Zinc Deficiency Exacerbates Behavioral Impediments and Dopaminergic Neuron Degeneration in a Mouse Model of Parkinson Disease

doi:10.1016/j.tjnut.2022.11.006

科研成果详情

题名	Zinc Deficiency Exacerbates Behavioral Impediments and Dopaminergic Neuron Degeneration in a Mouse Model of Parkinson Disease
作者	Zhang, Xiong1,2; Wang, Ke1,3; Ren, Xiao-Li4; Zhang, Meng-Di2; Wu, Kai-Nian2; Wu, Han2; Chu, Zhong-Wei 2; Liu, Shu-Shu 2; Jiang, Xiao-Xia 2; Zhu, Jian-Hong1,2; Wu, Hong-Mei2
发表日期	2023-01
发表期刊	JOURNAL OF NUTRITION 影响因子和分区
语种	英语
原始文献类型	Article
关键词	behavior dopaminergic neuron Parkinson disease transcriptomics zinc
其他关键词	PROTEIN ; RISK ; SYNPHILIN-1 ; DEATH ; THAP1 ; MICE ; IRON
摘要	Background: Circulating zinc (Zn) concentrations are lower than normal in patients with Parkinson disease (PD). It is unknown whether Zn deficiency increases the susceptibility to PD. Objectives: The study aimed to investigate the effect of dietary Zn deficiency on behaviors and dopaminergic neurons in a mouse model of PD and to explore potential mechanisms. Methods: Male C57BL/6J mice aged 8-10 wk were fed Zn adequate (ZnA; 30 mu g/g) or Zn deficient (ZnD; <5 mu g/g) diet throughout the experiments. Six weeks later 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was injected to generate the PD model. Controls were injected with saline. Thus, 4 groups (Saline-ZnA, Saline-ZnD, MPTP-ZnA, and MPTP-ZnD) were formed. The experiment lasted 13 wk. Open field test, rotarod test, immunohistochemistry, and RNA sequencing were performed. Data were analyzed with t-test, 2-factor ANOVA, or Kruskal-Wallis test. Results: Both MPTP and ZnD diet treatments led to a significant reduction in blood Zn concentrations (PMPTP = 0.012, PZn = 0.014), reduced total distance traveled (PMPTP < 0.001, PZn = 0.031), and affected the degeneration of dopaminergic neurons in the substantia nigra (PMPTP < 0.001, PZn = 0.020). In the MPTP-treated mice, the ZnD diet significantly reduced total distance traveled by 22.4% (P = 0.026), decreased latency to fall by 49.9% (P = 0.026), and reduced dopaminergic neurons by 59.3% (P = 0.002) compared with the ZnA diet. RNA sequencing analysis revealed a total of 301 differentially expressed genes (156 upregulated; 145 downregulated) in the substantia nigra of ZnD mice compared with ZnA mice. The genes were involved in a number of processes, including protein degradation, mitochondria integrity, and alpha-synuclein aggregation. Conclusions: Zn deficiency aggravates movement disorders in PD mice. Our results support previous clinical observations and suggest that appropriate Zn supplementation may be beneficial for PD.
资助项目	Zhejiang Provincial Natural Science Foundation [LGF21H260011, LD19H090001, LZ19H090002]; Wenzhou Municipal Science and Technology Bureau [Y20190189]; Wenzhou Medical University Scien- ti fi c Research Foundation [XZ2022001]
出版者	ELSEVIER SCIENCE INC
ISSN	0022-3166
EISSN	1541-6100
卷号	153 期号:1 页码:167-175
DOI	10.1016/j.tjnut.2022.11.006
页数	9
WOS类目	Nutrition & Dietetics
WOS研究方向	Nutrition & Dietetics
WOS记录号	WOS:000948081200001
收录类别	SCIE ; SCOPUS ; PUBMED
在线发表日期	2023-02
URL	查看原文
PubMed ID	3691345
SCOPUSEID	2-s2.0-85150123843
通讯作者地址	[Wu, Hong-Mei]Institute of Nutrition and Diseases,Department of Preventive Medicine,Wenzhou Medical University,Zhejiang,Wenzhou,China ; [Zhu, Jian-Hong]Institute of Geriatric Neurology,Department of Neurology,The Second Affiliated Hospital and Yuying Children's Hospital,Wenzhou Medical University,Zhejiang,Wenzhou,China
Scopus学科分类	Medicine (miscellaneous);Nutrition and Dietetics
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/174611
专题	附属第二医院公共卫生学院_预防医学系实验动物中心
通讯作者	Zhu, Jian-Hong; Wu, Hong-Mei
作者单位	1.Institute of Geriatric Neurology,Department of Neurology,The Second Affiliated Hospital and Yuying Children's Hospital,Wenzhou Medical University,Zhejiang,Wenzhou,China; 2.Institute of Nutrition and Diseases,Department of Preventive Medicine,Wenzhou Medical University,Zhejiang,Wenzhou,China; 3.Department of Neurology,Lishui Hospital of Zhejiang University (the Central Hospital of Lishui),Zhejiang,Lishui,China; 4.Laboratory Animal Center,Wenzhou Medical University,Zhejiang,Wenzhou,China
第一作者单位	附属第二医院; 公共卫生学院_预防医学系
通讯作者单位	公共卫生学院_预防医学系; 附属第二医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Zhang, Xiong,Wang, Ke,Ren, Xiao-Li,et al. Zinc Deficiency Exacerbates Behavioral Impediments and Dopaminergic Neuron Degeneration in a Mouse Model of Parkinson Disease[J]. JOURNAL OF NUTRITION,2023,153(1):167-175.
APA	Zhang, Xiong., Wang, Ke., Ren, Xiao-Li., Zhang, Meng-Di., Wu, Kai-Nian., ... & Wu, Hong-Mei. (2023). Zinc Deficiency Exacerbates Behavioral Impediments and Dopaminergic Neuron Degeneration in a Mouse Model of Parkinson Disease. JOURNAL OF NUTRITION, 153(1), 167-175.
MLA	Zhang, Xiong,et al."Zinc Deficiency Exacerbates Behavioral Impediments and Dopaminergic Neuron Degeneration in a Mouse Model of Parkinson Disease".JOURNAL OF NUTRITION 153.1(2023):167-175.