Expression patterns of three regulation enzymes in glycolysis in esophageal squamous cell carcinoma: association with survival

doi:10.1007/s12032-014-0118-1

科研成果详情

题名	Expression patterns of three regulation enzymes in glycolysis in esophageal squamous cell carcinoma: association with survival
作者	Li, Wenfeng1; Xu, Zhengyang 2; Hong, Junfeng 3; Xu, Yunsheng4
发表日期	2014-09
发表期刊	MEDICAL ONCOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Esophageal squamous cell carcinoma PKM2 HK1 PFKB
其他关键词	COLON-CANCER CELLS ; PKM2 EXPRESSION ; 5-FLUOROURACIL ; METABOLISM ; HK1
摘要	Enhanced glycolysis is a common trait of many types of human cancers. This study was to detect the expression pattern of three regulatory enzymes during glycolysis in esophageal squamous cell carcinoma (ESCC) and to investigate their correlation with patients' outcome based on banked pathology material. A total of 141 surgically resected specimens of primary ESCC patients without prior treatments were retrospectively recruited from the First Affiliated Hospital of Wenzhou Medical College Hospital from 2007 to 2009. Expression of HK1, PFKB, and PKM2 in ESCC specimens was analyzed by immunohistochemical staining and Western blotting analysis. HK1-shRNA was used to knock down HK1 expression in ESCC cells, and the functional significance was assessed by CCK8 assay. It was found that the expression of two glycolytic enzymes, HK1 and PKM2, was associated with disease progression, invasion, and poor survival of patients with ESCC. Silence of HK1-inhibited cell proliferation in vitro and suppressed phospho-S6 kinase expression. Our findings suggest that activation of key enzymes in glycolysis might serve as potential therapeutic targets and/or prognostic factors for patients with ESCC.
出版者	HUMANA PRESS INC
出版地	TOTOWA
ISSN	1357-0560
EISSN	1559-131X
卷号	31 期号:9 页码:118
DOI	10.1007/s12032-014-0118-1
页数	8
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000341835700007
收录类别	SCIE ; PUBMED
URL	查看原文
PubMed ID	25064730
通讯作者地址	[Xu, Yunsheng]Wenzhou Med Coll, Affiliated Hosp 1, Dept Dermatovenereol, Wenzhou 325000, Zhejiang, Peoples R China.
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17482
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_皮肤病与性病学_皮肤性病科
通讯作者	Xu, Yunsheng
作者单位	1.Wenzhou Med Coll, Affiliated Hosp 1, Dept Radiat Oncol, Wenzhou 325000, Zhejiang, Peoples R China; 2.Peoples Hosp Yinzhou Dist, Dept Chemoradiotherapy, Ningbo 315000, Zhejiang, Peoples R China; 3.PLA, Fuzhou Gen Hosp, Dept Ultrosound, Fuzhou 350025, Fujian, Peoples R China; 4.Wenzhou Med Coll, Affiliated Hosp 1, Dept Dermatovenereol, Wenzhou 325000, Zhejiang, Peoples R China
第一作者单位	附属第一医院
通讯作者单位	第一临床医学院（信息与工程学院）、附属第一医院_皮肤病与性病学_皮肤性病科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Li, Wenfeng,Xu, Zhengyang,Hong, Junfeng,et al. Expression patterns of three regulation enzymes in glycolysis in esophageal squamous cell carcinoma: association with survival[J]. MEDICAL ONCOLOGY,2014,31(9):118.
APA	Li, Wenfeng, Xu, Zhengyang, Hong, Junfeng, & Xu, Yunsheng. (2014). Expression patterns of three regulation enzymes in glycolysis in esophageal squamous cell carcinoma: association with survival. MEDICAL ONCOLOGY, 31(9), 118.
MLA	Li, Wenfeng,et al."Expression patterns of three regulation enzymes in glycolysis in esophageal squamous cell carcinoma: association with survival".MEDICAL ONCOLOGY 31.9(2014):118.