ; CANCER ; POLYSACCHARIDE ; APOPTOSIS ; INVASION
摘要	Recently, we published the extraction, purification, and characterization of exopolysaccharide (EPS) from Lactobacillus helveticus MB2-1. In this study, the selective cytotoxic effects of LHEPS-3 fraction isolated from EPS of L. helveticus MB2-1 against human HepG-2 liver cancer cells and human HT29 colon cancer cells were evaluated. Results indicated that LHEPS-3 could significantly inhibit the proliferation of HepG-2 and HT29 cells in a dose- and time-dependent manner, and the inhibitory effects on HepG-2 cells were better than HT29 cells. In addition, with the increase of LHEPS-3 concentration (0-600 mu g/mL), the incubation time (0-48 h), and the migration rate of HepG-2 cells gradually decreased. LHEPS-3 could also inhibit the neovascularization of chicken embryo chorioallantoic membrane (CAM) in a dose-dependent manner. Furthermore, the structure of LHEPS-3 was clarified by methylated analysis, one- and two-dimensional nuclear magnetic resonance (1D and 2D NMR), thereby the repeating units of LHEPS-3 were predicted.
资助项目	National Natural Science Foundation of China[31871771,32101921,U1903108];Natural Science Foundation of Jiangsu Province[BK20201320];
出版者	MAIK NAUKA/INTERPERIODICA/SPRINGER
ISSN	1068-1620
EISSN	1608-330X
卷号	49 期号:3 页码:616-628
DOI	10.1134/S1068162023030251
页数	13
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Organic
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000986155200012
收录类别	SCIE ; SCOPUS
在线发表日期	2023-05
URL	查看原文
SCOPUSEID	2-s2.0-85159105358
通讯作者地址	[Li, Wei]College of Food Science and Technology,Nanjing Agricultural University,Jiangsu,Nanjing,210095,China
Scopus学科分类	Biochemistry;Organic Chemistry
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/180129
专题	其他_附属慈溪医院（慈溪市人民医院）
通讯作者	Li, Wei
作者单位	1.College of Food Science and Technology,Nanjing Agricultural University,Jiangsu,Nanjing,210095,China; 2.Affiliated Cixi Hospital,Wenzhou Medical University,Zhejiang,Cixi,315300,China
推荐引用方式 GB/T 7714	Xiaomeng Wang,Ji, Juan,Xu, Mengjia,et al. Structural Analysis and Effects of Exopolysaccharide (LHEPS-3) from Lactobacillus helveticus MB2-1 on Human Hepatoma HepG-2 Cells: Inhibition of Proliferation, Migration and Angiogenesis[J]. RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY,2023,49(3):616-628.
APA	Xiaomeng Wang., Ji, Juan., Xu, Mengjia., Rui, Xin., Zhang, Qiuqin., ... & Li, Wei. (2023). Structural Analysis and Effects of Exopolysaccharide (LHEPS-3) from Lactobacillus helveticus MB2-1 on Human Hepatoma HepG-2 Cells: Inhibition of Proliferation, Migration and Angiogenesis. RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, 49(3), 616-628.
MLA	Xiaomeng Wang,et al."Structural Analysis and Effects of Exopolysaccharide (LHEPS-3) from Lactobacillus helveticus MB2-1 on Human Hepatoma HepG-2 Cells: Inhibition of Proliferation, Migration and Angiogenesis".RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY 49.3(2023):616-628.