Identification and expression profiling of Spodoptera litura OR18, a gene with a potential role in olfactory recognition

doi:10.1111/eea.12151

科研成果详情

题名	Identification and expression profiling of Spodoptera litura OR18, a gene with a potential role in olfactory recognition
作者	Zheng, Kaidi1,2; Lin, Xinda 3; Du, Yongjun1
发表日期	2014-02
发表期刊	ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA 影响因子和分区
语种	英语
原始文献类型	Article
关键词	olfactory receptor SlituOR18 evolution geographic variation diurnal rhythm Noctuidae Lepidoptera
其他关键词	DROSOPHILA ODORANT RECEPTORS ; TOBACCO CUTWORM MOTH ; PHEROMONE RECEPTION ; MEMBRANE TOPOLOGY ; SEX-PHEROMONES ; RESPONSES ; PROTEINS ; SYSTEM ; MODEL
摘要	The full-length cDNA of the gene SlituOR18, encoding a candidate olfactory receptor in the antennae of the tobacco cutworm, Spodoptera litura (Fabricius) (Lepidoptera Noctuidae), was identified through homology cloning strategies using RACE (rapid amplification of cDNA ends) and PCR (polymerase chain reaction). The protein of SlituOR18 shared >80% sequence identity and the same seven transmembrane domains with other olfactory receptor 18 (OR18) proteins sequenced from noctuid moths. SlituOR18 also had a similar structure to SlituORco, encoding the olfactory co-receptor in S.litura. The sequence between transmembrane segments IV and V was longer than other sequences between transmembrane segments, and the N-terminus was intracytoplasmic. Analysis by qRT-PCR showed SlituOR18 was predominantly expressed in adult moths and there was higher expression in female antennae than in male antennae. Weak expression of SlituOR18 was observed in eggs and no expression was observed in the antennae of fourth instars and 5-day-old pupae. In situ hybridization experiments revealed that expression of these receptor types was clearly restricted to the bases of trichodea-type olfactory sensilla (sensillum trichodeum), that are known to contain neurons sensitive to food odor or pheromones. Our data demonstrated that both SlituORco and SlituOR18 showed diurnal changes in their relative expression level. Expression of SlituOR18 varied among geographic populations of S.litura that had been trapped in the field using synthetic pheromone lures. The mRNA expression level of SlituOR18 was similar among S.litura populations from Sichuan, Guangxi, and Hunan, and higher than in populations from Shanghai and Ningbo. We suggest that OR18 could play a critical role in olfaction in noctuid moths and is a potential target for novel pest management strategies in the future.
资助项目	Special Fund for Agro-scientific Research in the Public Interest in China [201203036]; Natural Science Foundation of ZhejiangNatural Science Foundation of Zhejiang Province [D3080388]
出版者	WILEY
出版地	HOBOKEN
ISSN	0013-8703
EISSN	1570-7458
卷号	150 期号:2 页码:123-135
DOI	10.1111/eea.12151
页数	13
WOS类目	Entomology
WOS研究方向	Entomology
WOS记录号	WOS:000329778100003
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-84892561087
通讯作者地址	[Du, Yongjun]Institute of Health and Environmental Ecology,Wenzhou Medical University,China
Scopus学科分类	Ecology, Evolution, Behavior and Systematics;Insect Science
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17484
专题	公共卫生学院_健康与环境生态研究所检验医学院（生命科学学院、生物学实验教学中心）_生物化学与分子生物学系基础医学院（机能实验教学中心）_生物科学系_生物化学与分子生物学
通讯作者	Du, Yongjun
作者单位	1.Institute of Health and Environmental Ecology,Wenzhou Medical University,University Town,China; 2.Department of Biochemistry,Wenzhou Medical University,China; 3.College of Life Sciences,China Jiliang University,China
第一作者单位	健康与环境生态研究所; 生物化学与分子生物学
通讯作者单位	健康与环境生态研究所
第一作者的第一单位	健康与环境生态研究所
推荐引用方式 GB/T 7714	Zheng, Kaidi,Lin, Xinda,Du, Yongjun. Identification and expression profiling of Spodoptera litura OR18, a gene with a potential role in olfactory recognition[J]. ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA,2014,150(2):123-135.
APA	Zheng, Kaidi, Lin, Xinda, & Du, Yongjun. (2014). Identification and expression profiling of Spodoptera litura OR18, a gene with a potential role in olfactory recognition. ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA, 150(2), 123-135.
MLA	Zheng, Kaidi,et al."Identification and expression profiling of Spodoptera litura OR18, a gene with a potential role in olfactory recognition".ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA 150.2(2014):123-135.